Studies on new microbial glycosyl transferases - trehalose phosphorylase from Schizophyllum commune.
Abstract
It is the objective of the project to characterize the enzyme trehalose phosphorylase from the basidiomycete Schizophyllum commune in detail, especially pertaining to the enzymology of the new phosphorylase and the biochemistry of the catalyzed reaction. The enzymic reaction engineering of trehalose synthesis is considered to be the biotechnical goal of this project. The biochemical part will deal with the molecular characterization of the enzyme, including (i) the cloning and sequencing of the gene encoding the trehalose phosphorylase, (ii) a kinetic characterization, and especially (iii) an extensive mechanistic characterization of the enzyme by using substrate analogues and inhibitors as specific probes. It is intended to elaborate in detail upon the differences in the glucosyl transferase mechanism employed by trehalose phosphorylase and a-D-glucan phosphorylases and other disaccharide phosphorylases.
keywords bonding energy catalysis enzyme reactor enzyme immobilization