Stefan Hofbauer
Ass.Prof. Priv.-Doz. Dipl.-Ing. Stefan Hofbauer Ph.D.
Institute of Biochemistry (DCH/BC)
Location Muthgasse 18, 1190 Wien
Email stefan.hofbauer@boku.ac.at
Tel: +43 1 47654-77258
ORCID: 0000-0003-3375-7715
I-3889-2014: ResearcherId
15759623700: AuthorId
https://short.boku.ac.at/HemeBiosynthesis: persönliche Webseite
Research Focus
Heme biosynthesis is essential for almost all living organisms. Many pathogens utilize the "coproporhyrin-dependent" heme biosynthesis pathway, which was discovered in 2015.
We study the biochemistry of enzymes involved in prokaryotic heme biosynthesis in order to understand structure-function relationships with the ultimate goal to unravel each enzymes' reaction mechanism and their protein-protein interactions.
This basic research questions, which we address, pave the way to find novel antibiotic substances, which are highly needed given the emergence of many superbugs, exhibiting multiple resistances towards common antibiotic treatments.
Career
- 2021 Habilitation "Molecular Enzymology" (venia docendi)
- 2016 Post-Doc at the department of chemistry in the protein biochemistry group at the University for Natural Resources and Life Sciences, Vienna
- 2014 - 2016 Post Doc at Department for Structural and Computational Biology, University of Vienna, Max F. Perutz Laboratories
- 2013 Research stay at the Department of Chemistry at the University of Florence, Italy
- 2011 Research stay at the Department of Chemistry at the University of Modena and Reggio Emilia, Italy
- 2011 - 2014 PhD studies (doctoral program BioToP) at the department of chemistry at the University for Natural Resources and Life Sciences, Vienna
- 2010 Research stay (master thesis) at the Instituto de Tecnologia Quimica e Biologica Antonio Xavier, New University of Lisbon, Portugal
- 2008 Research stay at the Department of for Applied Biology and Chemical Technology at the Hong Kong Polytechnic University
- 2007 Research stay at the Max-Planck-Institute for Biochemistry, Martinsried Germany
- 2004 - 2011 studies in Food Science and Biotechnology (Bachelor degree 2008), and Master studies in Biotechnology at BOKU - University of Natural Resources and Life Sciences
Projects
0 Projects found.
Publications
Knowledge Transfer to Society
Media
Lectures
Year: 2024 - Understanding molecular enzymology of porphyrin-binding dimeric α + β barrel proteins
Autoren: Hofbauer, S
Event: Minisymposium on Peroxygenase Discovery, Engineering, Production and Application 2024
Year: 2023 - Molecular mechanisms of prokaryotic heme biosynthesis enzymes
Autoren: Hofbauer, S; Patil, G; Gabler, T; Falb, N; Furtmüller PG
Event: UMHM - Unraveling Mysteries of Heme Metabolism
Year: 2022 - Biochemistry of Enzymes Involved in the Coproporphyrin-Dependent Heme Biosynthesis Pathway of Gram-Positive Bacteria
Autoren: Hofbauer, S; Gabler, T; Patil, G; Michlits, H; Pfanzagl, V; Obinger, C; Furtmüller, PG
Event: Gordon Research Conference - Chemistry and Biology of Tetrapyrroles
Year: 2022 - Spectroscopic characterization of the coproporphyrin III coproporphyrin ferrochelatase complex of Listeria monocytogenes
Autoren: Dali, A; Sebastiani, S; Gabler, T; Obinger, C; Furtmüller, PG; Becucci, M; Hofbauer, S; Smulevich, G
Event: OxiZymes 2022
Year: 2022 - Reactivity of peroxidases and chlorite dismutases with chlorite and hypochlorite: a spectroscopic investigation of short-lived intermediates trapped by fast freeze-quenching
Autoren: Serra, I; Schmidt, D; Hofbauer, S; Pfanzagl, V; García-Rubio, I; Furtmüller, PG; Obinger C; and Van Doorslaer, V;
Event: The 55th Annual International Meeting of the ESR Spectroscopy Group of the Royal Society of Chemistry 2022
Year: 2022 - Molecular mechanism of the last step of the heme b biosynthesis in Gram-positive bacteria: the rotation matters
Autoren: Sebastiani, F; Michlits, H; Dali, A; Becucci, M; Hofbauer, S; Smulevich, G
Event: 12th International Conference on Porphyrins and Phtalocyanines ICPP-12 2022
Year: 2022 - X-ray induced reduction of heme metal centers is protein-independent
Autoren: Pfanzagl, V; Beale, JH; Michlits, H; Schmidt, D; Gabler, T; Obinger, C; Djinovic-Carugo, K; Hofbauer, S;
Event: 33rd European Crystallographic Meeting
Year: 2022 - Structural and mechanistic insights in dimeric chlorite dismutase - Impact of pH and the dynamics of the catalytic arginine
Autoren: Schmidt, D; Serra, I; Hofbauer, S; Pfanzagl, V; Van Doorslaer, S; Obinger, C; Furtmüller, P.G.
Event: 16th European Biological Inorganic Chemistry 2022
Year: 2022 - Importance of H-bonds on substrate specificity and complex stability of firmicute coproporphyrin ferrochelatase
Autoren: Gabler, T; Dali, A; Sebastiani, S; Helm, J; Obinger, C; Furtmüller, PG; Smulevich, G; Hofbauer, S
Event: OxiZymes 2022
Year: 2021 - Coproporphyrin ferrochelatase – study of relevant residues for substrate and product binding
Autoren: Gabler, T; Pfanzagl, V; Obinger, C; Furtmüller PG; Hofbauer, S
Event: International Conference on Porphyrins and Phthalocyanines - ICPP11
Year: 2019 - Interaction of hydrogen sulfide with myeloperoxidase in presence of hydrogen peroxide or oxygen
Autoren: Furtmüller, PG; Palinkas, Z; Garai, D; Rios-Gonzalez, BB; Lopez-Garriga, J; Hofbauer, S; Obinger, C; Nagy, P
Event: 11th Human Peroxidase Meeting
Year: 2019 - Resonance Raman to understand the heme cavity impact on the structure and function of coproheme decarboxylase (ChdC) from Listeria monocytogenes
Autoren: Milazzo, L; Hofbauer, S; Gabler, T; Furtmüller, PG; Obinger, C; Smulevich, G
Event: 10th International Conference on Advanced Vibrational Spectroscopy
Year: 2019 - Posttranslational Modification of Heme in Peroxidases - Impact on Structure and Function
Autoren: Furtmüller, PG; Nicolussi, A; Sevcnikar, B; Paumann-Page, M; Auer, M; Zamocky, M; Pfanzagl, V; Hofbauer, S; Obinger, C
Event: 19th International Conference on Biological Inorganic Chemistry 2019
Year: 2019 - YfeX - A new player involved in the redox gated potassium efflux pump KefC
Autoren: Pfanzagl, V; Schwaiger, L; Michlits, H; Hofbauer, S; Obinger, C
Event: Gordon Research Conference: Mechanisms of Membrane Transport
Year: 2019 - Role of H98Y variant of human myoglobin in Myoglobinopathy
Autoren: Hofbauer, S; Bernini, C; Pignataro, M; Obinger, C; Battistuzzi, G; Furtmüller, PG
Event: 11th Human Peroxidase Meeting
Year: 2019 - An actinobacterial representative sheds a new light on the reaction mechanism of coproheme decarboxylases
Autoren: Michlits, H; Pfanzagl, V; Furtmüller, PG; Djinovic-Carugo, K; Obinger, C; Hofbauer, S
Event: 19th International Conference on Biological Inorganic Chemistry 2019
Year: 2019 - Molecular mechanism of coproheme to heme b conversion
Autoren: Hofbauer, S; Milazzo, L; Gabler, T; Pühringer, D; Pfanzagl, V; Djinovic-Carugo, K; Furtmüller, PG; Obinger, C; Smulevich, G
Event: 19th International Conference on Biological Inorganic Chemistry 2019
Year: 2019 - Molecular Mechanism of Enzymatic Chlorite Degradation
Autoren: Obinger, C; Schaffner, I; Schmidt, D; Smulevich, G; Coates, L; Battistuzzi, G; Furtmüller, PG; Hofbauer, S
Event: 19th International Conference on Biological Inorganic Chemistry 2019
Year: 2019 - X-ray induced redcution kinetics of heme metal centers is protein-independent - implications for structural studies of redox sensitive proteins
Autoren: Pfanzagl, V; Beale, J; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, K; Obinger, C; Hofbauer, S
Event: 19th International Conference on Biological Inorganic Chemistry 2019
Year: 2019 - X-ray induced reduction of heme metal centers is protein-independent – implications for structural studies of redox sensitive proteins
Autoren: Pfanzagl, V; Beale, JH; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, C; Obinger, C; Hofbauer, S
Event: Hot Topics in Crystallography 4
Year: 2019 - X-ray induced redcution kinetics of heme metal centers is protein-independent
Autoren: Pfanzagl, V; Beale, J; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, K; Obinger, C; Hofbauer, S
Event: 11th Human Peroxidase Meeting
Year: 2019 - Manipulation of heme environment in the active site of chlorite dismutases and its impact on the enzymatic activity
Autoren: Schmidt, D; Schaffner, I; Hofbauer, S; Furtmüller PG; Obinger, C
Event: 19th International Conference on Biological Inorganic Chemistry 2019
Year: 2019 - Posttranslational modification of heme - Impact on generation of hypohalous acids
Autoren: Obinger, C; Nicolussi, A; Auer, M; Sevcnikar, B; Paumann-Page, M; Hofbauer, S; Furtmüller, PG;
Event: 11th Human Peroxidase Meeting
Year: 2018 - Coproheme decarboxylase from Listeria monocytogenes: insight into the active site structure
Autoren: Milazzo, L; Hofbauer, S; Howes, BD; Gabler, T; Furtmüller, PG; Obinger, C; Smulevich, G.
Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
Year: 2018 - Characterization and comparison of the distal heme environment of dimeric and pentameric chlorite dismutases
Autoren: Schmidt, D., Schaffner, I., Hofbauer, S., Mlynek, G., Djinović-Carugo, K., Furtmüller, P. G., Obinger, C.
Event: OxiZymes 2018
Year: 2018 - Mechanistic Studies on Coproheme Decarboxylase
Autoren: Hofbauer, S; Milazzo, L; Mlynek, G; Gabler, T; Howes, BD; Pfanzagl, V; Djinović-Carugo, K; Furtmüller, PG; Smulevich, G; Obinger, C
Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
Year: 2018 - Crystal and Electronic Structure of Compound I in Dye-decolorizing Peroxidases
Autoren: Pfanzagl, V; Hofbauer, S; Beale, J; Mlynek, G; Michlits, H; Nys, K; Van Doorslaer, S; Djinovic-Carugo, K; Furtmüller, PG; Obinger, C
Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
Year: 2018 - Kinetic and spectroscopic characterization of EfeB
Autoren: Michlits, H., Pfanzagl, V., Schwaiger, L., Furtmüller, P. G., Hofbauer, S., Obinger, C.
Event: OxiZymes 2018
Year: 2018 - On Compound I formation in B-type dye decolorizing peroxidases - lessons from structural and kinetic studies.
Autoren: Pfanzagl, V., Nys, K., Mlynek, G., Djinović-Carugo, K., Van Doorslaer, S., Furtmüller, P. G., Hofbauer, S., Obinger, C.
Event: OxiZymes 2018
Year: 2018 - Molecular mechanism of enzymatic chlorite degradation
Autoren: Obinger, C; Schaffner, I; Smulevich, G; Coates, L; Battistuzzi, G; Furtmüller, P.G; Hofbauer, S;
Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
Year: 2018 - Coproheme decarboxylases – phylogenetic prediction versus biochemical analysis.
Autoren: Pfanzagl, V., Holcik, L., Maresch, D., Michlits, H., Furtmüller, P. G., Obinger, C., Hofbauer, S.
Event: OxiZymes 2018
Year: 2018 - Posttranslational Modification of Heme in Peroxidases – Impact on Structure and Catalysis
Autoren: Furtmüller, PG; Nicolussi, A; Auer, M; Paumann-Page, M; Sevcnikar, B; Pfanzagl, V; Zámocký, M; Hofbauer, S; Obinger, C;
Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
Year: 2018 - Coproheme Listeria monocytogenes HemQ and selected mutants to highlight the structure function
Autoren: Milazzo, L; Hofbauer, S; Howes, BD; Obinger, C; Smulevich, G
Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)
Year: 2017 - How heme to protein bonds modulate reactivity - Lessons from a bacterial peroxidase with homology to lactoperoxidase
Autoren: Furtmüller, P.G; Nicolussi, A; Auer, M; Hofbauer, S; Bellei, M; Battistuzzi, G; and Obinger, C
Event: 10th International Human Peroxidase Meeting - Breckenridge 2017
Year: 2017 - Roles of the distal Asp-Arg pair in compound I formation of K. pneumoniae dye-decolorizing peroxidase
Autoren: Pfanzagl, V; Hofbauer, S; Nys, K; Djinović-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmüller, P.G; and Obinger, C;
Event: International Conference on Bioinorganic Chemistry ICBIC-18 2017
Year: 2017 - Pre-steady-state kinetics reveal the substrate specifity and mechanism of halide oxidation of turncated human peroxidasin
Autoren: Paumann-Page, M; Katz, R.-S; Bellei, M; Schwartz, I; Edenhofer, E; Sevcnikar, B; Soudi, M; Hofbauer, S; Battistuzzi, G; Furtmüller, P.G; and Obinger, C;
Event: 10th International Human Peroxidase Meeting - Breckenridge 2017
Year: 2017 - Roles of the distal Asp-Arg pair in Compound I formation of K. pneumoniae dye-decolorizing peroxidase
Autoren: Pfanzagl, V; Hofbauer, S; Nys, K; Djinovic-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmuller, PG; Obinger, C
Event: International Conference on Bioinorganic Chemistry ICBIC-18 2017
Year: 2016 - High-resultion X-ray and neutron diffration structures and biophysical studies on a novel dimeric chlorite dismutase
Autoren: Schaffner, I., Mlynek, G., Hofbauer, S., Smulevich, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Coates, L., Obinger, C.
Event: 79th Harden Conference on Oxygen Evolution and Reduction - Common Principles
Year: 2016 - Chlorite dismutase: Combining X-ray/neutron crystallography to investigate structure-function relationships
Autoren: Schaffner, I; Mlynek, G; Coates, L; Hofbauer, S; Djinović-Carugo, K; Furtmüller, P.G; Obinger, C
Event: OxiZymes 2016
Year: 2016 - Chlorite dismutase: distal heme environment and its role in irreversible enzyme inactivation
Autoren: Schaffner, I., Hofbauer, S., Mlynek, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Obinger, C.
Event: 13th European Biological Inorganic Chemistry Conference EuroBIC 2016
Year: 2016 - Dye decolorizing peroxidases - A new heme-peroxidase family with ancient roots
Autoren: Pfanzagl, V; Schaffner, I; Hofbauer, S; Furtmuller, P; Obinger, C
Event: European Congress on Biotechnology 2016
Year: 2016 - Dioxygen evolution by chlorite dismutase depends on co-ordination of transiently produced hypochlorite to an oxoiron(IV) intermediate
Autoren: Hofbauer, S., Grünwald-Gruber, C., Schaffner, I., Sündermann, A., Jakopitsch, C., Oostenbrink, C., Djinovic-Carugo, K., Furtmüller, P.G., Obinger, C.
Event: 79th Harden Conference on Oxygen Evolution and Reduction
Year: 2016 - Dye decolorizing peroxidases – a new heme-peroxidase family with ancient roots
Autoren: Pfanzagl, V; Schaffner, I; Hofbauer, S; Furtmüller, P.G; Obinger, C
Event: 17th European Congress on Biotechnology ECB17 2016
Year: 2016 - Coproheme and HemQ
Autoren: Hofbauer, S., Scheiblbrandner, S., Schaffner, I., Djinović-Carugo, K., Battistuzzi, G., Furtmüller, P. G., Obinger, C.
Event: 13th European Biological Inorganic Chemistry Conference EuroBIC 2016
Year: 2014 - Mechanistic studies on a new functional dimeric chlorite dismutase
Autoren: Schaffner, I; Flego, N; Mlynek, G; Bellei, M; Hofbauer, S; Battistuzzi, G; Djinovic-Carugo, K; Smulevich, G; Furtmuller, PG; Obinger, C
Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014
Year: 2014 - Mechanism of reaction of chlorite with mammalian peroxidases
Autoren: Jakopitsch, C; Pirker, KF; Flemmig, J; Hofbauer, S; Schlorke, D; Furtmuller, PG; Arnhold, J; Obinger, C
Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014
Year: 2014 - Chlorite inactivates mammalian peroxidases
Autoren: Jakopitsch, C., Pirker, K.F., Flemmig, J. Hofbauer, S., Furtmüller, P.G., Schlorke, D., Arnhold, J., Obinger, C.
Event: OxiZymes 2014
Year: 2014 - Biochemical and biophysical characterization of a new functional chlorite dismutase
Autoren: Schaffner, I., Hofbauer, S., Pirker, K.F., Furtmüller, P.G., Obinger, C.
Event: OxiZymes 2014
Year: 2014 - Understanding the inhibition mechanism of chlorite dismutase from the nitrite-oxidizing bacterium Candidatus Nitrospira defluvii
Autoren: Hofbauer, S; Gruber, C; Schaffner, I; Pirker, KF; Jakopitsch, C; Sundermann, A; Oostenbrink, C; Furtmuller, PG; Obinger, C
Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014
Year: 2014 - Chlorite to chloride and O2 conversion: new lessons from structural and mechanistic investigations of chlorite dismutase
Autoren: Obinger, C., Hofbauer, S., Schaffner, I., Pirker, K. F., Mlynek, G.,Djinovic-Carugo, K., Battistuzzi, G., Furtmüller, P. G
Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014
Year: 2014 - Redox properties of the Fe3+/Fe2+ couple in wild-type and mutated heme chlorite dismutases
Autoren: Hofbauer, S., Bellei, M., Schaffner, I., Furtmüller, P.G., Obinger, C., Battistuzzi, C.
Event: OxiZymes 2014
Year: 2014 - Characterization of the Major Peptidoglycan Hydrolase of Lactobacillus buchneri
Autoren: Anzengruber, J; Courtin, P; Pabst, M; Neumann, L; Claes IJJ; Debreczeny, M; Hofbauer, S; Obinger, C; Altmann, F; Chapot-Chartier, M-P; Vanderleyden, J; Messner, P; Schäffer, C
Event: FASEB Science Research Conference on Microbial Glycobiology
Year: 2014 - Chlorite dismutases and dye-decolorizing peroxidases – similarities and differences within a structural superfamily of heme proteins
Autoren: Schaffner, I., Hofbauer, S., Furtmueller, P. G., Obinger, C.
Event: 16th European Congress on Biotechnology 2014
Year: 2014 - Mechanism of inhibition of chlorite dismutase
Autoren: Hofbauer, S., Schaffner, I., Gruber, C., Pirker, K.F., Jakopitsch, C., Sündermann, A., Oostenbrink, C., Furtmüller, P.G., Obinger, C.
Event: OxiZymes 2014
Year: 2013 - New highly stable chlorinating bacterial peroxidase with autocatalytically formed covalent heme to protein bonds
Autoren: Furtmuller, PG; Auer, M; Gruber, C; Pirker, K; Krioss, D; Hofbauer, S; Soudi, M; Obinger, C; Zamocky, M; Bellei, M; Battistuzzi, G
Event: International Conference on Bioinorganic Chemistry ICBIC-16 2013
Year: 2013 - Understanding Chlorite Dismutase from Candidatus Nitrospira defluvii
Autoren: Hofbauer, S; Gruber, C; Pirker, KF; Schaffner, I; Furtmuller, PG; Obinger, C; Hagmuller, A; Gysel, K; Mlynek, G; Kostan, J; Djinovic-Carugo, K; Bellei, M; Battistuzzi, G; Daims, H
Event: International Conference on Bioinorganic Chemistry ICBIC-16 2013
Year: 2013 - Spectroelectrochemical characterization of heme enzymes
Autoren: Bellei, M., Hofbauer, S., Furtmüller, P. G., Obinger, C., Battistuzzi, G.
Event: International workshop on protein electron transfer: from fundamentals to applications for health
Year: 2013 - Understanding highly stable chlorite dismutase form nitrate-oxidizing bacterium Candidatus “Nitrospira defluvii”
Autoren: Hofbauer, S., Gruber, C., Pirker, K. F., Schaffner, I., Hagmüller, A., Gysel, K., Mlynek, G., Kostan, J., Bellei, M., Daims, H., Djinovic-Carugo, K. Battistuzzi, G., Furtmüller, P.G. and Obinger, C
Event: International Conference on Bioinorganic Chemistry ICBIC-16 2013
Year: 2013 - Spectroscopic Characterization of wild-type Chlorite Dismutase from Candidatus “Nitrospira defluvii” and selected variants
Autoren: Hofbauer, S., Flego, N., Schaffner, I., Pirker, K. F., Furtmüller, P. G., Howes, B. D., Obinger, C., Smulevich, G.
Event: 15th European Conference on the Spectroscopy of Biological Molecules
Year: 2012 - Redox theromdynamics of diverse chlorite dismutases
Autoren: Hofbauer, S. Bellei, M. Sündermann, A. Kostan, J. Daims, H. Djinović-Carugo, K. Oostenbrink, C. Furtmüller, P.G. Battistuzzi, G. & Obinger, C.
Event: OxiZymes 2012
Year: 2012 - Thermal and Conformational Stability of Pentameric Chlorite Dismutase from Candidatus Nitrospira defluvii
Autoren: Hofbauer, S., Gysel, K., Mlynek, G., Kostan, J., Daims, H., Furtmüller, P.G., Djinovics-Carugo, K. and Obinger,C.
Event: 9th International Conference on Protein Stability 2012 - From molecular level to market applications