Assessing the potential of isotopes and elements in different hard parts of freshwater fish species to determine origin and migratory patterns using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
Abstract
Chemical environmental information is to a certain degree stored in soft and hard tissues of fish and other living organisms, although being influenced by physiological processes. Consequently site- and species specific microchemical patterns stored in hard parts of fish can be used as natural chemical tags for studying the spatial behaviour of fish for answering important ecological questions like the origin of fish, stock identification or dispersal and other migratory patterns (homing of fish to natal birthplaces, individual and group life histories or habitat use). Although mainly otoliths have been studied so far, scales and fin rays bare the potential for non-lethal sampling, especially needed when studying endangered species. On the other hand eye lenses could be sampled easily, which is of special relevance when large numbers of fish are investigated. Therefore the “IsoMark” project focuses for the first time on the comprehensive investigation of microchemical information (elemental fingerprint of Ca, Sr, Na, Ba, Mg; isotopic fingerprint of Sr and Ca) in different hard parts of typical European freshwater fish species like brown trout (Salmo trutta f.f., L.), European grayling (Thymallus thymallus, L.), nase (Chondrostoma nasus, L.) and the Danube salmon (Hucho hucho, L.). Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) will be used as major technique for direct and simultaneous in situ high precision analysis of trace elements and isotope ratios of hard parts. Fish from ten fish farms will be analyzed for their microchemical signatures in different hard parts as fish farm specific signatures would make expensive artificial tagging, e.g. to track farmed fish in natural rivers after stocking, nonessential. As a reliable interpretation of the chemical patterns in different hard parts requires an understanding of the relationship between environmental conditions and the uptake of elements and isotopes, controlled field experiments will be conducted for two species (brown trout and nase) at different life stages. Additionally adult and juvenile individuals of brown trout and nase from natural rivers with different environmental chemistry will be sampled to assess the influence of geological formations, ontogeny and temperature on microchemical patterns in hard parts. The stability of the chemical fingerprints of rivers as a pre-requisite for a clear interpretation of microchemical patterns in hard parts will be assessed as well. Trans-generational marking via injecting enriched isotopes into gravid females will be tested offering an amazing opportunity to mark large numbers of larvae in the field since the environmental information experienced by adult females is passed over to the primordial core of the otolith. The project will be run as collaboration between the Division of Analytical Chemistry (Department of Chemistry) and the Institute of Hydrobiology and Aquatic Ecosystem Management (Department of Water, Atmosphere & Environment) at the BOKU, University of Natural Resources and Applied Life Sciences, Vienna, together with the Institute for Water Ecology, Fisheries and Lake Research at Mondsee/Scharfling (Federal Agency for Water Management), and is directly related to the research of the START-project “VIRIS” (Vienna Isotope Research Investigation and Survey, FWF 267-N11).
keywords fish migration isotope analysis fish stock management
Publikationen
Natürliche Isotopenmuster in Hartteilen von Fischen erlauben die Untersuchung von Herkunft und Wanderungen - Start des ISOMARK Projektes
Autoren: Zitek, A, Prohaska, T; Irrgeher, J; Jagsch, A; Waidbacher, H Jahr: 2009
Journal articles
WFD and connectivity problems: solutions at the European scale
Autoren: Zitek, A. Jahr: 2009
Conference & Workshop proceedings, paper, abstract
IsoMark - a comprehensive assessment of the potential of isotopes in hard parts of freshwater fish to determine origin and migratory patterns using LA-(MC)-ICP-MS
Autoren: Zitek, A., Irrgeher, J., Sturm, M., Brunner, M., Dillinger, B., Prohaska, T. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
IsoMark - a comprehensive assessment of the potential of isotopes in hard parts of freshwater fish to determine origin and migratory patterns using LA-(MC)-ICP-MS
Autoren: Zitek, A., Irrgeher, J., Sturm, M., Brunner, M., Sailer, K., Altmann, M., Dillinger, B.,Waidbacher, H. and T. Prohaska Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Discrimination of wild and hatchery trout by natural chronological patterns of elements and isotopes in otoliths using LA-ICP-MS
Autoren: Zitek, A; Sturm, M; Waidbacher, H; Prohaska, T Jahr: 2010
Journal articles
The use of strontium isotopes as indicator of migration and mobility
Autoren: Irrgeher, J., Zitek, A., Teschler-Nicola, M., Prohaska, T, Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Anwendung von Strontium - Isotopensignaturen für Mobilitäts- und Migrationsstudien
Autoren: Irrgeher, J., Zitek, A., Teschler-Nicola, M., Prohaska, T. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Anwendung von Strontium - Isotopensignaturen für Mobilitäts- und Migrationsstudien
Autoren: Irrgeher, J; Zitek, A; Teschler-Nicola, M;Prohaska, T Jahr: 2010
Journal articles
Sr – an element shows the way – Applications of Sr isotopes for provenance, tracing and migration
Autoren: Prohaska, T., Irrgeher, J., Zitek, A., Teschler-Nicola, M. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Tracing and spiking
Autoren: Prohaska, T., Irrgeher, J., Zitek, A., Walczyck, T., Schulze, T., Teschler-Nicola, M. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Isoscapes - a powerful tool for the management of large river systems
Autoren: Zitek, A., Irrgeher, J., Sailer, K., Trautwein, C., Kralik, M., Waidbacher, H., Hein, T., Prohaska, T. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Transgenerational marking of a freshwater fish species, Salmo trutta f.f., using an enriched 84Sr spike solution
Autoren: Zitek, A., Irrgeher, J., Jagsch, A., Kletzl, M., Weisman, T., Prohaska, T. Jahr: 2011
Journal articles
The potential of microchemical information in fish hard parts to study origin and migration in the Austrian Danube catchment
Autoren: Sailer, K., Zitek, A., Waidbacher, H., Irrgeher, J., Prohaska, T. Jahr: 2011
Journal articles
Microchemical analysis of 48 water samples from 16 sites at Bavarian lakes and river sections
Autoren: Irrgeher, J., Theiner, S., Prohaska, T., Zitek, A. Jahr: 2011
Forschungsbericht (extern. Auftraggeber)
Erste Vorergebnisse zur Mikrochemie von Saiblings-Otolithen unbekannter Herkunft aus Aquakultur
Autoren: Irrgeher, J., Prohaska, T., Zitek, A. Jahr: 2011
Forschungsbericht (extern. Auftraggeber)
Isopscapes - a powerful tool for the management of large river systems
Autoren: Zitek, A., Irrgeher, J., Sailer, K., Trautwein, C., Kralik, M., Waidbacher, H., Hein, T., Prohaska, T. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
The use of strontium isotopes as indicator of migration and mobility
Autoren: Irrgeher, J., Zitek, A., Teschler-Nicola, M., Prohaska, T. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Revealing feeding grounds of cormorants by analysing their prey remains
Autoren: Oehm, J., Thalinger, B., Traugott, M., Zitek, A., Prohaska, T. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Herkunftskontrolle von Fischen aus Aquakultur mittels Element- und Isotopenanalytik - Kurzzusammenfassung einer Pilot-Studie zur Verwendung der zeitaufgelösten Analyse der Otolithen(Gehörstein)-Chemie mittels Laser-Ablations-Induktiv-Gekoppelter-Plasma-Massenspektrometrie für die Herkunftskontrolle bei Fischen aus ausgewählten Alpenlachs®-Zuchten
Autoren: Zitek, A., Irrgeher, J., Prohaska, T. Jahr: 2012
Forschungsbericht (extern. Auftraggeber)
Herkunftskontrolle von Fischen aus Aquakultur mittels Element- und Isotopenanalytik: Eine Pilot-Studie zur Verwendung der zeitaufgelösten Analyse der Otolithen(Gehörstein)-Chemie mittels Laser-Ablations-Induktiv-Gekoppelter-Plasma-Massenspektrometrie (LA-ICPMS) für die Herkunftskontrolle bei Fischen aus den Alpenlachs®- Zuchten Grundlsee, Xeis und Taxacher
Autoren: Zitek, A., Irrgeher, J., Prohaska, T. Jahr: 2012
Forschungsbericht (extern. Auftraggeber)
Chemical imaging of isotopic spikes in hard tissues using LA-ICPMS
Autoren: Prohaska, T., Horsky, M., Irrgeher, J., Zitek, A., Teschler-Nicola, M., Schulze-Koenig, T., Walcyk, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Sr isotopic investigations of calcium-rich matrices by LA-MC-ICPMS
Autoren: Irrgeher, J., Galler, P., Zitek, A., Teschler-Nicola, M., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Anwendungsmöglichkeiten einer 87Sr/86Sr Isoscape auf fischökologische Fragestellungen im Donaueinzugsgebiet
Autoren: Zitek, A., Irrgeher, J., Sailer, K., Trautwein, C., Waidbacher, H., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Sr-Isotopenverhältnis-Messungen in calciumreichen Probenmatrizes mittels LA-MC-ICPMS
Autoren: Irrgeher, J., Zitek, A., Teschler-Nicola, M., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Transgenerationales Markieren von Karpfen (Cyprinus carpio) mittels eines Strontium-Isotopen -Doppelspikes
Autoren: Cervicek, M., Zitek, A., Irrgeher, J., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Herkunftsbestimmung von Fischen aus Flusssystemen mit Hilfe von Element- und Isotopenbestimmung Gehörsteinen (Otolithen)
Autoren: Kendlbacher, F., Zitek, A., Waidbacher, H., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Isotopic and elemental composition of different hard parts of fish and their ecogeochemical application
Autoren: Zitek, A., Irrgeher, J., Huber, R., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Stable Sr isotope fractionation in biological and geological samples from an Alpine biosphere reserve: effects of metabolic processes and weathering
Autoren: Irrgeher, J., Zitek, A., You, C.-F., Chung, C.-H., Liu, H.-C., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Wo, woher oder wohin? Einsatz der modernen Isotopenanalytik zur Herkunftsbestimmung von Lebensmitteln
Autoren: Irrgeher, J. Zitek, A., Prohaska, T. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Herkunftsbestimmung bei Fisch mittels Isotopenfingerabdruck
Autoren: Zitek, A., Irrgeher, J., Prohaska, T. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Sr isotope ratios in calcium-rich matrices by (LA)-MC-ICPMS: An ongoing challenge
Autoren: Irrgeher, J., Galler, P., Zitek, A., Teschler-Nicola, M. and Prohaska, T. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
The potential of isotopic research in analytical ecogeochemistry
Autoren: Prohaska, T., Irrgeher, J., Kappel, S., Horsky, M., Koffler , D., Laaha, G., Leisch, F. and Zitek, A. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Altersbestimmung von Aalen aus dem Eglsee, Ausrinn des Seeoner Sees anhand von geschliffenen Otolithen
Autoren: Zitek, A. Jahr: 2013
Forschungsbericht (extern. Auftraggeber)
Transgenerational isotopic marking of carp Cyprinus carpio, L. using a 86Sr /84Sr double spike
Autoren: Zitek, A., Cervicek, M., Irrgeher, J., Horsky, M., Kletzl, M., Weismann, T., Prohaska, T. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
The potential of isoscapes and isozones of non traditional isotopes in analytical ecogeochemistry
Autoren: Prohaska, T. Irrgeher, J., Horsky, M., Zitek, A. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Comparison of the elemental composition of different hard parts (otoliths, scales, fin rays, vertebrae and eye lenses) of freshwater fish using ICPMS
Autoren: Kirchmaier, L, Irrgeher, J., Prohaska, T., Zitek, A. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Herkunftsbestimmung von Eismeersaiblingen anhand des 87Sr/86Sr Verhältnisses im Otolithenkern mittels Laser Abtragungs-Multi Kollektor-Induktiv Gekoppelter Plasma Massenspektrometrie: Eier aus Schweden oder Island?
Autoren: Zitek, A., Irrgeher, J., Prohaska, T. Jahr: 2013
Expertengutachten (extern. Auftraggeber)
Transgenerational isotopic marking of carp Cyprinus carpio L. using a 86Sr/84Sr double spike
Autoren: Zitek, A., Irrgeher, J., Horsky, M., Kletzl, M., Weismann, T., Prohaska, T. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
From ecology to fish pass design and monitoring to population modelling for sturgeons in the lower Danube
Autoren: Zitek, A. Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Project staff
Thomas Prohaska
Ao.Univ.Prof. Dipl.-Ing. Dr.techn. Thomas Prohaska
thomas.prohaska@boku.ac.at
Project Leader
01.10.2009 - 31.03.2013
Andreas Zitek
Dipl.-Ing. Dr.nat.techn. Andreas Zitek MSc.
andreas.zitek@boku.ac.at
Tel: +43 1 47654-31212
Sub Projectleader
01.10.2009 - 31.03.2013
BOKU partners
External partners
Bundesamt für Wasserwirtschaft, Institut für Gewässerökologie
HR Dr. Albert Jagsch
partner