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Stefan Hofbauer

Ass.Prof. Priv.-Doz. Dipl.-Ing. Stefan Hofbauer Ph.D.
Institute of Biochemistry (DCH/BC)
Location Muthgasse 18, 1190 Wien
Email stefan.hofbauer@boku.ac.at
Tel: +43 1 47654-77258

ORCID: 0000-0003-3375-7715
I-3889-2014: ResearcherId
15759623700: AuthorId
https://short.boku.ac.at/HemeBiosynthesis: persönliche Webseite

My Courses

Research Focus

Heme biosynthesis is essential for almost all living organisms. Many pathogens utilize the "coproporhyrin-dependent" heme biosynthesis pathway, which was discovered in 2015.

We study the biochemistry of enzymes involved in prokaryotic heme biosynthesis in order to understand structure-function relationships with the ultimate goal to unravel each enzymes' reaction mechanism and their protein-protein interactions.

This basic research questions, which we address, pave the way to find novel antibiotic substances, which are highly needed given the emergence of many superbugs, exhibiting multiple resistances towards common antibiotic treatments.

free keywords heme biosynthesis, molecular enzymology, structure function relationships,

 

Career

  • 2021 Habilitation "Molecular Enzymology" (venia docendi)
  • 2016 Post-Doc at the department of chemistry in the protein biochemistry group at the University for Natural Resources and Life Sciences, Vienna
  • 2014 - 2016 Post Doc at Department for Structural and Computational Biology, University of Vienna, Max F. Perutz Laboratories
  • 2013 Research stay at the Department of Chemistry at the University of Florence, Italy
  • 2011 Research stay at the Department of Chemistry at the University of Modena and Reggio Emilia, Italy
  • 2011 - 2014 PhD studies (doctoral program BioToP) at the department of chemistry at the University for Natural Resources and Life Sciences, Vienna
  • 2010 Research stay (master thesis) at the Instituto de Tecnologia Quimica e Biologica Antonio Xavier, New University of Lisbon, Portugal
  • 2008 Research stay at the Department of for Applied Biology and Chemical Technology at the Hong Kong Polytechnic University
  • 2007 Research stay at the Max-Planck-Institute for Biochemistry, Martinsried Germany
  • 2004 - 2011 studies in Food Science and Biotechnology (Bachelor degree 2008), and Master studies in Biotechnology at BOKU - University of Natural Resources and Life Sciences

Projects

0 Projects found.

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    Publications

    Knowledge Transfer to Society

    Media

    Lectures

    • Year: 2024 -  Understanding molecular enzymology of porphyrin-binding dimeric α + β barrel proteins

      Autoren: Hofbauer, S

      Event: Minisymposium on Peroxygenase Discovery, Engineering, Production and Application 2024

    • Year: 2023 -  Molecular mechanisms of prokaryotic heme biosynthesis enzymes

      Autoren: Hofbauer, S; Patil, G; Gabler, T; Falb, N; Furtmüller PG

      Event: UMHM - Unraveling Mysteries of Heme Metabolism

    • Year: 2023 -  Reactivity and protein-protein interaction of human peroxidasin 1

      Autoren: Furtmüller, P.G, Paumann-Page, M; Pfanzagl, V; Hofbauer, S; Obinger, C;

      Event: 12th International Human Peroxidase Meeting

    • Year: 2022 -  Biochemistry of Enzymes Involved in the Coproporphyrin-Dependent Heme Biosynthesis Pathway of Gram-Positive Bacteria

      Autoren: Hofbauer, S; Gabler, T; Patil, G; Michlits, H; Pfanzagl, V; Obinger, C; Furtmüller, PG

      Event: Gordon Research Conference - Chemistry and Biology of Tetrapyrroles

    • Year: 2022 -  Spectroscopic characterization of the coproporphyrin III coproporphyrin ferrochelatase complex of Listeria monocytogenes

      Autoren: Dali, A; Sebastiani, S; Gabler, T; Obinger, C; Furtmüller, PG; Becucci, M; Hofbauer, S; Smulevich, G

      Event: OxiZymes 2022

    • Year: 2022 -  Reactivity of peroxidases and chlorite dismutases with chlorite and hypochlorite: a spectroscopic investigation of short-lived intermediates trapped by fast freeze-quenching

      Autoren: Serra, I; Schmidt, D; Hofbauer, S; Pfanzagl, V; García-Rubio, I; Furtmüller, PG; Obinger C; and Van Doorslaer, V;

      Event: The 55th Annual International Meeting of the ESR Spectroscopy Group of the Royal Society of Chemistry 2022

    • Year: 2022 -  Molecular mechanism of the last step of the heme b biosynthesis in Gram-positive bacteria: the rotation matters

      Autoren: Sebastiani, F; Michlits, H; Dali, A; Becucci, M; Hofbauer, S; Smulevich, G

      Event: 12th International Conference on Porphyrins and Phtalocyanines ICPP-12 2022

    • Year: 2022 -  X-ray induced reduction of heme metal centers is protein-independent

      Autoren: Pfanzagl, V; Beale, JH; Michlits, H; Schmidt, D; Gabler, T; Obinger, C; Djinovic-Carugo, K; Hofbauer, S;

      Event: 33rd European Crystallographic Meeting

    • Year: 2022 -  Structural and mechanistic insights in dimeric chlorite dismutase - Impact of pH and the dynamics of the catalytic arginine

      Autoren: Schmidt, D; Serra, I; Hofbauer, S; Pfanzagl, V; Van Doorslaer, S; Obinger, C; Furtmüller, P.G.

      Event: 16th European Biological Inorganic Chemistry 2022

    • Year: 2022 -  Importance of H-bonds on substrate specificity and complex stability of firmicute coproporphyrin ferrochelatase

      Autoren: Gabler, T; Dali, A; Sebastiani, S; Helm, J; Obinger, C; Furtmüller, PG; Smulevich, G; Hofbauer, S

      Event: OxiZymes 2022

    • Year: 2021 -  Coproporphyrin ferrochelatase – study of relevant residues for substrate and product binding

      Autoren: Gabler, T; Pfanzagl, V; Obinger, C; Furtmüller PG; Hofbauer, S

      Event: International Conference on Porphyrins and Phthalocyanines - ICPP11

    • Year: 2019 -  Interaction of hydrogen sulfide with myeloperoxidase in presence of hydrogen peroxide or oxygen

      Autoren: Furtmüller, PG; Palinkas, Z; Garai, D; Rios-Gonzalez, BB; Lopez-Garriga, J; Hofbauer, S; Obinger, C; Nagy, P

      Event: 11th Human Peroxidase Meeting

    • Year: 2019 -  Resonance Raman to understand the heme cavity impact on the structure and function of coproheme decarboxylase (ChdC) from Listeria monocytogenes

      Autoren: Milazzo, L; Hofbauer, S; Gabler, T; Furtmüller, PG; Obinger, C; Smulevich, G

      Event: 10th International Conference on Advanced Vibrational Spectroscopy

    • Year: 2019 -  Posttranslational Modification of Heme in Peroxidases - Impact on Structure and Function

      Autoren: Furtmüller, PG; Nicolussi, A; Sevcnikar, B; Paumann-Page, M; Auer, M; Zamocky, M; Pfanzagl, V; Hofbauer, S; Obinger, C

      Event: 19th International Conference on Biological Inorganic Chemistry 2019

    • Year: 2019 -  YfeX - A new player involved in the redox gated potassium efflux pump KefC

      Autoren: Pfanzagl, V; Schwaiger, L; Michlits, H; Hofbauer, S; Obinger, C

      Event: Gordon Research Conference: Mechanisms of Membrane Transport

    • Year: 2019 -  Role of H98Y variant of human myoglobin in Myoglobinopathy

      Autoren: Hofbauer, S; Bernini, C; Pignataro, M; Obinger, C; Battistuzzi, G; Furtmüller, PG

      Event: 11th Human Peroxidase Meeting

    • Year: 2019 -  An actinobacterial representative sheds a new light on the reaction mechanism of coproheme decarboxylases

      Autoren: Michlits, H; Pfanzagl, V; Furtmüller, PG; Djinovic-Carugo, K; Obinger, C; Hofbauer, S

      Event: 19th International Conference on Biological Inorganic Chemistry 2019

    • Year: 2019 -  Molecular mechanism of coproheme to heme b conversion

      Autoren: Hofbauer, S; Milazzo, L; Gabler, T; Pühringer, D; Pfanzagl, V; Djinovic-Carugo, K; Furtmüller, PG; Obinger, C; Smulevich, G

      Event: 19th International Conference on Biological Inorganic Chemistry 2019

    • Year: 2019 -  Molecular Mechanism of Enzymatic Chlorite Degradation

      Autoren: Obinger, C; Schaffner, I; Schmidt, D; Smulevich, G; Coates, L; Battistuzzi, G; Furtmüller, PG; Hofbauer, S

      Event: 19th International Conference on Biological Inorganic Chemistry 2019

    • Year: 2019 -  X-ray induced redcution kinetics of heme metal centers is protein-independent - implications for structural studies of redox sensitive proteins

      Autoren: Pfanzagl, V; Beale, J; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, K; Obinger, C; Hofbauer, S

      Event: 19th International Conference on Biological Inorganic Chemistry 2019

    • Year: 2019 -  X-ray induced reduction of heme metal centers is protein-independent – implications for structural studies of redox sensitive proteins

      Autoren: Pfanzagl, V; Beale, JH; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, C; Obinger, C; Hofbauer, S

      Event: Hot Topics in Crystallography 4

    • Year: 2019 -  X-ray induced redcution kinetics of heme metal centers is protein-independent

      Autoren: Pfanzagl, V; Beale, J; Schmidt, D; Michlits, H; Gabler, T; Djinovic-Carugo, K; Obinger, C; Hofbauer, S

      Event: 11th Human Peroxidase Meeting

    • Year: 2019 -  Manipulation of heme environment in the active site of chlorite dismutases and its impact on the enzymatic activity

      Autoren: Schmidt, D; Schaffner, I; Hofbauer, S; Furtmüller PG; Obinger, C

      Event: 19th International Conference on Biological Inorganic Chemistry 2019

    • Year: 2019 -  Posttranslational modification of heme - Impact on generation of hypohalous acids

      Autoren: Obinger, C; Nicolussi, A; Auer, M; Sevcnikar, B; Paumann-Page, M; Hofbauer, S; Furtmüller, PG;

      Event: 11th Human Peroxidase Meeting

    • Year: 2018 -  Coproheme decarboxylase from Listeria monocytogenes: insight into the active site structure

      Autoren: Milazzo, L; Hofbauer, S; Howes, BD; Gabler, T; Furtmüller, PG; Obinger, C; Smulevich, G.

      Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)

    • Year: 2018 -  Characterization and comparison of the distal heme environment of dimeric and pentameric chlorite dismutases

      Autoren: Schmidt, D., Schaffner, I., Hofbauer, S., Mlynek, G., Djinović-Carugo, K., Furtmüller, P. G., Obinger, C.

      Event: OxiZymes 2018

    • Year: 2018 -  Mechanistic Studies on Coproheme Decarboxylase

      Autoren: Hofbauer, S; Milazzo, L; Mlynek, G; Gabler, T; Howes, BD; Pfanzagl, V; Djinović-Carugo, K; Furtmüller, PG; Smulevich, G; Obinger, C

      Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)

    • Year: 2018 -  Crystal and Electronic Structure of Compound I in Dye-decolorizing Peroxidases

      Autoren: Pfanzagl, V; Hofbauer, S; Beale, J; Mlynek, G; Michlits, H; Nys, K; Van Doorslaer, S; Djinovic-Carugo, K; Furtmüller, PG; Obinger, C

      Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)

    • Year: 2018 -  Kinetic and spectroscopic characterization of EfeB

      Autoren: Michlits, H., Pfanzagl, V., Schwaiger, L., Furtmüller, P. G., Hofbauer, S., Obinger, C.

      Event: OxiZymes 2018

    • Year: 2018 -  On Compound I formation in B-type dye decolorizing peroxidases - lessons from structural and kinetic studies.

      Autoren: Pfanzagl, V., Nys, K., Mlynek, G., Djinović-Carugo, K., Van Doorslaer, S., Furtmüller, P. G., Hofbauer, S., Obinger, C.

      Event: OxiZymes 2018

    • Year: 2018 -  Molecular mechanism of enzymatic chlorite degradation

      Autoren: Obinger, C; Schaffner, I; Smulevich, G; Coates, L; Battistuzzi, G; Furtmüller, P.G; Hofbauer, S;

      Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)

    • Year: 2018 -  Coproheme decarboxylases – phylogenetic prediction versus biochemical analysis.

      Autoren: Pfanzagl, V., Holcik, L., Maresch, D., Michlits, H., Furtmüller, P. G., Obinger, C., Hofbauer, S.

      Event: OxiZymes 2018

    • Year: 2018 -  Posttranslational Modification of Heme in Peroxidases – Impact on Structure and Catalysis

      Autoren: Furtmüller, PG; Nicolussi, A; Auer, M; Paumann-Page, M; Sevcnikar, B; Pfanzagl, V; Zámocký, M; Hofbauer, S; Obinger, C;

      Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)

    • Year: 2018 -  Coproheme Listeria monocytogenes HemQ and selected mutants to highlight the structure function

      Autoren: Milazzo, L; Hofbauer, S; Howes, BD; Obinger, C; Smulevich, G

      Event: Tenth International Conference on Porphyrins and Phthalocyanines (ICPP-10)

    • Year: 2017 -  How heme to protein bonds modulate reactivity - Lessons from a bacterial peroxidase with homology to lactoperoxidase

      Autoren: Furtmüller, P.G; Nicolussi, A; Auer, M; Hofbauer, S; Bellei, M; Battistuzzi, G; and Obinger, C

      Event: 10th International Human Peroxidase Meeting - Breckenridge 2017

    • Year: 2017 -  Roles of the distal Asp-Arg pair in compound I formation of K. pneumoniae dye-decolorizing peroxidase

      Autoren: Pfanzagl, V; Hofbauer, S; Nys, K; Djinović-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmüller, P.G; and Obinger, C;

      Event: International Conference on Bioinorganic Chemistry ICBIC-18 2017

    • Year: 2017 -  Pre-steady-state kinetics reveal the substrate specifity and mechanism of halide oxidation of turncated human peroxidasin

      Autoren: Paumann-Page, M; Katz, R.-S; Bellei, M; Schwartz, I; Edenhofer, E; Sevcnikar, B; Soudi, M; Hofbauer, S; Battistuzzi, G; Furtmüller, P.G; and Obinger, C;

      Event: 10th International Human Peroxidase Meeting - Breckenridge 2017

    • Year: 2017 -  Roles of the distal Asp-Arg pair in Compound I formation of K. pneumoniae dye-decolorizing peroxidase

      Autoren: Pfanzagl, V; Hofbauer, S; Nys, K; Djinovic-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmuller, PG; Obinger, C

      Event: International Conference on Bioinorganic Chemistry ICBIC-18 2017

    • Year: 2016 -  High-resultion X-ray and neutron diffration structures and biophysical studies on a novel dimeric chlorite dismutase

      Autoren: Schaffner, I., Mlynek, G., Hofbauer, S., Smulevich, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Coates, L., Obinger, C.

      Event: 79th Harden Conference on Oxygen Evolution and Reduction - Common Principles

    • Year: 2016 -  Chlorite dismutase: Combining X-ray/neutron crystallography to investigate structure-function relationships

      Autoren: Schaffner, I; Mlynek, G; Coates, L; Hofbauer, S; Djinović-Carugo, K; Furtmüller, P.G; Obinger, C

      Event: OxiZymes 2016

    • Year: 2016 -  Chlorite dismutase: distal heme environment and its role in irreversible enzyme inactivation

      Autoren: Schaffner, I., Hofbauer, S., Mlynek, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Obinger, C.

      Event: 13th European Biological Inorganic Chemistry Conference EuroBIC 2016

    • Year: 2016 -  Dye decolorizing peroxidases - A new heme-peroxidase family with ancient roots

      Autoren: Pfanzagl, V; Schaffner, I; Hofbauer, S; Furtmuller, P; Obinger, C

      Event: European Congress on Biotechnology 2016

    • Year: 2016 -  Dioxygen evolution by chlorite dismutase depends on co-ordination of transiently produced hypochlorite to an oxoiron(IV) intermediate

      Autoren: Hofbauer, S., Grünwald-Gruber, C., Schaffner, I., Sündermann, A., Jakopitsch, C., Oostenbrink, C., Djinovic-Carugo, K., Furtmüller, P.G., Obinger, C.

      Event: 79th Harden Conference on Oxygen Evolution and Reduction

    • Year: 2016 -  Dye decolorizing peroxidases – a new heme-peroxidase family with ancient roots

      Autoren: Pfanzagl, V; Schaffner, I; Hofbauer, S; Furtmüller, P.G; Obinger, C

      Event: 17th European Congress on Biotechnology ECB17 2016

    • Year: 2016 -  Coproheme and HemQ

      Autoren: Hofbauer, S., Scheiblbrandner, S., Schaffner, I., Djinović-Carugo, K., Battistuzzi, G., Furtmüller, P. G., Obinger, C.

      Event: 13th European Biological Inorganic Chemistry Conference EuroBIC 2016

    • Year: 2014 -  Mechanistic studies on a new functional dimeric chlorite dismutase

      Autoren: Schaffner, I; Flego, N; Mlynek, G; Bellei, M; Hofbauer, S; Battistuzzi, G; Djinovic-Carugo, K; Smulevich, G; Furtmuller, PG; Obinger, C

      Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014

    • Year: 2014 -  Mechanism of reaction of chlorite with mammalian peroxidases

      Autoren: Jakopitsch, C; Pirker, KF; Flemmig, J; Hofbauer, S; Schlorke, D; Furtmuller, PG; Arnhold, J; Obinger, C

      Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014

    • Year: 2014 -  Chlorite inactivates mammalian peroxidases

      Autoren: Jakopitsch, C., Pirker, K.F., Flemmig, J. Hofbauer, S., Furtmüller, P.G., Schlorke, D., Arnhold, J., Obinger, C.

      Event: OxiZymes 2014

    • Year: 2014 -  Biochemical and biophysical characterization of a new functional chlorite dismutase

      Autoren: Schaffner, I., Hofbauer, S., Pirker, K.F., Furtmüller, P.G., Obinger, C.

      Event: OxiZymes 2014

    • Year: 2014 -  Understanding the inhibition mechanism of chlorite dismutase from the nitrite-oxidizing bacterium Candidatus Nitrospira defluvii

      Autoren: Hofbauer, S; Gruber, C; Schaffner, I; Pirker, KF; Jakopitsch, C; Sundermann, A; Oostenbrink, C; Furtmuller, PG; Obinger, C

      Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014

    • Year: 2014 -  Chlorite to chloride and O2 conversion: new lessons from structural and mechanistic investigations of chlorite dismutase

      Autoren: Obinger, C., Hofbauer, S., Schaffner, I., Pirker, K. F., Mlynek, G.,Djinovic-Carugo, K., Battistuzzi, G., Furtmüller, P. G

      Event: 12th European Biological Inorganic Chemistry Conference EuroBIC 2014

    • Year: 2014 -  Redox properties of the Fe3+/Fe2+ couple in wild-type and mutated heme chlorite dismutases

      Autoren: Hofbauer, S., Bellei, M., Schaffner, I., Furtmüller, P.G., Obinger, C., Battistuzzi, C.

      Event: OxiZymes 2014

    • Year: 2014 -  Characterization of the Major Peptidoglycan Hydrolase of Lactobacillus buchneri

      Autoren: Anzengruber, J; Courtin, P; Pabst, M; Neumann, L; Claes IJJ; Debreczeny, M; Hofbauer, S; Obinger, C; Altmann, F; Chapot-Chartier, M-P; Vanderleyden, J; Messner, P; Schäffer, C

      Event: FASEB Science Research Conference on Microbial Glycobiology

    • Year: 2014 -  Chlorite dismutases and dye-decolorizing peroxidases – similarities and differences within a structural superfamily of heme proteins

      Autoren: Schaffner, I., Hofbauer, S., Furtmueller, P. G., Obinger, C.

      Event: 16th European Congress on Biotechnology 2014

    • Year: 2014 -  Mechanism of inhibition of chlorite dismutase

      Autoren: Hofbauer, S., Schaffner, I., Gruber, C., Pirker, K.F., Jakopitsch, C., Sündermann, A., Oostenbrink, C., Furtmüller, P.G., Obinger, C.

      Event: OxiZymes 2014

    • Year: 2013 -  New highly stable chlorinating bacterial peroxidase with autocatalytically formed covalent heme to protein bonds

      Autoren: Furtmuller, PG; Auer, M; Gruber, C; Pirker, K; Krioss, D; Hofbauer, S; Soudi, M; Obinger, C; Zamocky, M; Bellei, M; Battistuzzi, G

      Event: International Conference on Bioinorganic Chemistry ICBIC-16 2013

    • Year: 2013 -  Understanding Chlorite Dismutase from Candidatus Nitrospira defluvii

      Autoren: Hofbauer, S; Gruber, C; Pirker, KF; Schaffner, I; Furtmuller, PG; Obinger, C; Hagmuller, A; Gysel, K; Mlynek, G; Kostan, J; Djinovic-Carugo, K; Bellei, M; Battistuzzi, G; Daims, H

      Event: International Conference on Bioinorganic Chemistry ICBIC-16 2013

    • Year: 2013 -  Spectroelectrochemical characterization of heme enzymes

      Autoren: Bellei, M., Hofbauer, S., Furtmüller, P. G., Obinger, C., Battistuzzi, G.

      Event: International workshop on protein electron transfer: from fundamentals to applications for health

    • Year: 2013 -  Understanding highly stable chlorite dismutase form nitrate-oxidizing bacterium Candidatus “Nitrospira defluvii”

      Autoren: Hofbauer, S., Gruber, C., Pirker, K. F., Schaffner, I., Hagmüller, A., Gysel, K., Mlynek, G., Kostan, J., Bellei, M., Daims, H., Djinovic-Carugo, K. Battistuzzi, G., Furtmüller, P.G. and Obinger, C

      Event: International Conference on Bioinorganic Chemistry ICBIC-16 2013

    • Year: 2013 -  Spectroscopic Characterization of wild-type Chlorite Dismutase from Candidatus “Nitrospira defluvii” and selected variants

      Autoren: Hofbauer, S., Flego, N., Schaffner, I., Pirker, K. F., Furtmüller, P. G., Howes, B. D., Obinger, C., Smulevich, G.

      Event: 15th European Conference on the Spectroscopy of Biological Molecules

    • Year: 2012 -  Redox theromdynamics of diverse chlorite dismutases

      Autoren: Hofbauer, S. Bellei, M. Sündermann, A. Kostan, J. Daims, H. Djinović-Carugo, K. Oostenbrink, C. Furtmüller, P.G. Battistuzzi, G. & Obinger, C.

      Event: OxiZymes 2012

    • Year: 2012 -  Thermal and Conformational Stability of Pentameric Chlorite Dismutase from Candidatus Nitrospira defluvii

      Autoren: Hofbauer, S., Gysel, K., Mlynek, G., Kostan, J., Daims, H., Furtmüller, P.G., Djinovics-Carugo, K. and Obinger,C.

      Event: 9th International Conference on Protein Stability 2012 - From molecular level to market applications

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