Screening, chemical characterization and biosynthesis of S-layers. (S7201-MOB)
Abstract
The cell surface of many archaebacteria and eubacteria is covered with a crystalline, monomolecular layer (S-layer) which is assembled of identical protein monomers. Recently, within the eubacteria, glycosylated S-layer proteins have been found. Aim of the project is to determine the structure of the carbohydrate chains as well as the protein-carbohydrate linkage regions. Additionally, biosynthesis studies of eubacterial S-layer glycoproteins should be performed.
Publications
Accurate determination of the molecular weight of the major surface layer protein isolated from Clostridium thermosaccharolyticum by time-of-flight mass spectrometry.
Autoren: Allmaier, G; Schäffer, C; Messner, P; Rapp, U; Mayer-Posner, FJ Jahr: 1995
Journal articles
Glycan structure of the S-layer glycoprotein of Bacillus sp. L420-91.
Autoren: Kosma, P; Neuninger, C; Christian, R; Schulz, G; Messner, P Jahr: 1995
Journal articles
Are S-layer glycoproteins and lipopolysaccharides related?
Autoren: Schäffer, C; Wugeditsch, T; Neuninger, C; Messner, P Jahr: 1996
Journal articles
Synthesis of GDP-2-acetamido-2-deoxy-alpha-D-mannose (GDP-ManNAc).
Autoren: Klaps, E; Neuninger, C; Messner, P; Schmid, W Jahr: 1996
Journal articles
external links and characteristics of the publication:Desulfurobacterium thermolithotrophum gen. nov., sp. nov., a novel autotrophic, sulphur-reducing bacterium isolated from a deep-sea hydrothermal vent.
Autoren: L'Haridon, S; Cilia, V; Messner, P; Ragúenès, G; Gambacorta, A; Sleytr, UB; Prieur, D; Jeanthon, C Jahr: 1998
Journal articles
Characterization of enzymes involved in S-layer glycoprotein glycan biosynthesis.
Autoren: Messner, P., Graninger, M., Schäffer, C., Wugeditsch, T., Whitfield, C. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Isolation and characterization of an amino sugar-rich glycopeptide from the surface layer glycoprotein of Thermoanaerobacterium thermosaccharolyticum E207-71.
Autoren: Altman, E; Schäffer, C; Brisson, JR; Messner, P Jahr: 1996
Journal articles
Synthesis of 5-azidomethyl-2'-deoxyuridine-5'-triphosphate as a photoaffinity probe.
Autoren: Klaps, E., Zöllner, P., Graninger, M., Messner, P., Schmid, W. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Crystalline bacterial cell surface layers (S-layers).
Autoren: Wugeditsch, T., Messner, P. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Eubacterial glycosylated S-layer proteins: architectural and biosynthetic aspects.
Autoren: Schäffer, C., Messner, P. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Eubacterial S-layer glycoproteins.
Autoren: Wugeditsch, T., Schäffer, C., Graninger, M., Messner, P. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:How diverse are glycans of eubacterial S-layer glycoproteins?
Autoren: Schäffer, C., Neuninger, C., Wugeditsch, T., Messner, P. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Activation of rhamnose for incorporation in S-layer glycoproteins of different thermophilic Bacillaceae.
Autoren: Graninger, M., Schäffer, C., Wugeditsch, T., Messner, P. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Screening, chemische Charakterisierung und Biosynthese von S-Schichten.
Autoren: Messner, P. Jahr: 1999
Forschungsbericht (extern. Auftraggeber)
external links and characteristics of the publication:Kristalline Zelloberflächen-Schichten prokaryotischer Organismen (S-Schichten): von der supramolekularen Zellstruktur zur Biomimetik und Nanotechnologie.
Autoren: Sleytr, U B; Messner, P; Pum, D; Sara, M Jahr: 1999
Journal articles
external links and characteristics of the publication:Screening, chemische Charakterisierung und Biosynthese von S-Schichten.
Autoren: Messner, P. Jahr: 1999
Forschungsbericht (extern. Auftraggeber)
external links and characteristics of the publication:Complete glycan structure of the S-layer glycoprotein of Aneurinibacillus thermoaerophilus GS4-97.
Autoren: Schäffer, C; Müller, N; Christian, R; Graninger, M; Wugeditsch, T; Scheberl, A; Messner, P Jahr: 1999
Journal articles
Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar Typhimurium LT2.
Autoren: Graninger, M; Nidetzky, B; Heinrichs, DE; Whitfield, C; Messner, P Jahr: 1999
Journal articles
Surface layer (S-layer) glycoproteins of Bacteria and Archaea.
Autoren: Messner, P., Schäffer, C. Jahr: 2000
Chapter in collected volumes
external links and characteristics of the publication:Structure of a novel heptose-containing eubacterial S-layer glycoprotein glycan.
Autoren: Wugeditsch, T., Kosma, P., Christian, R., Messner, P. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Glycan structure of a heptose-containing S-layer glycoprotein.
Autoren: Wugeditsch, T., Kosma, P., Christian, R., Messner, P. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Similarity of "core" structures in two different glycans of tyrosine-linked eubacterial S-layer glycoproteins.
Autoren: Messner, P; Christian, R; Neuninger, C; Schulz, G Jahr: 1995
Journal articles
Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207-71.
Autoren: Altman, E; Schäffer, C; Brisson, JR; Messner, P Jahr: 1995
Journal articles
Occurrence, location, ultrastructure and morphogenesis of S-layers.
Autoren: Sleytr, U. B., Messner, P., Pum, D., Sara, M. Jahr: 1996
Chapter in collected volumes
external links and characteristics of the publication:Chemical composition and biosynthesis of S-layers.
Autoren: Messner, P. Jahr: 1996
Chapter in collected volumes
external links and characteristics of the publication:Description of Bacillus thermoaerophilus sp. nov., to include sugar beet isolates and Bacillus brevis ATCC 12990.
Autoren: Meier-Stauffer, K., Busse, H.-J., Rainey, F. A., Burghardt, J., Scheberl, A., Hollaus, F., Kuen, B., Makristathis, A., Sleytr, U. B., Messner, P. Jahr: 1996
Journal articles
Anaerofilum pentosovorans gen. nov., sp. nov., and Anaerofilum agile sp. nov., two new, strictly anaerobic, mesophilic, acidogenic bacteria from anaerobic bioreactors.
Autoren: Zellner, G; Stackebrandt, E; Nagel, D; Messner, P; Weiss, N; Winter, J Jahr: 1996
Journal articles
Thermobrachium celere gen. nov., sp. nov., a rapidly growing thermophilic, alkalitolerant, and proteolytic obligate anaerobe.
Autoren: Engle, M; Li, Y; Rainey, F; DeBlois, S; Mai, V; Reichert, A; Mayer, F; Messner, P; Wiegel, J Jahr: 1996
Journal articles
Crystalline bacterial cell surface proteins. Molecular Biology Intelligence Unit.
Autoren: Sleytr, U. B., Messner, P., Pum, D., Sara, M. (eds.) Jahr: 1996
Editorial
external links and characteristics of the publication:Methanococcus infernus sp. nov., a novel hyperthermophilic lithotrophic methanogen isolated from a deep-sea hydrothermal vent.
Autoren: Jeanthon, C; L'Haridon, S; Reysenbach, AL; Vernet, M; Messner, P; Sleytr, UB; Prieur, D Jahr: 1998
Journal articles
S-layered Aneurinibacillus and Bacillus spp. are susceptible to the lytic action of Pseudomonas aeruginosa membrane vesicles.
Autoren: Kadurugamuwa, JL; Mayer, A; Messner, P; Sára, M; Sleytr, UB; Beveridge, TJ Jahr: 1998
Journal articles
Methanoculleus palmolei sp. nov., an irregularly coccoid methanogen from an anaerobic digester treating wastewater of a palm oil plant in north-Sumatra, Indonesia.
Autoren: Zellner, G; Messner, P; Winter, J; Stackebrandt, E Jahr: 1998
Journal articles
Overexpression, purification, and characterization of enzymes involved in the biosynthesis of TDP-L-rhamnose.
Autoren: Graninger, M., Messner, P., Heinrichs, D. E., Whitfield, C. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Distinction between S(surface)-layer glycoproteins and accessory cell wall polymers of Bacillaceae.
Autoren: Schäffer, C., Kählig, H., Christian, R., Scheberl, A., Zayni, S., Messner, P. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:NMR investigations of bacterial cell wall polysaccharides from Bacillus stearothermophilus.
Autoren: Schäffer, C., Kählig, H., Messner, P. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Nitritbildung im Dünnsaft durch Thermus-Arten.
Autoren: Hollaus, F., Hein, W., Pollach, G., Scheberl, A., Messner, P. Jahr: 1997
Newspaper / Magazine article
external links and characteristics of the publication:Biochemistry of S-layers.
Autoren: Messner, P; Allmaier, G; Schäffer, C; Wugeditsch, T; Lortal, S; König, H; Niemetz, R; Dorner, M Jahr: 1997
Journal articles
Taxonomic comparison of different thermophilic sugar beet isolates with glycosylated surface layer (S-layer) proteins and their affiliation to Bacillus smithii.
Autoren: Messner, P., Scheberl, A., Schweigkofler, W., Hollaus, F., Rainey, F. A., Burghardt, J., Prillinger, H.-J. Jahr: 1997
Journal articles
Characterization of glycosylation enzymes from thermophilic Bacillaceae.
Autoren: Graninger, M., Schäffer, C., Wugeditsch, T., Zayni, S., Scheberl, A., Messner, P. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:TDP-rhamnose biosynthesis in different thermophilic Bacillaceae producing rhamnose-containing S-layer glycoproteins.
Autoren: Graninger, M., Wugeditsch, T., Schäffer, C., Messner, P. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Two S-layered Bacillus spp. are susceptible to the lytic action of Pseudomonas aeruginosa membrane vesicles.
Autoren: Kadurugamuwa, J., Messner, P., Beveridge, T. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:The surface layer (S-layer) glycoproteins of bacteria.
Autoren: Messner, P., Schäffer, C., Wugeditsch, T., Graninger, M. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Characterization of the heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus.
Autoren: Wugeditsch, T., Kosma, P., Schäffer, C., Messner, P. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Contributions of mass spectrometry to microbial S-layer research.
Autoren: Allmaier, G., Dorner, M., Messner, P. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Synthesis of GDP-2-acetamido-2-deoxy-alpha-D-mannose (GDP-ManNAc) and its phosphonate analogue.
Autoren: Klaps, E., Cipolla, L., Messner, P., Neuninger, C., Nicotra, F., Schmid, W. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Description of an amino sugar-rich glycan structure from the surface layer glycoprotein of Thermoanaerobacterium thermosaccharolyticum E207-71.
Autoren: Schäffer, C., Altman, E., Brisson, J.-R., Messner, P. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Glycosylation of S-layer glycoproteins from thermophilic eubacteria.
Autoren: Schäffer, C., Wugeditsch, T., Messner, P. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Complete glycan structure of the heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus.
Autoren: Wugeditsch, T., Kosma, P., Christian, R., Messner, P. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Mass spectrometric (MALDI, PD and ESI) techniques applied to the analysis of glycan structures from crystalline S-layer glycoproteins of thermophilic eubacteria.
Autoren: Allmaier, G., Messner, P. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Synthesis of GDP-2-acetamido-2-deoxy-alpha-D-mannose (GDP-ManNAc).
Autoren: Klaps, E., Neuninger, C., Messner, P., Schmid, W. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Are S-layer glycoproteins and lipopolysaccharides related?
Autoren: Messner, P., Schäffer, C., Neuninger, C., Wugeditsch, T. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Two-dimensional protein crystals: fundamentals and applications for biomimetic membranes and molecular nanotechnology.
Autoren: Sleytr, U. B., Sara, M., Pum, D., Messner, P., Küpcü, S. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Glycosylated S(surface)-layer proteins have been established as constituents of archaeobacterial as well as of eubacterial cell envelopes.
Autoren: Schäffer, C., Wugeditsch, T., Graninger, M., Messner, P. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Complete glycan structure of the S-layer glycan of <I>Aneurinibacillus thermoaerophilus</I> GS4-97.
Autoren: Schäffer, C., Müller, N., Christian, R., Graninger, M., Wugeditsch, T., Scheberl, A., Messner, P. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Screening, chemische Charakterisierung und Biosynthese von S-Schichten.
Autoren: Messner, P. Jahr: 1998
Forschungsbericht (extern. Auftraggeber)
external links and characteristics of the publication:Crystalline bacterial cell surface layers (S-layers): from supramolecular cell structure to biomimetics and nanotechnology.
Autoren: Sleytr, U. B., Messner, P., Pum, D., Sara, M. Jahr: 1999
Journal articles
A novel linkage type and structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from <I>Aneurinibacillus thermoaerophilus</I> DSM 10155.
Autoren: Wugeditsch, T., Zachara, N. E., Puchberger, M., Kosma, P., Gooley, A. A., Messner, P. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Functional analysis of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase from Salmonella enterica LT2.
Autoren: Graninger, M., Nidetzky, B., Heinrichs, D. E., Whitfield, C., Palme, S., Zayni, S., Messner, P. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
external links and characteristics of the publication:Thermococcus barophilus sp. nov., a new barophilic and hyperthermophilic archaeon isolated under high hydrostatic pressure from a deep-sea hydrothermal vent.
Autoren: Marteinsson, VT; Birrien, JL; Reysenbach, AL; Vernet, M; Marie, D; Gambacorta, A; Messner, P; Sleytr, UB; Prieur, D Jahr: 1999
Journal articles
Methanococcus vulcanius sp. nov., a novel hyperthermophilic methanogen isolated from East Pacific Rise, and identification of Methanococcus sp. DSM 4213T as Methanococcus fervens sp. nov.
Autoren: Jeanthon, C; L'Haridon, S; Reysenbach, AL; Corre, E; Vernet, M; Messner, P; Sleytr, UB; Prieur, D Jahr: 1999
Journal articles
Purification, crystallization and preliminary structural studies of dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase (RmlC), the third enzyme of the dTDP-L-rhamnose synthesis pathway, from Salmonella enterica serovar typhimurium.
Autoren: Giraud, MF; Gordon, FM; Whitfield, C; Messner, P; McMahon, SA; Naismith, JH Jahr: 1999
Journal articles
The diacetamidodideoxyuronic-acid-containing glycan chain of Bacillus stearothermophilus NRS 2004/3a represents the secondary cell-wall polymer of wild-type B. stearothermophilus strains.
Autoren: Schäffer, C; Kählig, H; Christian, R; Schulz, G; Zayni, S; Messner, P Jahr: 1999
Journal articles
Structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from Aneurinibacillus thermoaerophilus DSM 10155.
Autoren: Wugeditsch, T; Zachara, NE; Puchberger, M; Kosma, P; Gooley, AA; Messner, P Jahr: 1999
Journal articles
Crystalline bacterial cell surface layers (S-layers).
Autoren: Sleytr, U.B., Messner, P. Jahr: 2000
Chapter in collected volumes
external links and characteristics of the publication:
Project staff
Paul Messner
Ao.Univ.-Prof.i.R. Dipl.-Ing.Dr.nat.techn. Paul Messner
paul.messner@boku.ac.at
Project Leader
01.04.1994 - 31.05.1999
Christina Schäffer
Univ.Prof. Dipl.-Ing.Dr.nat.techn. Christina Schäffer
christina.schaeffer@boku.ac.at
Tel: +43 1 47654-80203
Project Staff
01.04.1994 - 31.05.1999
BOKU partners
External partners
National Research Council of Canada, Institute for Biological Sciences
Dr. Eleonora Altman
partner
Scientific Software Company
Rudolf Christian
partner
Macquarie University
Prof. Dr. Andrew A. Gooley
partner
University Linz, Institute for Chemistry
Prof. Dr. Norbert Müller
partner
University of Vienna, Institute of Organic Chemistry
Dr. Hanspeter Kählig
partner
University of Vienna, Institute of Analytical Chemistry
Prof. Dr. Günter Allmaier
partner

jb.asm.org