Exact bacterial and cell population growth using holographic cytometry

When culturing any bacteria or cells, it is important to monitor the growth. The density of a culture determines, for example, its state and production yields. The current standard for measuring the growth of cultures is to measure the optical density (OD), in which the intensity of the light transmitted through the sample is compared to the intensity of the incident light. These measurements are often only taken at large time intervals and manually. They do not provide any information about the actual concentration of bacteria or cells. In this project, we will develop an automated system that provides users with the concentration of bacteria or cells during cultivation. The key enabling technology is high-throughput 3D microscopy, supported by precise automated dilution, integrated with the Hololoid holographic imaging and analytics platform.