Florian Rüker

Presentations

• Year: 2023 -  Combining Controlled Fab-arm exchange and mAb² for the production of asymmetric trispecific antibodies

  Autoren: Natale, V; Stadlmayr, G; Benedetti, F; Stadlbauer, K; Rüker, F; Wozniak-Knopp, G

  Event: PEGS Europe

• Year: 2023 -  Controlled Fab-arm exchange on mAb² pairs for the production of multispecific antibodies

  Autoren: Natale, V; Stadlmayr, G; Benedetti, F; Stadlbauer, K; Rüker, F; Wozniak-Knopp, G

  Event: PEGS Boston

• Year: 2022 -  Trispecific Antibodies produced from mAb² pairs by controlled Fab Arm exchange

  Autoren: Natale, V; Stadlmayr, G; Benedetti, F; Stadlbauer, K; Rüker, F; Wozniak-Knopp, G

  Event: Antibody Engineering & Therapeutics Europe

• Year: 2022 -  Circumventing immunization - The use of in vitro evolution and selection to make antibodies

  Autoren: Rüker, F;

  Event: ÖGMBT-Young Life Scientists Austria/FEBS Junior Sections - Talk Series 2022

• Year: 2022 -  CD81 large extracellular loop as a novel alternative scaffold: laminin binding CD81 enhances specific uptake of extracellular vesicles

  Autoren: Wozniak-Knopp, G; Vogt, S; Stadlmayr, G; Stadlbauer, K; Bobbili, MR; Kjems, J; Rüker, F; Grillari, J

  Event: 9th BBBB International Conference on Pharmaceutical Sciences 2022

• Year: 2022 -  Circumventing immunization - The use of in vitro evolution and selection to make monoclonal antibodies

  Autoren: Rüker, F;

  Event: Behördenseminar Bundesministerium für Bildung, Wissenschaft und Forschung Sektion V - Wissenschaftliche Forschung; Internationale Angelegenheiten Referat 3b - Gentechnik und Tierversuchswesen

• Year: 2021 -  Circumventing immunization by in vitro directed evolution and selection of human antibodies from large surface display libraries

  Autoren: Rüker, F;

  Event: Gesellschaft zur Förderung von alternativen Biomodellen (The RepRefRed Society)

• Year: 2021 -  Bispecific antibodies – new formats for cancer immunotherapy

  Autoren: Rüker, F;

  Event: BioSeparation Forum

• Year: 2021 -  An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: laminin binding CD81 enhances cellular uptake of extracellular vesicles

  Autoren: Vogt, S; Bobilli, MR, Stadlmayr, G; Stadlbauer, K; Rüker, F; Wozniak-Knopp G

  Event: Austrian Society for Extracellular Vesicles

• Year: 2019 -  Improving the stability of the large extracellular loop of human tetraspanin CD81

  Autoren: Stefan Vogt, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Rüker, Johannes Grillari, Gordana Wozniak-Knopp

  Event: International Society of Extracellular Vesicles - Annual Meeting 2019

• Year: 2019 -  Laminin-targeted extracellular vesicles reveal enhanced specific uptake in vitro

  Autoren: Stefan Vogt, Madhusudhan Reddy Bobbili, Florian Rüker, Johannes Grillari, Gordana Wozniak-Knopp

  Event: 1st Annual Symposium of the Danish Society for Extracellular Vesicles (DSEV)

• Year: 2017 -  Construction of a naive yeast-display Fab antibody library

  Autoren: Stadlmayr, G; Eibensteiner, K; Rüker, F; Wozniak-Knopp, G

  Event: Merck Biopharma Antibody Engineering ?Mini-Symposium

• Year: 2016 -  Antibody microarray for fast detection of methicillin-resistant Staphylococcus aureus

  Autoren: Krobath, E; Nöhammer, .; Rüker, F; Wozniak-Knopp, G; Hust, M; Wiesinger-Mayr, H

  Event: Austrian Biomarker Symposium 2016

• Year: 2016 -  Domain-Exchanged Antibody with potential for improved effector functions

  Autoren: Wozniak-Knopp, G; Perthold, JW; Stadlmayr, G; Stadlbauer, K; Rüker, F.

  Event: 8th ÖGMBT Annual Meeting 2016

• Year: 2016 -  Domain-Exchanged Antibody with potential for improved effector functions

  Autoren: Wozniak-Knopp, G; Perthold, JW; Stadlmayr, G; Stadlbauer, K; Rüker, F.

  Event: The 5th Thailand International Nanotechnology Conference

• Year: 2016 -  Domain-exchanged antibody with potentiated effector functions

  Autoren: Knopp, GW; Stadlmayr, G; Perthold, JW; Stadlbauer, K; Rueker, F

  Event: 8th ÖGMBT Annual Meeting 2016

• Year: 2016 -  Domain-Exchanged Antibody with Potentiated Effector Functions

  Autoren: Wozniak-Knopp, G; Perthold, JW; Stadlmayr, G; Stadlbauer, K; Rüker, F

  Event: 17th European Biotechnology Congress

• Year: 2015 -  Engineering bispecific antibodies using the SEED platform: Solving the light chain pairing problem

  Autoren: Dietrich, S; Alec W. Gross, AW; Wozniak-Knopp, G; Becker, S; Hock, B; Rüker, F;

  Event: 7th ÖGMBT Annual Meeting 2015

• Year: 2015 -  Novel bispecific SEED antibodies with specific light chain pairing

  Autoren: Dietrich, S; Gross, AW; Wozniak-Knopp, G; Becker, S; Hock B; Rüker, F;

  Event: The 1st Biologics & Biosimilars Congress

• Year: 2015 -  Engineering bispecific antibodies using the SEED platform: Solving the light chain pairing problem

  Autoren: Dietrich, S; Gross, AW; Wozniak-Knopp, G; Becker, S; Hock, B; Rüker, F;

  Event: 7th ÖGMBT Annual Meeting 2015

• Year: 2015 -  Creating a new Fab interface in bispecific antibodies using rational design and small mammalian cell-based libraries

  Autoren: Bönisch, M; Sevo, M; Becker, S; Hock, B; Rüker, F;

  Event: 8th Conference on Recombinant Protein Production RPP8 2015

• Year: 2015 -  Construction of a very large semi-synthetic human antibody library

  Autoren: Gerhard Stadlmayr, Gordana Wozniak-Knopp, Milica Ševo, Florian Rüker

  Event: Merck Serono Antibody Engineering ?Mini-Symposium

• Year: 2015 -  Engineering novel antibody biologics

  Autoren: Rüker, F;

  Event: Woche der Chemie 2015

• Year: 2015 -  Engineering Novel Antibody Biologics by Modular Antibody Technology

  Autoren: Rüker, F; Wozniak-Knopp, W;

  Event: Wilhelm Exner Lectures

• Year: 2015 -  Rational design of a new Fab interface for the generation of heterodimeric IgGs

  Autoren: Bönisch, M; Sevo, M; Becker, S; Hock, B; Rüker, F;

  Event: The Biologics Congress

• Year: 2015 -  scFv-Fc fusion-protein Microarrays for fast detection of methicillin-resistant Staphylococcus aureus

  Autoren: Krobath, E; Wozniak-Knopp, G; Hust, M; Rüker, F; Nöhammer, C; Wiesinger-Mayr, H

  Event: 7th Annual PEGS Europe Protein and Antibody engineering summit 2015

• Year: 2015 -  Modulares Antikörper Engineering

  Autoren: Rüker, F; Wozniak-Knopp, G;

  Event: Young Science-Initiative

• Year: 2015 -  Bispecific antibodies targeting VEGF and ErbB receptors

  Autoren: Wozniak-Knopp, G; Stadlmayr, G; Stadlbauer, K; Steger, F; Rüker, F

  Event: Affinity Proteomics Workshop

• Year: 2014 -  Directed evolution of antibody fragments for improved stability and resistance to aggregation by using yeast surface display

  Autoren: Lobner, E., Traxlmayr, M. W., Rüker, F., Obinger, C.

  Event: Human Antibodies and Hybridomas Conference 2014

• Year: 2014 -  Molecular antibody technology: novel formats and applications of antibodies with engineered antigen-binding constant domains

  Autoren: Rüker, F; Wozniak-Knopp, G;

  Event: Human Antibodies and Hybridomas Conference 2014

• Year: 2014 -  Stability assessment on a library scale – A rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.

  Autoren: Hasenhindl, C., Traxlmayr, M.W., Wozniak-Knopp, G ., Jones, P.C., Stadlmayr, G., Rüker, F., Obinger, C.

  Event: Human Antibodies and Hybridomas Conference 2014

• Year: 2014 -  Modular Antibody Technology

  Autoren: Wozniak-Knopp, G; Rüker F

  Event: Bioprocess International 2014

• Year: 2014 -  Advancing novel antibody biologics

  Autoren: Rüker, F;

  Event: 11th International Life Science Meeting 2014

• Year: 2014 -  Stability Assessment of Antigen binding Fc fragments (Fcabs) of Different Library Designs

  Autoren: Ravnikar, M; Wozniak-Knopp, G; Rüker, F

  Event: 6th ÖGMBT Annual Meeting 2014 - Life Sciences meet Entrepreneurship

• Year: 2014 -  Construction of pH-sensitive Her2 binding antibody fragment by directed evolution using yeast display

  Autoren: Lobner, E., Traxlmayr, M. W., Rueker, F., Obinger, C.

  Event: The 16th European Congress on Biotechnology

• Year: 2014 -  Tackling the light chain association problem in heterodimeric IgG

  Autoren: Bönisch, M; Dietrich, S; Sevo, M; Quendler, H; Becker, S; Günther, R; Hock, B; Rüker, F

  Event: Human Antibodies and Hybridomas Conference 2014

• Year: 2013 -  Strategies for the stabilization of Fc fragments

  Autoren: Ruker, F; Knopp, GW

  Event: European Biotechnology Congress 2013

• Year: 2013 -  Elemental labeled antibody fragments for LC-ICP-MS based immunoassays

  Autoren: Mairinger, T., Kretschy, D., Wozniak-Knopp, G. Rueker, F., Koellensberger, G. and Hann, S.

  Event: Metallomics 2013, 4th International symposium on metallomics

• Year: 2013 -  Engineering the Stability of Antibody Constant Domains

  Autoren: Wozniak-Knopp, G; Rüker, F;

  Event: Affinity 2013

• Year: 2013 -  Fcabs – antigen binding Fc regions

  Autoren: Rüker, F; Wozniak-Knopp, G;

  Event: 6th Alpbach Workshop on Affinity Proteomics

• Year: 2013 -  Engineering and Ensuring Stability of Antibodies and Antibody Fragments

  Autoren: Rüker, F; Wozniak-Knopp, G;

  Event: Optimize 2013 - Antibody Engineering & Discovery

• Year: 2013 -  Engineering and Ensuring Stability of Antibodies and Antibody Fragments

  Autoren: Rüker, F;

  Event: Optimize 2013 - Antibody Engineering & Discovery

• Year: 2013 -  Fcabs - Antigen Binding Fc Regions

  Autoren: Rüker, F;

  Event: 6th Alpbach Workshop on Affinity Proteomics

• Year: 2013 -  World-class biotech research base in Austria

  Autoren: Rüker, F;

  Event: BIO Europe 2013 - High-Level Programme for International Delegations

• Year: 2013 -  Strategies for the Stabilisation of Fc fragments

  Autoren: Rüker, F; Wozniak-Knopp, G;

  Event: European Biotechnology Congress 2013

• Year: 2012 -  Modular antibodies: Introducing antigen-binding sites in the Fc region of IgG

  Autoren: Ruker, F

  Event: Eurobiotech Agriculture Symposium 2012

• Year: 2012 -  Strategies for the stabilization of Fc fragments

  Autoren: Gordana Wozniak-Knopp, Johannes Stadlmann, Florian Rüker

  Event: FA0804 COST Action Molecular farming 2012 -Plants as a production platform for high value proteins

• Year: 2012 -  Stabilization of the Fc fragment of human IgG1 by engineered interdomain disulfide bonds

  Autoren: Wozniak-Knopp, G; Stadlmann, J; Rüker, F;

  Event: 9th International Conference on Protein Stability 2012 - From molecular level to market applications

• Year: 2012 -  Stability engineering of IgG1-Fc and construction of a “protein stability landscape” by high throughput sequencing

  Autoren: Traxlmayr, M.W.Faissner, M., Hasenhindl, C., Hackl, M., Stadlmayr, G., Rüker, F. and Obinger, C.

  Event: 9th International Conference on Protein Stability 2012 - From molecular level to market applications

• Year: 2012 -  Systematic analysis of the C-terminal loops of IgG1-Fc for their usability in Modular Antibody Engineering

  Autoren: Hasenhindl, C., Wozniak-Knopp1, G., Traxlmayr, M.W., Hackl, M., Stadlmayr, G., Rüker F. and Obinger C.

  Event: 9th International Conference on Protein Stability 2012 - From molecular level to market applications

• Year: 2012 -  Pre-clinical developments for bispecific mAb2s with optimal target selection

  Autoren: Rüker, F;

  Event: Protein and Antibody Engineering Summit PEGS Europe 2012

• Year: 2012 -  Measures to Enhance Half-Life and Stability (Preconference Short Course)

  Autoren: Rüker, F; Traxlmayr, M;

  Event: Protein and Antibody Engineering Summit PEGS Europe 2012

• Year: 2012 -  Stability engineering of human IgG1-Fc to resist boiling

  Autoren: Stadlmayr, G., Schaffner, I., Traxlmayr, M., Wozniak-Knopp, G., Hasenhindl, C., Rüker, F. and Obinger, C.

  Event: 9th International Conference on Protein Stability 2012 - From molecular level to market applications

• Year: 2012 -  F-Star Cambridge - Spinoff company from BOKU

  Autoren: Rüker, F;

  Event: ICA RECTORS & DEANS FORUM 2012 - Engagement of life science universities in the knowledge society and innovation networks

• Year: 2011 -  Stability engineering of the Fc fragment of human IgG1 by targeted mutagenesis

  Autoren: Stadlmayr, G., Wozniak-Knopp, G., Hasenhindl, C., Rüker, F., Obinger, C.

  Event: 6th Conference on Recombinant Protein Production RPP6 2011

• Year: 2011 -  Stability-engineering of human IgG1-Fc by using yeast display

  Autoren: Traxlmayr, M. W., Faissner, M., Stadlmayr, G., Rüker, F., Obinger, C.

  Event: PEGS - Protein and Antibody Engineering Summit 2011

• Year: 2011 -  Stability engineering of human IgG1-Fc and application in modular antibody engineering

  Autoren: Hasenhindl,C., Traxlmayr, M. W., Faissner, M., Stadlmayr, G., Rüker, F., Obinger, C.

  Event: 3rd PEGS Europe Protein & Antibody Engineering Summit 2011

• Year: 2010 -  Stabilization of the Fc fragment of human IgG1 by engineered intra-domain disulfide bonds

  Autoren: Gordana Wozniak-Knopp, Gerhard Stadlmayr, Florian Rüker

  Event: 24th Anual Symposium of The Protein Society 2010

• Year: 2010 -  Engineering antigen binding sites into the Fc region of IgG: biochemical, biophysical and immunological characterization

  Autoren: Florian Rüker, Gordana Wozniak-Knopp

  Event: 24th Anual Symposium of The Protein Society 2010

• Year: 2010 -  Stability engineering of the Fc fragment of human IgG1 by targeted mutagenesis

  Autoren: Stadlmayr, G., Wozniak-Knopp, G., Hasenhindl, C., Rüker, F., Obinger, C.

  Event: 5th Central European Conference on Chemistry meets biology

• Year: 2010 -  Engineering antigen binding sites into the Fc region of IgG

  Autoren: Florian Rüker, Gordana Wozniak-Knopp

  Event: Advancing Protein Therapeutics

• Year: 2010 -  Stabilization of the Fc fragment of human IgG1 by engineered intra-domain disulfide bonds

  Autoren: Gordana Wozniak-Knopp, Gerhard Stadlmayr, and Florian Rüker

  Event: 2nd ÖGMBT Annual Meeting 2010

• Year: 2010 -  Introducing antigen-binding sites into structural loops of immunoglobulin constant domains

  Autoren: Gordana Wozniak-Knopp, Stefan Bartl, Anton Bauer, Marwa Mostageer, Max Woisetschläger, Bernhard Antes, Karl Ettl, Manuela Kainer, Gertrude Weberhofer, Susanne Wiederkum, Gottfried Himmler, Geert Mudde, and Florian Rüker

  Event: 2nd ÖGMBT Annual Meeting 2010

• Year: 2009 -  Fcab and mAb2: biologically active and antigen-specific immunoglobulin Fc molecules derived from yeast display libraries with antigen binding sites in the IgG1-CH3 domain

  Autoren: Florian Rüker

  Event: Protein Engineerung Summit 2009

• Year: 2009 -  Immunoglobulin Fc as a Scaffold for Antigen Binding (Fcabs):Long Half Life and Effector Functions

  Autoren: Gordana Wozniak-Knopp

  Event: Protein Engineering Summit Europe 2009

• Year: 2009 -  Fcab and mAb2 Biologically active and antigen-specific immunoglobulin Fc molecules derived from yeast display libraries with antigen binding sites in the IgG1-CH3 domain

  Autoren: Florian Rüker

  Event: 3rd International Symposium on Antibody Engineering and Antibody-based Therapeutics (AEAT 2009)

• Year: 2009 -  Engineered human IgG1-Fc binding to integrin

  Autoren: Traxlmayr, M., Wozniak-Knopp, G., Rüker, F., Obinger, C.

  Event: 20th IBC's Annual International Conference on Antibody Engineering 2009

• Year: 2008 -  Introducing Modular Antibody Technology

  Autoren: Florian Rüker

  Event: Antibody Engineering Asia 2008

• Year: 2007 -  Introducing Modular Antibody Technology

  Autoren: Florian Rüker

  Event: IBC’s 18th Annual International Antibody Engineering Conference 2007

• Year: 2005 -  Protein Engineering am IAM

  Autoren: Florian Rüker

  Event: 60 Year Anniversary, Institute of Applied Microbiology IAM 2005

• Year: 2005 -  Cloning, Expression, Purification and Characterization of human Alpha-Fetoprotein

  Autoren: Michael Dekner, Gordana Wozniak-Knopp and Florian Rüker

  Event: 60 Year Anniversary, Institute of Applied Microbiology IAM 2005

• Year: 2005 -  Selection and characterization of phage-display scFvs directed against Ep-CAM

  Autoren: Gordana Wozniak-Knopp and Florian Rüker

  Event: 60 Year Anniversary, Institute of Applied Microbiology IAM 2005

• Year: 2003 -  Engineering catalase-peroxidases: The structural basis of the activity of catalase-peroxidases.

  Autoren: Petutschnig, G., Jakopitsch, C., Auer, M., Rüker, F., Furtmüller, P. G., and Obinger, C.

  Event: Eigth International Summer School on Biophysics

• Year: 2003 -  Do we really understand the catalatic reactivity?

  Autoren: Jakopitsch, C; Petutschnig, G; Zehner, F; Ruker, F; Furtmuller, PG; Obinger, C

  Event: Plant Stress, Reactive Oxygen and Antioxidants

• Year: 2003 -  Converting Myeloperoxidase to Lactoperoxidase

  Autoren: Zederbauer, M., Ciaccio, C., Moguilevsky, N., Jantschko, W., Furtmüller, P. G., and Obinger, C.

  Event: Eigth International Summer School on Biophysics

• Year: 2002 -  Cloning and hererologous expression of Sclerotium rolfsii CDH gene in Pichia pastoris with its own signal peptide.

  Autoren: Zamocky, R. Ludwig, F. Rüker, and D. Haltrich

  Event: 6th European Conference on Fungal Genetics 2002

• Probing active site residues of catalase-peroxidases.

  Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek , G.A., Obinger, C.

• Catalase versus peroxidase activity. Towards understanding the bifunctional activity of catalase-peroxidases.

  Autoren: Regelsberger, G., Jakopitsch, C., Rüker, F., Loewen, P., Peschek, G. A., Obinger, C.

• Characterization of the superoxide dismutases in the filamentous cyanobacterium Anabaena variabilis.

  Autoren: Regelsberger, G., Laaha, U., Dagmar, D., Rüker, F., Peschek, G.A., Obinger, C.

• Cloning, overexpression and characterization of a cyanobacterial monofunctional catalase.

  Autoren: Schwaiger, H., Regelsberger, G., Rueker, F., Peschek, G.A., Zámocky, M., Obinger, C.

• Manipulating the distal asparagine-histidine couple in catalase-peroxidase.

  Autoren: Jakopitsch, C., Regelsberger, G., Rücker, F., Peschek, G.A., Obinger, C.

• Iron and manganese containing cyanobacterial superoxide dismutases.

  Autoren: Regelsberger, G., Laaha, U., Dietmann, D., Rüker, F., Peschek, G.A., Obinger, C.

• Hydrogen peroxide removal in Anabaena variabilis: Cloning, overexpression and characterization of a thioredoxin peroxidase.

  Autoren: Plasser, L., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.

• Catalase versus peroxidase activity. Towards understanding the bifunctional activity of catalase-peroxidases.

  Autoren: Regelsberger, G., Jakopitsch, C., Rüker, F., Peschek, G.A., Switala, J., Loewen, P., Obinger, C.

• The cyanobacterial aa3-type cytochrome c oxidase: Biochemical characterization and expression of the Synechocystis sp. PCC6803 cta genes in Rhodobacter sphaeroides.

  Autoren: Paumann, M., Hiser, C., Rüker, F., Obinger, C., Ferguson-Miller, S., Peschek, G.A.

• Binding of peroxides and inhibitors to the active site of catalase-peroxidases.

  Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.

• The catalatic acitivity of catalase-peroxidases.

  Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.

• Molecular engineering of catalase-peroxidases.

  Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.

• Binding of peroxides and inhibitors to the active site of catalase-peroxidases.

  Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.

• The molecular and functional peculiarities of class I peroxidases.

  Autoren: Obinger, C., Regelsberger, G., Jakopitsch, C., Peschek, G.A., Rüker, F.

• Molecular and kinetic characterization of cyanobacterial iron- and manganese-containing superoxide dismutases.

  Autoren: Regelsberger, G., Laaha, U., Dietmann, D., Rüker, F., Peschek, G.A., Obinger, C.

• Hydroperoxidases in oxygenic phototrophic prokaryotes- comparison to plant chloroplasts

  Autoren: Regelsberger, G., Jakopitsch, C., Rücker, F., Peschek, G.A., Obinger, C.

• Cloning, overexpression and characterization of a cyanobacterial thioredoxin peroxidase.

  Autoren: Plasser, L., Regelsberger, G., Rueker, F., Peschek, G. A., Obinger, C.