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Gewählte Master / Diploma Thesis:

Maria Fidesser (2013): Molekulare Detektion der Lactobacillendiversität in humanen Habitaten.
Master / Diploma Thesis - Institut für Lebensmittelwissenschaften, BOKU-Universität für Bodenkultur, pp 188. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
The actually available scientific data only partly describes the vaginal Lactobacillus species spectrum of healthy pregnant women. Due to very different analysis technologies applied, the published data cannot be compared directly. Within this master thesis the vaginal Lactobacillus microflora was analyzed in detail by applying a culture-independent molecular technique. This allowed the detection of species witch are very difficult to cultivate (e.g. Lactobacillus iners). In detail, based on the approval of the ethical committee of the University Hospital of Vienna (AKH) vaginal and rectal smears from 100 healthy pregnant women were taken. These samples were then analyzed by the culture-independent PCR/DGGE technique, applying primers specific for the Genus Lactobacillus targeting the V3 region of the 16S rDNA. The Lactobacillus spectrum of the samples was derived from the banding patterns of the DGGE gel and additionally sequencing of selected bands. Further the samples were screened for L. iners by species-specific PCR-based techniques. The results show that L. crispatus and L. iners dominate the rectal and vaginal Lactobacillus microflora of healthy pregnant women. This Lactobacillus spectrum corresponds to the Lactobacillus microflora of non-pregnant women in the reproductive age. It could be shown that the individual Lactobacillus species microflora could be successfully identified by PCR/DGGE technique. The species-specific PCR-based detection of Lactobacillus species allows a more sensitive detection, which was shown by the specific detection of L. iners.

Beurteilende(r): Kneifel Wolfgang
1.Mitwirkender: Domig Konrad

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