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Gewählte Doctoral Thesis:

Apanee Pokeprasert (2006): Application of SSR Markers to Identify QTL and Epistasis in Seed Characters of Soybean.
Doctoral Thesis, BOKU-Universität für Bodenkultur. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
Soybean [Glycine max (L.) Merr.] is now a dominant source of protein and oil for human food and animal feed. Enhanced seed quality has become an important objective in soybean breeding. The objectives of this study were to identify quantitative trait loci (QTLs) and epistatic gene interaction (epistasis) affecting 1000-seed weight (TSW), protein concentration (PRO), oil concentration (OIL), and sum of protein plus oil concentration (PO) in the population from a cross of the two early maturity cultivars Ma.Belle and Proto. An F2 population consisting of 82 individuals had initially been mapped with a large number of simple sequence repeat (SSR) markers. In the F6 generation, 530 individual plants were randomly taken from 82 F2 derived lines. An F2:6:7 population consisting of 530 recombinant inbred lines was grown and phenotypically evaluated in two replications at four environments (Raasdorf, Austria). 530 F2:6:10-derived lines were regenotyped at 35 SSR marker loci, which were associated with QTLs in the initial analysis. Based on single-factor ANOVA, 17 QTLs associated with TSW, 11 for PRO, 6 for OIL and 10 for PO were detected. Most markers associated with seed trait QTLs were stable across environments. These QTLs could be useful in soybean breeding programs for selection of soybeans with desirable traits through marker-assisted selection. Using two-way ANOVAs, 19 epistatic gene interactions were detected for TSW, 23 for PRO, but only 2 for OIL and 4 for PO. Selection for increased PRO was associated with decreased OIL and increased TSW. The present study indicates that soybean breeders interested in enhancing complex phenotypes such as seed weight or seed protein content may need to consider both single QTL effects and epistasis.


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