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Gewählte Master / Diploma Thesis:

Barbara Wachtler (1998): Investigations of cell capture mechanisms for the development of microbial sensors/ Untersuchung der Immobilisierung von Bakterien zur Entwicklung mikrobieller Sensoren.
Master / Diploma Thesis, BOKU-Universität für Bodenkultur. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
A new type of biosensor for use in combination with an automated flow injection analysis (FIA) system for on-line determination of metabolic activity, number and viability of microorganisms during their cultivation should be developed. The method works on a mediation principle on the basis of ferricyanide reductase activity. The cells reversibly bound to the surface of the sensor by immobilized lectins reduce the mediator potassium ferricyanide, which reconversion by the electrode is amperometrically measured and related to quantity and physiological activity of the cells. The biosensor can be regenerated by carbohydrates that compete with the microorganisms for binding sites. The conditions for lectin immobilization on negatively-charged surfaces of carbon paste electrodes (CPEs) with the help of the coupling reagents 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (S-NHS) were optimized. The methods for cell capture and cell release by N-acetyl-amine-sugars were also examined. The techniques used for all investigations were chronoamperometry, chronocoulometry and cyclic voltammetry.

Beurteilende(r): Bayer Karl

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