Vera Pfanzagl
Dipl.-Ing. Vera Pfanzagl Ph.D.
Institute of Biochemistry
Standort Muthgasse 18, 1190 Wien
Email vera.pfanzagl@boku.ac.at
Tel: +43 1 47654-77279
ORCID: 0000-0002-9361-8365
AuthorId : 57200222267
ResearcherId : AAE-9025-2019
Forschungsschwerpunkt
We study the structure-function relationship of human heme peroxidases using a combination of biochemical analysis, X-ray crystallography and molecular biology. Currently we focus on three proteins, Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) from the innate immune response and Thyroid peroxidas (TPO), the main enzyme catalyzing thyroid hormone production. We want to unravel the fine differences between MPO and EPO to understand their divergent function and to find exploitable structural traits for design of new selective inhibitors. The membrane bound TPO on the other hand, is not only essential for hormone biosynthesis but also a main auto-antigen in Hashimotos disease, where constant attack by auto-antibodies leads to a degradation of the thyroid gland. Here we focus on solving the crystal structure and understanding the properties of TPO in detail to understand the high prevalence of TPO associated autoimmune diseases.
Laufbahn
- 2024 Elise-Richter Fellow (Department of Chemistry, BOKU)
- 2019 - 2024 Post-Doc (Department of Chemistry, BOKU)
- 2015 - 2019 Dissertation withing the international PhD programme BioToP at the Department of Chemistry, BOKU
- 2015 Technician at the Department for Applied Genetics and Cell Biology, BOKU
- 2014 FEMtech internship at the Department for Applied Genetics and Cell Biology, BOKU
- 2011 master thesis at the NIBR Novartis, Switzerland
- 2011 - 2014 Master Biotechnology (BOKU)
- 2007 - 2011 Bachelor Food- and Biotechnology (BOKU)
Projekte
Es wurden 0 Projekte gefunden.
Publikationen
Wissentransfer in die Gesellschaft
Medienbeiträge
Vorträge
Es wurden 29 gefunden.
: 2025 - Mechanistic insights into heme iron photoreduction: influence of temperature on redox and structural states
Art: Lecture - Ausgewählte Präsentation
Autoren: Pfanzagl, V.
: 35th European Crystallographic Meeting 2025
: 25.08.2025 - 29.08.2025 | : Posen, Posen, Polen | : European Crystallographic Association
: 2024 - Myeloperoxidase dimerization in solution is mediated by interface glycans
Art: Poster - Ausgewählte Präsentation
Autoren: Leitgeb, U; Furtmüller, PG; Obinger, C; Pfanzagl, V
: 34th European Crystallographic Meeting 2024
: 26.08.2024 - 30.08.2024 | : Padua, Italien | : European Crystallographic Association
: 2023 - Reactivity and protein-protein interaction of human peroxidasin 1
Art: Vortrag - Ausgewählte Präsentation
Autoren: Furtmüller, PG, Paumann-Page, M; Pfanzagl, V; Hofbauer, S; Obinger, C
: 12th International Human Peroxidase Meeting 2023
: 30.08.2023 - 02.09.2023 | : Budapest, Hungary | : Budapest, Ungarn | : Semmelweis Egyetem
: 2023 - Reconstitution and linkage of heme in thyroid peroxidase post-purification
Art: Poster - Ausgewählte Präsentation
Autoren: Parzer, B; Furtmüller, PG; Obinger, C; Pfanzagl, V
: 12th International Human Peroxidase Meeting 2023
: 30.08.2023 - 02.09.2023 | : Budapest, Hungary | : Budapest, Ungarn | : Semmelweis Egyetem
: 2023 - First crystal structure of eosinophil peroxidase reveals suptile distinctions in the active site architecture and processing
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Leitgeb, U; Grünwald-Gruber, C; Furtmüller, PG; Obinger, C
: 12th International Human Peroxidase Meeting 2023
: 30.08.2023 - 02.09.2023 | : Budapest, Hungary | : Budapest, Ungarn | : Semmelweis Egyetem
: 2023 - The inhibitory protein SPIN reduces the halide oxidation rate of myeloperoxidase but leaves the substrate binding site unaffected
Art: Vortrag - Ausgewählte Präsentation
Autoren: Leitgeb, U; Brito, JA; Furtmüller, PG; Obinger, C; Pfanzagl, V
: 12th International Human Peroxidase Meeting 2023
: 30.08.2023 - 02.09.2023 | : Budapest, Hungary | : Budapest, Ungarn | : Semmelweis Egyetem
: 2023 - Testing the incorporation efficiency of phenylalanine derivatives with dual fluorescent reporter proteins
Art: Poster - Ausgewählte Präsentation
Autoren: Terzer, F; Leitgeb, U; Marin, Z; Kani, A.A; Wiltschi, B; Pfanzagl, V; Obinger, C
: 12th International Human Peroxidase Meeting 2023
: 30.08.2023 - 02.09.2023 | : Budapest, Hungary | : Budapest, Ungarn | : Semmelweis Egyetem
: 2022 - Biochemistry of Enzymes Involved in the Coproporphyrin-Dependent Heme Biosynthesis Pathway of Gram-Positive Bacteria
Art: Vortrag - Keynote Speaker (eingeladener Vortrag)
Autoren: Hofbauer, S; Gabler, T; Patil, G; Michlits, H; Pfanzagl, V; Obinger, C; Furtmüller, PG
: Gordon Research Conference - Chemistry and Biology of Tetrapyrroles
: 17.07.2022 - 22.07.2022 | : Newport, Rhode Island, USA
: 2022 - Structural and mechanistic insights in dimeric chlorite dismutase - Impact of pH and the dynamics of the catalytic arginine
Art: Vortrag - Ausgewählte Präsentation
Autoren: Schmidt, D; Serra, I; Hofbauer, S; Pfanzagl, V; Van Doorslaer, S; Obinger, C; Furtmüller, PG
: 16th European Biological Inorganic Chemistry 2022
: 17.07.2022 - 21.07.2022 | : Grenoble, France
: 2022 - New insights into B-class dye-decolorizing peroxidases: tracing radical sites with electron paramagnetic resonance
Art: Vortrag - Keynote Speaker (eingeladener Vortrag)
Autoren: Van Doorslaer, S; Nys, K; Furtmüller, PG; Obinger, C; Pfanzagl, V;
: 12th International Conference on Porphyrins and Phtalocyanines ICPP-12 2022
: 10.07.2022 - 15.07.2022 | : Madrid, Spain
: 2022 - Reactivity of peroxidases and chlorite dismutases with chlorite and hypochlorite: a spectroscopic investigation of short-lived intermediates trapped by fast freeze-quenching
Art: Vortrag - Ausgewählte Präsentation
Autoren: Serra, I; Schmidt, D; Hofbauer, S; Pfanzagl, V; García-Rubio, I; Furtmüller, PG; Obinger C; and Van Doorslaer, V;
: The 55th Annual International Meeting of the ESR Spectroscopy Group of the Royal Society of Chemistry 2022
: 06.06.2022 - 10.06.2022 | : St. Andrews, Scotland, UK
: 2022 - X-ray induced reduction of heme metal centers is protein-independent
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Beale, JH; Michlits, H; Schmidt, D; Gabler, T; Obinger, C; Djinovic-Carugo, K; Hofbauer, S;
: 33rd European Crystallographic Meeting
: 23.08.2022 - 27.08.2022 | : Versailles, France
: 2021 - Coproporphyrin ferrochelatase – study of relevant residues for substrate and product binding
Art: Vortrag - Ausgewählte Präsentation
Autoren: Gabler, T; Pfanzagl, V; Obinger, C; Furtmüller PG; Hofbauer, S
: International Conference on Porphyrins and Phthalocyanines - ICPP11
: 28.06.2021 - 03.07.2021 | : Online
: 2019 - X-ray induced redcution kinetics of heme metal centers is protein-independent
Art: Poster - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Beale, J; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, K; Obinger, C; Hofbauer, S
: 11th Human Peroxidase Meeting
: 04.09.2019 - 07.09.2019 | : Brno, Czech Republic
: 2019 - Posttranslational Modification of Heme in Peroxidases - Impact on Structure and Function
Art: Vortrag - Ausgewählte Präsentation
Autoren: Furtmüller, PG; Nicolussi, A; Sevcnikar, B; Paumann-Page, M; Auer, M; Zamocky, M; Pfanzagl, V; Hofbauer, S; Obinger, C
: 19th International Conference on Biological Inorganic Chemistry 2019
: 11.08.2019 - 16.08.2019 | : Interlaken, Switzerland
: 2019 - An actinobacterial representative sheds a new light on the reaction mechanism of coproheme decarboxylases
Art: Poster - Ausgewählte Präsentation
Autoren: Michlits, H; Pfanzagl, V; Furtmüller, PG; Djinovic-Carugo, K; Obinger, C; Hofbauer, S
: 19th International Conference on Biological Inorganic Chemistry 2019
: 11.08.2019 - 16.08.2019 | : Interlaken, Switzerland
: 2019 - YfeX - A new player involved in the redox gated potassium efflux pump KefC
Art: Poster - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Schwaiger, L; Michlits, H; Hofbauer, S; Obinger, C
: Gordon Research Conference: Mechanisms of Membrane Transport
: 23.06.2019 - 28.06.2019 | : New London, UK
: 2019 - X-ray induced redcution kinetics of heme metal centers is protein-independent - implications for structural studies of redox sensitive proteins
Art: Poster - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Beale, J; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, K; Obinger, C; Hofbauer, S
: 19th International Conference on Biological Inorganic Chemistry 2019
: 11.08.2019 - 16.08.2019 | : Interlaken, Switzerland
: 2019 - X-ray induced reduction of heme metal centers is protein-independent – implications for structural studies of redox sensitive proteins
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Beale, JH; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, C; Obinger, C; Hofbauer, S
: Hot Topics in Crystallography 4
: 01.10.2019 - 06.10.2019 | : Dubrovnik, Croatia
: 2019 - Molecular mechanism of coproheme to heme b conversion
Art: Vortrag - Ausgewählte Präsentation
Autoren: Hofbauer, S; Milazzo, L; Gabler, T; Pühringer, D; Pfanzagl, V; Djinovic-Carugo, K; Furtmüller, PG; Obinger, C; Smulevich, G
: 19th International Conference on Biological Inorganic Chemistry 2019
: 11.08.2019 - 16.08.2019 | : Interlaken, Switzerland
: 2018 - Kinetic and spectroscopic characterization of EfeB
Art: Poster - Ausgewählte Präsentation
Autoren: Michlits, H; Pfanzagl, V; Schwaiger, L; Furtmüller, PG; Hofbauer, S; Obinger, C
: OxiZymes 2018
: 08.07.2018 - 10.07.2018 | : Belfast, Northern Ireland, UK | : QUB
: 2018 - Mechanistic Studies on Coproheme Decarboxylase
Art: Poster - Ausgewählte Präsentation
Autoren: Hofbauer, S; Milazzo, L; Mlynek, G; Gabler, T; Howes, BD; Pfanzagl, V; Djinović-Carugo, K; Furtmüller, PG; Smulevich, G; Obinger, C
: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
: 01.07.2018 - 06.07.2018 | : Munich, Germany
: 2018 - Crystal and Electronic Structure of Compound I in Dye-decolorizing Peroxidases
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Hofbauer, S; Beale, J; Mlynek, G; Michlits, H; Nys, K; Van Doorslaer, S; Djinovic-Carugo, K; Furtmüller, PG; Obinger, C
: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
: 01.07.2018 - 06.07.2018 | : Munich, Germany
: 2018 - On Compound I formation in B-type dye decolorizing peroxidases - lessons from structural and kinetic studies.
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Nys, K; Mlynek, G; Djinović-Carugo, K; Van Doorslaer, S; Furtmüller, PG; Hofbauer, S; Obinger, C
: OxiZymes 2018
: 08.07.2018 - 10.07.2018 | : Belfast, Northern Ireland, UK | : QUB
: 2018 - Coproheme decarboxylases – phylogenetic prediction versus biochemical analysis.
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Holcik, L; Maresch, D; Michlits, H; Furtmüller, PG; Obinger, C; Hofbauer, S
: OxiZymes 2018
: 08.07.2018 - 10.07.2018 | : Belfast, Northern Ireland, UK | : QUB
: 2017 - Roles of the distal Asp-Arg pair in Compound I formation of K. pneumoniae dye-decolorizing peroxidase
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Hofbauer, S; Nys, K; Djinovic-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmuller, PG; Obinger, C
: International Conference on Bioinorganic Chemistry ICBIC-18 2017
: 31.07.2017 - 04.08.2017 | : Florianopolis, Brazil
: 2017 - Roles of the distal Asp-Arg pair in compound I formation of K. pneumoniae dye-decolorizing peroxidase
Art: Poster - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Hofbauer, S; Nys, K; Djinović-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmüller, P.G; and Obinger, C
: International Conference on Bioinorganic Chemistry ICBIC-18 2017
: 31.07.2017 - 04.08.2017 | : Florianopolis, Brazil
: 2016 - Dye-decolorizing peroxidases – a new heme-peroxidase family with ancient roots
Art: Poster - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Furtmüller, PG; Obinger, C
: 13th European Biological Inorganic Chemistry Conference EuroBIC 2016
: 28.08.2016 - 01.09.2016 | : Budapest, Hungary
: 2016 - Dye decolorizing peroxidases – a new heme-peroxidase family with ancient roots
Art: Vortrag - Ausgewählte Präsentation
Autoren: Pfanzagl, V; Schaffner, I; Hofbauer, S; Furtmüller, PG; Obinger, C
: 17th European Congress on Biotechnology ECB17 2016
: 03.07.2016 - 06.07.2016 | : Krakow, Poland | : European Federation of Biotechnology