Development of a tag-less rNA downstream purification process and assistance in optimizing an affinity-based rNA downstream purification process

Development of a process to produce recombinant Influenza Neuraminidase (rNA) antigen in the baculovirus system, and especially downstream processing/purification will be performed in collaboration between the Icahn School of Medicine at Mount Sinai and the University of Natural Resources and Life Sciences to optimize an affinity purification-based downstream process for production of his-tagged rNA which can be successfully implemented at the CMO Expression Systems to produce enough rNA for a Collaborative Influenza Vaccine Innovation Centers (CIVICs) phase I clinical trial. Furthermore, to develop a high-yielding tag-less purification process that would allow us to get sufficient protein yields for post-phase I clinical development and lastly, to perform testing of alternative rNA expression constructs and expression systems. Doing this work will enable us to test rNA vaccines in clinical trials and may also provide a commercial path forward for rNA protein-based vaccine development in general.