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Gewählte Master / Diploma Thesis:

Master / Diploma Thesis - Institut für Angewandte Mikrobiologie (IAM), BOKU-Universität für Bodenkultur, pp 85. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Articular cartilage repair remains one of the most intensely studied orthopaedic topics. A promising future for the repair of damaged articular cartilage lies in the tissue engineering approach. The ideal cell source for cartilage tissue engineering would be one that can easily be accessed, isolated, expanded and differentiated towards the chondrogenic lineage, like human mesenchymal stem cells (hMSCs). There are various sources for hMSCs, including bone marrow tissue, umbilical cord and adipose tissue. Especially adipose tissue has received much attention because it is ethical without any severe concerns and abundantly accessible in course of most bariatric, abdominoplastic and liposuction surgeries. In vivo articular cartilage cells are exposed to mechanical forces due to joint movement. To better reflect the natural three-dimensional microenvironment, dynamic cultivations of hMSCs on biomaterials are performed, applying different bioreactor systems, to optimize the yield and quality of in vitro expanded cartilage constructs. In this regard, two different objectives were investigated in this thesis. First of all, the influence of oxygen tension on the static cultivation of adipose tissue derived mesenchymal stem cells (adMSCs) was explored. And second, a tailor-made bioreactor system in special focus on its dynamic cultivation factors, including fluid flow induced shear stress and scaffold porosity, was evaluated. Cultivation of adMSCs in hypoxic conditions (5% O2) was performed and cell proliferation, metabolic activity, differentiation capacity were studied. Hypoxic conditions caused an increase in the proliferation activity and a higher degree of differentiation of adMSCs. Evaluation of dynamic cultivation characteristics showed that this bioreactor system is a potent tool for cartilage tissue engineering. However, the data suggested, that Matristypt is a non-suitable matrix for dynamic cultivation in this particular perfusion bioreactor system.

Beurteilende(r): Kasper Cornelia
1.Mitwirkender: Obinger Christian

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