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Gewählte Master / Diploma Thesis:

Vera Lachmann (2014): Optimierung der viability-qPCR in der Trinkwasseranalytik am Beispiel von Enterococcus faecalis.
Master / Diploma Thesis - Institut für Lebensmittelwissenschaften, BOKU-Universität für Bodenkultur, pp 98. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
The long existing cultural ISO-method is an established and proven equipment for the detection of faecal bacteria in drinking water. Indeed, it’s a cost-efficient and uncomplicated technique, but it is very time-consuming. In conjunction with contaminating drinking water this fact plays a decisive part. Referring to the human health method-developments for rapid detection of dangerous microorganisms are extremely necessary. Therefore, nowadays more sensitive and faster molecular detection-systems become more important. The aim of this Master Thesis is the optimisation of viability-quantitative PCR-method for the detection of Enterococcus faecalis. Furthermore, the detection limit for this kind of bacteria in drinking water is to be determined by experimental tests. The chosen technology is based on sample treatment with the DNA-intercalating dye, which calls propidium monoazide (PMA). This dye only penetrates membrane compromised cells and get into a binding with the DNA. Due to the irreversible DNA modification, a differentiation between live and dead cells is available. There is no signal detectable while the PCR-test, because PMA inhibits the amplification of membrane compromised DNA.

Beurteilende(r): Kneifel Wolfgang
1.Mitwirkender: Domig Konrad

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