Bacterial cell surface display of "humanized" oligosaccharides (NESTEC)
Abstract
Glycoconjugates play a crucial role in many of the recognition, signalling, and adhesion events that take place at the surface of cells, with the oligosaccharide structures being the key to their functions. Cell-surface display of rationally designed glycosylation motifs is generally considered a versatile tool for applied research, leading to the expression of various antigentic determinants, tissue-targeting signals, or receptor mimics. Surface-layer (S-layer) covered Bacillaceae and lactic acid bacteria are promising candidates for a combined cell surface display-carbohydrate engineering approach. Besides regularity and multivalency of the S-layer lattices, these organisms offer certain advantages of Gram-positive expression systems In the current project, S-layers shall be exploited for presentation of "humanized oligosaccharides" in the course of the development of next generation functional food and drug applications. Well-characterized enzymes from the exopolysaccharide biosynthesis pathway of lactic acid bacteria shall be used for the generation of tailor-made S-layer neoglycoconjugates.
dx.doi.orgProject staff
Christina Schäffer
Univ.Prof. Dipl.-Ing.Dr.nat.techn. Christina Schäffer
christina.schaeffer@boku.ac.at
Tel: +43 1 47654-80203
Project Leader
01.05.2002 - 31.08.2006
Paul Messner
Ao.Univ.-Prof.i.R. Dipl.-Ing.Dr.nat.techn. Paul Messner
paul.messner@boku.ac.at
Project Staff
01.05.2002 - 31.08.2006
BOKU partners
External partners
Algerian Institute for Petrol
partner
Funder
