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Gewählte Master / Diploma Thesis:

Monira Awad (2015): Strategies in adipo-derived mesenchymal stem cell expansion.
Master / Diploma Thesis - Institut für Biotechnologie, BOKU-Universität für Bodenkultur, pp 137. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Due to the high medical relevance of AD-MSC (adipo-derived mesenchymal stem cells), it is important to develop ex vivo cultivation strategies that allow optimal cell yield. At this point, both the number of cells which can be achieved and the quality of the cultured cells is critical. The aim of this work was to accomplish a contribution to the optimization of AD-MSC expansion strategies. For this purpose this study was separated into two parts in order to optimize the quality as well as the yield of ex vivo expanded AD-MSC. The first strategy explores the different effects of digestive enzymes and thermoresponsible surfaces to investigate the proliferation capacities on a static culture. Furthermore, several advantages and disadvantages of long-term cultivation of AD-MSC under hypoxic (5% O2) and normoxic (21% O2) conditions were investigated. This involves the expansion capacity, the differentiation potential towards cell types (adipocytes, chondrocytes and osteoblasts) and the investigation of characteristic stem cell surface markers to guarantee the existence of such AD-MSC, based on static expansion. The second strategy focuses on the dynamic expansion of AD-MSC in a Z®RP 2000 H rotating bioreactor under hypoxic condition (5% oxygen tension) and in a disposable 3D bioreactor system under normoxic (21% O2) condition. Both expansions are based on dynamic processes. Hypoxic condition was used because of the ability of AD-MSC to survive in a milieu after transplantation and that the functions relating to the local tissue requirements can be investigated.

Beurteilende(r): Kasper Cornelia

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