Recombinant glycosidases as tools in research
Abstract
The importance of glycosylation events and resulting glycoconjugates in health and disease is well-known. The variability of glycoconjugates in nature, including also the more complex than previously believed glycomes of invertebrates, presents a considerable challenge as regards their structural analysis. Even the release of glycans from proteins or peptides requires careful consideration and various enzymatic or chemical approaches can be employed, which may result in a different set of glycans being isolated. Often the N-glycosidase from almonds is used to release N-glycans from glycopeptides. Furthermore, another glycosidase, Drosophila Fdl, removes specifically the N-acetylglucosamine attached to the alpha1,3-arm of N-glycans. In order to optimise the use of these enzymes, I propose to prepare recombinant forms of them and examine further their substrate requirements and optimal conditions. The end result is intended to be highly pure, well characterized and, if possible, immobilized forms of these enzymes, which should facilitate development of new, faster and robust methods for N-glycan analysis.
keywords N-glycan N-glycosidase (PNGase) N-acetylglucosaminidase (Fdl) Glycobiology Immobilization
Publikationen
SweetBac: A New Approach for the Production of Mammalianised Glycoproteins in Insect Cells
Autoren: Palmberger, D; Wilson, IBH; Berger, I; Grabherr, R; Rendic, D Jahr: 2012
Journal articles
Novel Recombinant Enyzmes for Glycobiotechnological Applications
Autoren: Dragosits M, Palmberger D, Kurz S, Razzazi-Fazeli E, Wilson Iain BH, Rendic D Jahr: 2012
Conference & Workshop proceedings, paper, abstract
SweetBac: a new approach for the production of mammalianised glycoproteins in insect cells
Autoren: Palmberger, D; Wilson, IBH; Berger, I; Grabherr, R; Rendic, D Jahr: 2012
Journal articles
Recombinant Aspergillus â-galactosidases as a robust glycomic and biotechnological tool.
Autoren: Dragosits, M; Pflügl, S; Kurz, S; Razzazi-Fazeli, E; Wilson, IB; Rendic, D Jahr: 2014
Journal articles
Recombinant glycosidases as tools in glycan analysis
Autoren: Dragosits, M; Kurz, S; Hykollari, A; Yan, S; Wilson, IBH; Rendic, D Jahr: 2013
Journal articles
N-glycomic profiling of a glucosidase II mutant of Dictyostelium discoideum by ""off-line"" liquid chromatography and mass spectrometry.
Autoren: Hykollari, A; Dragosits, M; Rendić, D; Wilson, IB; Paschinger, K; Jahr: 2014
Journal articles
Enzymatic properties and subtle differences in the substrate specificity of phylogenetically distinct invertebrate N-glycan processing hexosaminidases.
Autoren: Dragosits, M; Yan, S; Razzazi-Fazeli, E; Wilson, IB; Rendic, D; Jahr: 2015
Journal articles
Increasing Complexity of the N-Glycome During Caenorhabditis Development.
Autoren: Wilson, IBH; Yan, S; Jin, C; Dutkiewicz, Z; Rendić, D; Palmberger, D; Schnabel, R; Paschinger, K; Jahr: 2023
Journal articles
Project staff
Dubravko Rendic
Dipl.-Ing. Dr. Dubravko Rendic
dubravko.rendic@boku.ac.at
Tel: +43 1 47654-39071
Project Leader
01.06.2011 - 30.11.2015
Dieter Palmberger
Dipl.-Ing. Dr. Dieter Palmberger
dieter.palmberger@boku.ac.at
Tel: +43 1 47654-79859
Project Staff
01.06.2011 - 30.11.2015
BOKU partners
External partners
Institute of Nutrition
Prof. Dr. Ebrahim Razzazi
partner