Glycogenomics of the opportunistic fungal pathogen Aspergillus fumigatus
- Lebensmittel, Ernährung, Gesundheit
- Biotechnologie
- Forschungscluster "Bioindustrielle Technologien"
Abstract
Aspergillus fumigatus is the most common opportunistic fungal pathogen of humans, causing fatal invasive aspergillosis (IA) in immuno-compromised patients. In the proposed project, three putative glycoenzyme genes, mnn9, gpf1 and ncr, have been chosen for further study. All three are predicted to have different roles in enabling the modification of the outer chains of N- or O-glycans. These genes will be cloned and expressed recombinantly in Pichia as well as be knocked-out in Aspergillus. The activity of the enzymes will be assessed by appropriate chromatographic, mass spectrometric or radiometric techniques. The phenotypic outcome of knocking-out these genes will be tested by microscopy, glycomics and virulence tests. In the longer term, identification and analysis of other genes involved in synthesis and transfer of galactofuranose and N-acetylneuraminic acid may prove to be interesting targets for further study. Tangible benefits for immunocompromised patients can be expected due to the development of novel effective therapeutics based on targetting of fungal-specific glycosylation pathways, thus resulting in a lack of side-effects for the patient.
- glycosylation
One single basic amino acid at the Ï-1 or Ï-2 site is a signal that retains glycosylphosphatidylinositol-anchored protein in the plasma membrane of Aspergillus fumigatus.
Autoren: Ouyang, H; Chen, X; Lü, Y; Wilson, IB; Tang, G; Wang, A; Jin, C Jahr: 2013
Journal articles
N-glycomic profiling of a glucosidase II mutant of Dictyostelium discoideum by ""off-line"" liquid chromatography and mass spectrometry.
Autoren: Hykollari, A; Dragosits, M; Rendić, D; Wilson, IB; Paschinger, K; Jahr: 2014
Journal articles
Kexin-like endoprotease KexB is required for N-glycan processing, morphogenesis and virulence in Aspergillus fumigatus
Autoren: Wang, JY; Zhou, H; Lu, H; Du, T; Luo, YM; Wilson, IBH; Jin, C Jahr: 2015
Journal articles
More Than Just Oligomannose: An N-glycomic Comparison of Penicillium Species.
Autoren: Hykollari, A; Eckmair, B; Voglmeir, J; Jin, C; Yan, S; Vanbeselaere, J; Razzazi-Fazeli, E; Wilson, IB; Paschinger, K; Jahr: 2016
Journal articles
Aspergillus fumigatus phosphoethanolamine transferase gene gpi7 is required for proper transportation of the cell wall GPI-anchored proteins and polarized growth
Autoren: Ouyang, HM; Du, T; Zhou, H; Wilson, IBH; Yang, JH; Latge, JP; Jin, C Jahr: 2019
Journal articles
Aspergillus fumigatus Mnn9 is responsible for mannan synthesis and required for covalent linkage of mannoprotein to the cell wall.
Autoren: Du, T; Ouyang, H; Voglmeir, J; Wilson, IBH; Jin, C; Jahr: 2019
Journal articles
Mitarbeiter*Innen
Iain B.H. Wilson
Ao.Univ.Prof. Dr.phil. Iain B.H. Wilson
iain.wilson@boku.ac.at
Tel: +43 1 47654-77216, 77217
BOKU Project Leader
01.07.2010 - 30.06.2014
Alba Hykollari
Dipl.-Ing. Dr. Alba Hykollari
alba.hykollari@boku.ac.at
Project Staff
01.07.2010 - 30.06.2014
Josef Voglmeir
Dipl.-Ing. Dr. Josef Voglmeir
josef.voglmeir@njau.edu.cn
Project Staff
01.07.2010 - 30.06.2014
BOKU Partner
Externe Partner
Chinese Academy of Sciences, Institute of Microbiology, Beijing
Cheng Jin
partner