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Gewählte Master / Diploma Thesis:

Stefan Aigner (2013): Antimikrobielle Empfindlichkeit von indigenen Milchsäurebakterien isoliert aus montenegrinischem Käse.
Master / Diploma Thesis - Institut für Lebensmittelwissenschaften, BOKU-Universität für Bodenkultur, pp 174. UB BOKU obvsg

Data Source: ZID Abstracts
The aim of this work was to isolate lactic acid bacteria, especially Lactobacillus spp. and Leuconostoc spp., from 21 Montenegrin cheese samples. At the same time, these cheese samples were investigated using cultural methods for the presence of the pathogenic microorganisms Listeria monocytogenes and Staphylococcus aureus as well as for Enterobacteriaceae and E. coli. Additionally, the presence of Listeria monocytogenes and Listeria spp. was tested by applying the VIDAS-LDUO system. The identification of the isolates at species level was done using molecular biological methods with subsequent 16S rDNA sequencing. The results showed a broad spectrum of lactobacilli and Leuconostoc. Corresponding to their colony morphology and appearance, Staphylococcus aureus, Enterobacteriaceae and E. coli could be detected as well. However, presumptive Staphylococcus aureus and Escherichia coli isolates were attributed to other species by sequencing. Furthermore, antimicrobial susceptibility testing was performed with 246 enterococci. Enterococci are Gram-positive, facultative anaerobic bacteria, which naturally occur in the gastrointestinal tract of humans and animals. They are significantly involved in the ripening process of many cheese types in Southern Europe. Nevertheless, this group of bacteria within the lactic acid bacteria contains strains, witch due to their ability to cause infections and to transfer antibiotic resistance genes cause a risk. The applied methods were agar disc diffusion and microdilution techniques. According to the FEEDAP-Panel of the EFSA, ten different antibiotics were considered. The results of both applied methods fitted well together. Particularly the strains were resistant to clindamycin, erythromycin, kanamycin, streptomycin and tetracycline. Finally, a commercially available microarray-assay was applied for the genotypic detection of resistance genes in selected enterococcal isolates.

Beurteilende(r): Kneifel Wolfgang
1.Mitwirkender: Domig Konrad

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