Molecular biological characterization of surface layer glycan synthesis in Gram-positive bacteria. (P12966-MOB)
Abstract
Aneurinibacillus (formerly Bacillus) thermoaerophilus has been chosen for the investigations. The genetic experiments will be supported by biochemical studies on activated intermediates of the glycan biosynthesis pathway and topological characterisation of the glycosylation reactions by immunoelectron microscopy. From these experiments we expect insights into the molecular organization of the biosynthesis of S-layer glycoprotein glycans in Gram-positive bacteria. Further, the results should provide details for a possible genetic engineering of these glycosylated mac-romolecules to produce eventually taylored S-layer glycoproteins for specific biotechnological applications.
keywords S-layers prokaryotic glycoprotein genetic analysis Aneurinibacillus thermoaerophilus lipopolysaccharids biosynthesis
Publikationen
Genetische Charakterisierung von S-Schicht Glykoproteinen.
Autoren: Messner, P. Jahr: 1999
Forschungsbericht (extern. Auftraggeber)
Characterization of the S-layer glycoprotein synthesis of Aneurinibacillus thermoaerophilus.
Autoren: Kneidinger, B., Graninger, M., Messner, P. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
A novel linkage type and structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from <I>Aneurinibacillus thermoaerophilus</I> DSM 10155.
Autoren: Wugeditsch, T., Zachara, N. E., Puchberger, M., Kosma, P., Gooley, A. A., Messner, P. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Functional analysis of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase from Salmonella enterica LT2.
Autoren: Graninger, M., Nidetzky, B., Heinrichs, D. E., Whitfield, C., Palme, S., Zayni, S., Messner, P. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Purification, crystallization and preliminary structural studies of dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase (RmlC), the third enzyme of the dTDP-L-rhamnose synthesis pathway, from Salmonella enterica serovar typhimurium.
Autoren: Giraud, MF; Gordon, FM; Whitfield, C; Messner, P; McMahon, SA; Naismith, JH Jahr: 1999
Journal articles
Structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from Aneurinibacillus thermoaerophilus DSM 10155.
Autoren: Wugeditsch, T; Zachara, NE; Puchberger, M; Kosma, P; Gooley, AA; Messner, P Jahr: 1999
Journal articles
Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar Typhimurium LT2.
Autoren: Graninger, M; Nidetzky, B; Heinrichs, DE; Whitfield, C; Messner, P Jahr: 1999
Journal articles
Functional analysis of the rmlC and rmlD genes from Salmonella enterica serovar Typhimurium LT2, required for dTDP-L-rhamnose biosynthesis.
Autoren: Graninger, M., Kneidinger, B., Nidetzky, B., Whitfield, C., Messner, P. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Investigation of structure and antigenic capacities of Thermococcales cell envelopes and reclassification of "Caldococcus litoralis" Z-1301 as Thermococcus litoralis Z-1301.
Autoren: Kostyukova, AS; Gongadze, GM; Polosina, YY; Bonch-Osmolovskaya, EA; Miroshnichenko, ML; Chernyh, NA; Obraztsova, MV; Svetlichny, VA; Messner, P; Sleytr, UB; L'Haridon, S; Jeanthon, C; Prieur, D Jahr: 1999
Journal articles
Overexpression, purification, crystallization and preliminary structural study of dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD), the fourth enzyme of the dTDP-L-rhamnose synthesis pathway, from Salmonella enterica serovar Typhimurium.
Autoren: Giraud, MF; McMiken, HJ; Leonard, GA; Messner, P; Whitfield, C; Naismith, JH Jahr: 1999
Journal articles
The purification, crystallization and structural elucidation of dTDP-D-glucose 4,6-dehydratase (RmlB), the second enzyme of the dTDP-L-rhamnose synthesis pathway from Salmonella enterica serovar typhimurium.
Autoren: Allard, ST; Giraud, MF; Whitfield, C; Messner, P; Naismith, JH Jahr: 2000
Journal articles
Surface layer (S-layer) glycoproteins of Bacteria and Archaea.
Autoren: Messner, P., Schäffer, C. Jahr: 2000
Chapter in collected volumes
The role of the S-layer in facultatively alkaliphilic Bacillus pseudofirmus OF4.
Autoren: Gilmore, R., Guffanti, A., Messner, P., Kent, R., Krulwich, T.A. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
Genetische Charakterisierung von S-Schicht Glykoproteinen.
Autoren: Messner, P. Jahr: 2000
Forschungsbericht (extern. Auftraggeber)
A novel type of carbohydrate-protein linkage region in the tyrosine-bound S-layer glycan of Thermoanaerobacterium thermosaccharolyticum D120-70.
Autoren: Schäffer, C; Dietrich, K; Unger, B; Scheberl, A; Rainey, FA; Kählig, H; Messner, P Jahr: 2000
Journal articles
Two-dimensional gel electrophoresis analyses of pH-dependent protein expression in facultatively alkaliphilic Bacillus pseudofirmus OF4 lead to characterization of an S-layer protein with a role in alkaliphily.
Autoren: Gilmour, R; Messner, P; Guffanti, AA; Kent, R; Scheberl, A; Kendrick, N; Krulwich, TA Jahr: 2000
Journal articles
Genetische Charakterisierung von S-Schicht Glykoproteinen.
Autoren: Messner, P. Jahr: 2000
Forschungsbericht (extern. Auftraggeber)
Chromosome walking by cloning of distinct PCR fragments.
Autoren: Kneidinger, B; Graninger, M; Messner, P Jahr: 2001
Journal articles
Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T.
Autoren: Kneidinger, B; Graninger, M; Adam, G; Puchberger, M; Kosma, P; Zayni, S; Messner, P Jahr: 2001
Journal articles
Purification and structure elucidation of the N-acetylbacillosamine-containing polysaccharide from Bacillus licheniformis ATCC 9945.
Autoren: Schäffer, C; Scherf, T; Christian, R; Kosma, P; Zayni, S; Messner, P; Sharon, N Jahr: 2001
Journal articles
Prokaryotic glycosylation.
Autoren: Schäffer, C; Graninger, M; Messner, P Jahr: 2001
Journal articles
Cloning and functional characterization of the Pseudomonas aeruginosa rhlC gene that encodes rhamnosyltransferase 2, an enzyme responsible for di-rhamnolipid biosynthesis.
Autoren: Rahim, R; Ochsner, UA; Olvera, C; Graninger, M; Messner, P; Lam, JS; Soberón-Chávez, G Jahr: 2001
Journal articles
Biosynthesis of nucleotide-activated D-glycero-D-manno-heptose.
Autoren: Kneidinger, B; Graninger, M; Puchberger, M; Kosma, P; Messner, P Jahr: 2001
Journal articles
Molecular characterization of the glycosylation of S-layer glycoproteins from Gram-positive bacteria.
Autoren: Kneidinger, B., Schäffer, C., Graninger, M., Pföstl, A., Novotny, R., Schröfelbauer, B., Sleytr, U.B., Messner, P. Jahr: 2001
Conference & Workshop proceedings, paper, abstract
Biosynthesis of nucleotide-activated D-glycero-D-manno-heptose: a phosphatase as missing link.
Autoren: Kneidinger, B., Graninger, M., Messner, P. Jahr: 2001
Conference & Workshop proceedings, paper, abstract
Genetische Charakterisierung von S-Schicht Glykoproteinen.
Autoren: Messner, P. Jahr: 2001
Forschungsbericht (extern. Auftraggeber)
A model for the biosynthesis of the S-layer glycoprotein of Aneurinibacillus thermoaerophilus DSM 10155.
Autoren: Graninger, M., Kneidinger, B., Puchberger, M., Kosma, P., Schäffer, C., Messner, P. Jahr: 2001
Conference & Workshop proceedings, paper, abstract
Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli.
Autoren: Kneidinger, B; Marolda, C; Graninger, M; Zamyatina, A; McArthur, F; Kosma, P; Valvano, MA; Messner, P Jahr: 2002
Journal articles
The surface layer (S-layer) glycoprotein of Geobacillus stearothermophilus NRS 2004/3a. Analysis of its glycosylation.
Autoren: Schäffer, C; Wugeditsch, T; Kählig, H; Scheberl, A; Zayni, S; Messner, P; Jahr: 2002
Journal articles
Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155.
Autoren: Graninger, M; Kneidinger, B; Bruno, K; Scheberl, A; Messner, P Jahr: 2002
Journal articles
Functional expression of enterobacterial O-polysaccharide biosynthesis enzymes in Bacillus subtilis.
Autoren: Schäffer, C; Wugeditsch, T; Messner, P; Whitfield, C; Jahr: 2002
Journal articles
Chemical synthesis and biosynthetic pathways of nucleotide-activated heptoses
Autoren: Kneidinger, B., Zamyatina, A., Valvano, M., Messner, P., Kosma, P. Jahr: 2002
Conference & Workshop proceedings, paper, abstract
Prokaryotic Glycoproteins
Autoren: Schäffer, C., Messner, P. Jahr: 2003
Chapter in collected volumes
Purification and structure elucidation of the N-acetylbacillosamine- containing polysaccharide from Bacillus licheniformis ATCC 9945 using 800 MHz NMR spectroscopy
Autoren: Schaffer, C; Scherf, T; Christian, R; Kosma, P; Zayni, S; Messner, P; Sharon, N Jahr: 2000
Journal articles
Project staff
Paul Messner
Ao.Univ.Prof.i.R. Dipl.-Ing. Dr.nat.techn. Paul Messner
paul.messner@boku.ac.at
Project Leader
01.10.1998 - 30.09.2001
Christina Schäffer
Univ.Prof. Dipl.-Ing. Dr.nat.techn. Christina Schäffer
christina.schaeffer@boku.ac.at
Tel: +43 1 47654-80203
Project Staff
01.10.1998 - 30.09.2001
BOKU partners
External partners
University of Guelph
Prof. Dr. Chris Whitfield
partner