5D-Click-Druck zur Herstellung von Strukturen mit Mechanischen und Funktionellen Gradient
Abstract
Mechanical and functional gradients are reasons for the abundance of functionalities and extraordinary mechanical properties in nature. Mechanical gradients are spatial smooth transitions from mechanically weak to strong structures resulting in materials with remarkable mechanical performance. In case of the in vivo cell environment, the extra-cellular matrix, there are not only mechanical gradients present but also functional gradients, such as an increasing concentration of a bio-active molecule in one dimension. These gradients play an important role in the organization of cells into functional tissues and organs. The imitation of these multidimensional structures by biocompatible and shapeable materials in a straightforward way is a critical challenge that will be addressed in this proposal. The research hypothesis is the development of a novel gradient printing approach, named 5D Click Printing, combining cutting-edge bioprinting technology with state-of-the-art materials and crosslinking chemistry. This will be realized by using functional nanocellulose and polyoxazoline as ink formulations to produce 3D objects with mechanical (+1D) and functional gradients (+1D). The proposed ink formulations are based on functional cellulose nanofibrils and polyoxazolines. These materials were chosen because of their established biocompatibilities, printabilities and the resemblance to the two main components of the extra-cellular matrices, fiber-forming proteins and non-fibrous glycoproteins. The functional groups on the polymers were carefully selected to allow gelation by spontaneous click chemistry, which can be conducted in the presence of living cells. The 5D Click Printing technology will be further developed to fabricate multidimensional hydrogels with various functionalities. These gels will be used to assess and compare diverse characterization techniques to establish a methodology to visualize gradients in multidimensional objects. In conclusion, the developed technology will be the first straightforward avenue to shaped hydrogels with functional and mechanical gradients. 5D Click Printing will be used to fabricate, bioinspired and sophisticated tissue models for biomedical application, and to produce graded membranes for chromatographic separation of complex biopolymer mixtures.
keywords Cellulose Nano-structured celluloses Gradient pronting Polyoxazolines Green chemistry
Publikationen
Project staff
Marco Beaumont
Dr. Marco Beaumont
marco.beaumont@boku.ac.at
Tel: +43 1 47654-77432, 77436
Project Leader
14.07.2020 - 24.01.2022
Thomas Rosenau
Univ.Prof. Dipl.-Chem. Dr.rer.nat. DDr.h.c. Thomas Rosenau
thomas.rosenau@boku.ac.at
Tel: +43 1 47654-77411, 77471
Project Leader
01.06.2020 - 13.07.2020
BOKU partners
External partners
University of Freiburg
none
partner
Queensland University of Technology (Brisbane, Australia)
none
partner
University of Vienna
none
partner