K plus Competence Center Bio-Molecular Therapeutics BMT (Area 4)

01.06.2000 - 30.09.2005

Research project (§ 26 & § 27)

Program Kplus (Kompetenzzentren-Programm)

Funder Austrian Research Promotion Agency (FFG)

Competencies

Abstract

The research focus of the competence center ¿Bio-Molecular Therapeutics¿ (BMT) is applied research in the area of host defense in man. Its aim is to identify novel molecules, to functionally analyze these molecules, and to develop new methods for diagnostic and therapeutic strategies for diseases such as autoimmune diseases, allergies, and chronic inflammatory diseases, e. g. arteriosclerosis. The center is based on an initiative of institutes and clinics from the medical faculity of the University of Vienna, large Viennese pharmaceutical companies, and biotech-startups. The research program of the BMT is divided into four areas. The first area deals with the finding of novel genes in cells involved in host defense. The large number of identifed molecules will then be functionally analyzed in the second area. The third area is focused on the development of animal models for the characterized molecules. The fourth area serves to develop diagnostic and theapeutic tools as proof of concept for the importance of the molecules. The two projects in area four using crystalline bacterial cell surface (S-layer) proteins are focused on the production of highly ordered matrices as carriers for foreign proteins, peptides, polysaccharides or nucleic acids. This shall either be achieved by linking the foreign molecules to S-layer lattices or by recrystallization of functional S-layer fusion proteins on various types of supports and interfaces. The absolutely defined and repetitive surface properties of S-layer lattices down to the subnanometer scale will provide new solutions in diagnostics and for targeting and delivery systems.

Publications

Interaktionsstudien der zellassoziierten Exoamylase von Bacillus stearothermophilus ATCC 12980 mit N- oder C-terminal verkürzten Formen des S-Schicht-Proteins SbsC und peptidoglykanhältigen Zellwandfragmenten.

Autoren: Huber, C. Jahr: 2001

Master / Diploma Thesis

external links and characteristics of the publication:
Recombinant crystalline bacterial cell surface proteins: supramolecular arrays for nanobiotechnology.

Autoren: Breitwieser, A., Egelseer, E.M., Moll, D., Ilk, N., Neubauer, A., Sleytr, U.B., Sara, M. Jahr: 2001

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
S-layers and secondary cell wall polymers from Bacillus strains as building blocks for supramolecular engineering.

Autoren: Egelseer, E.M., Moll, D., Ilk, N., Breitwieser, A., Sleytr, U.B., Sára, M. Jahr: 2001

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:

www.scripps.edu
Recombinant S-layer-streptavidin fusion proteins: Functional protein lattices as nanoarrays for biochip development.

Autoren: Huber, C., Moll, D., Schlegel, B., Rünzler, D., Mader, C., Sára, M., Sleytr, U.B. Jahr: 2002

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
Molecular characterization of the S-layer gene sbpA of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen.

Autoren: Ilk, N., Völlenkle, C., Egelseer, E.M., Breitwieser, A., Sleytr, U.B., Sára, M. Jahr: 2002

Journal articles

external links and characteristics of the publication:
S-layer proteins as modules in supramolecular interfacial architectures

Autoren: Knoll, W., Sára, M., Pum, D., Sleytr, U.B. Jahr: 2002

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
S-layer fusion proteins and secondary cell wall polymers as building blocks in nanobiotechnology

Autoren: Egelseer, E.M., Sára, M. Jahr: 2002

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
S-layer-streptavidin fusion proteins as template for nanopatterned molecular arrays

Autoren: Moll, D., Huber, C., Schlegel, B., Pum, D., Sleytr, U.B., Sára, M. Jahr: 2002

Journal articles

external links and characteristics of the publication:

www.pnas.org
Biomimetic nanostructure fabrication: Non-lithographic lateral patterning and self-assembly of functional bacterial S-layers at silicon supports

Autoren: Györvary, E., O´Riordan, A., Quinn, A., Redmond, G., Pum, D., Sleytr, U.B. Jahr: 2003

Journal articles

external links and characteristics of the publication:

pubs.acs.org
Generation of a functional monomolecular protein lattice consisting of an s-layer fusion protein comprising the variable domain of a camel heavy chain antibody.

Autoren: Pleschberger, M; Neubauer, A; Egelseer, EM; Weigert, S; Lindner, B; Sleytr, UB; Muyldermans, S; Sára, M Jahr: 2003

Journal articles

external links and characteristics of the publication:

dx.doi.org
Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC

Autoren: Pavkov, T., Oberer, M., Egelseer, E.M., Sára, M., Sleytr, U.B., Keller, W. Jahr: 2003

Journal articles

external links and characteristics of the publication:
Biomolekulare selbst-assemblierende Systeme für die Nanobiotechnologie

Autoren: Pleschberger, M., Egelseer, E.M. Jahr: 2003

Newspaper / Magazine article

external links and characteristics of the publication:
S-Layers

Autoren: Sleytr, U.B., Egelseer, E.-M., Pum, D., Schuster, B. Jahr: 2004

Chapter in collected volumes

external links and characteristics of the publication:
Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification.

Autoren: Völlenkle, C; Weigert, S; Ilk, N; Egelseer, E; Weber, V; Loth, F; Falkenhagen, D; Sleytr, UB; Sára, M Jahr: 2004

Journal articles

external links and characteristics of the publication:
S-layer based nanostructures

Autoren: Pum, D., Sára, M., Schuster, B., Sleytr, U.B. Jahr: 2003

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
Interaction of the crystalline bacterial cell surface layer protein SbsB and the secondary cell wall polymer of Geobacillus stearothermophilus PV72 assessed by real-time surface plasmon resonance biosensor technology.

Autoren: Mader, C; Huber, C; Moll, D; Sleytr, UB; Sára, M Jahr: 2004

Journal articles

external links and characteristics of the publication:
Biophysical characterization of the entire bacterial surface layer protein SbsB and its two distinct functional domains.

Autoren: Rünzler, D; Huber, C; Moll, D; Köhler, G; Sára, M Jahr: 2004

Journal articles

external links and characteristics of the publication:

dx.doi.org
A functional chimaeric S-layer-enhanced green fluorescent protein to follow the uptake of S-layer-coated liposomes into eukaryotic cells.

Autoren: Ilk, N; Küpcü, S; Moncayo, G; Klimt, S; Ecker, RC; Hofer-Warbinek, R; Egelseer, EM; Sleytr, UB; Sára, M; Jahr: 2004

Journal articles

external links and characteristics of the publication:

dx.doi.org
An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology.

Autoren: Pleschberger, M; Saerens, D; Weigert, S; Sleytr, UB; Muyldermans, S; Sára, M; Egelseer, EM Jahr: 2004

Journal articles

external links and characteristics of the publication:

dx.doi.org
The three S-layer-like homology motifs of the S-layer protein SbpA of Bacillus sphaericus CCM 2177 are not sufficient for binding to the pyruvylated secondary cell wall polymer.

Autoren: Huber, C; Ilk, N; Rünzler, D; Egelseer, EM; Weigert, S; Sleytr, UB; Sára, M Jahr: 2005

Journal articles

external links and characteristics of the publication:

dx.doi.org
Genetically engineered S-layer proteins as nanopatterned biomaterials for applications in nanobiotechnology

Autoren: Huber, C. Jahr: 2004

Doctoral Thesis

external links and characteristics of the publication:
Micro- and nano-structured materials for extracorporeal blood purification

Autoren: Falkenhagen, D., Loth, F., Weber, V., Sara, M., Sleytr, U.B. Jahr: 2005

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
Nanobiotechnology technologies for extracorporeal bloodpurification systems – a new therapeutical approach in medicine

Autoren: Falkenhagen, D., Weber, V., Ettenauer, M., Sleytr, U.B., Sára, M. Jahr: 2005

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:

Project staff

Margit Sara

Project Leader

01.06.2000 - 30.09.2005

Eva Maria Egelseer

Dr. Eva Maria Egelseer

Project Staff

01.06.2000 - 30.09.2005

Carina Huber-Gries

Dipl.-Ing.Dr. Carina Huber-Gries

Project Staff

01.06.2000 - 30.09.2005

Uwe B. Sleytr

em.o.Univ.Prof. Dipl.-Ing.Dr. Uwe B. Sleytr
uwe.sleytr@boku.ac.at

Project Staff

01.06.2000 - 30.09.2005

BOKU partners

Institute of Nanobiotechnology (NBT)

role BOKU coordinator

External partners

Nanosearch Membrane GmbH

Dr. Wolfgang Schallenberger

partner

BMT GmbH

Gudrun Baumann

coordinator

Baxter AG

Birgit Reipert

partner

Biomax Informatics AG

Dr. Zollner

partner

Novartis Forschungsinstitut GmbH

Prof. Jan E. de Vries

partner

Technoclone GesmbH

Prof. Bernd Binder

partner

Polymun Scientific Immunologie ForschungsgesmbH.

Prof. Hermann Katinger

partner

University of Vienna, Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases

Prof. Georg Stingl

partner

University of Vienna, Institute of Immunology

Prof. Walter Knapp

partner

University of Vienna, Institute of Vascular Biology and Thrombosis Research

Prof. Bernd Binder

partner

K plus Competence Center Bio-Molecular Therapeutics BMT (Area 4)

Abstract

Publications

Interaktionsstudien der zellassoziierten Exoamylase von Bacillus stearothermophilus ATCC 12980 mit N- oder C-terminal verkürzten Formen des S-Schicht-Proteins SbsC und peptidoglykanhältigen Zellwandfragmenten.

Recombinant crystalline bacterial cell surface proteins: supramolecular arrays for nanobiotechnology.

S-layers and secondary cell wall polymers from Bacillus strains as building blocks for supramolecular engineering.

Recombinant S-layer-streptavidin fusion proteins: Functional protein lattices as nanoarrays for biochip development.

Molecular characterization of the S-layer gene sbpA of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen.

S-layer proteins as modules in supramolecular interfacial architectures

S-layer fusion proteins and secondary cell wall polymers as building blocks in nanobiotechnology

S-layer-streptavidin fusion proteins as template for nanopatterned molecular arrays

Biomimetic nanostructure fabrication: Non-lithographic lateral patterning and self-assembly of functional bacterial S-layers at silicon supports

Generation of a functional monomolecular protein lattice consisting of an s-layer fusion protein comprising the variable domain of a camel heavy chain antibody.

Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC

Biomolekulare selbst-assemblierende Systeme für die Nanobiotechnologie

S-Layers

Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification.

S-layer based nanostructures

Interaction of the crystalline bacterial cell surface layer protein SbsB and the secondary cell wall polymer of Geobacillus stearothermophilus PV72 assessed by real-time surface plasmon resonance biosensor technology.

Biophysical characterization of the entire bacterial surface layer protein SbsB and its two distinct functional domains.

A functional chimaeric S-layer-enhanced green fluorescent protein to follow the uptake of S-layer-coated liposomes into eukaryotic cells.

An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology.

The three S-layer-like homology motifs of the S-layer protein SbpA of Bacillus sphaericus CCM 2177 are not sufficient for binding to the pyruvylated secondary cell wall polymer.

Genetically engineered S-layer proteins as nanopatterned biomaterials for applications in nanobiotechnology

Micro- and nano-structured materials for extracorporeal blood purification

Nanobiotechnology technologies for extracorporeal bloodpurification systems – a new therapeutical approach in medicine

Project staff

Margit Sara

Eva Maria Egelseer

Carina Huber-Gries

Uwe B. Sleytr

BOKU partners

Institute of Nanobiotechnology (NBT)

External partners

Nanosearch Membrane GmbH

BMT GmbH

Baxter AG

Biomax Informatics AG

Novartis Forschungsinstitut GmbH

Technoclone GesmbH

Polymun Scientific Immunologie ForschungsgesmbH.

University of Vienna, Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases

University of Vienna, Institute of Immunology

University of Vienna, Institute of Vascular Biology and Thrombosis Research

Funder

Austrian Research Promotion Agency, Division 2: Science/Industry Cooperation

Vienna Chamber of Commerce and Industry