SNP Identifizierung für eine gezielte Genotypisierung von Ja

The ability of species to adapt to different environments resides in their genetic diversity. This diversity, most commonly manifested as Single Nucleotide Polymorphisms (SNPs), can provide clues to the adaptive processes and population histories that have played roles in species’ evolution. A number of different techniques for identifying SNPs have been developed but all have their limitations. TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic technology that can identify polymorphisms in a target gene by heteroduplex analysis. A variation of this technique (EcoTILLING) represents a means to determine the extent of natural variation in selected genes. EcoTILLING and TILLING may be a cost effective approach for haplotyping and SNPs discovery. The objectives are: i) to assess genetic variation in J. curcas accessions and in closely related species. ii) to assess EcoTILLING and TILLING as a reliable cost effective method to detect SNPs in a large number of J. curcas accessions, related species and induced mutants. iii) to use EcoTILLING and TILLING as a reverse genetic tool for functional analysis of J. curcas genes. The foreseen activities will be as follows: • Selection of candidate genes (genes involved in oil production, toxicity and adaptation to stress defence response) of J. curcas. • Assessment of haplotype diversity detected through EcoTILLING and TILLING in a wide range of germplasm of Jatropha and in EMS induced mutants. • Search for allelic diversity and assessment of EcoTILING and TILLING as methods to discover novel alleles.