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Gewählte Master / Diploma Thesis:

Barbara Marie-Therese Becede (2016): Antibiotic-resistant E. coli in the food chain - genetic basis and growth behaviour.
Master / Diploma Thesis - Institut für Lebensmittelwissenschaften, BOKU-Universität für Bodenkultur, pp 179. UB BOKU obvsg FullText

Data Source: ZID Abstracts
The discovery of antibiotics revolutionized the treatment of infectious diseases worldwide. As a consequence they were immediately applied in a broad range in human and veterinary medicine. This extensive use of antibiotics favoured the emergence and selection of antibiotic resistances, resulting in the urgent search for alternatives. In the frame of this work E. coli isolates originating from chicken and pig were examined for the presence of antibiotic resistances. The chicken population consisting of three subgroups (standard feed, feed with added antibiotics and feed with an alternative feeding supplement) was sampled during a feeding trial. In total, 240 E. coli isolates originated from poultry intestine and five from an environmental sample. Furthermore, 101 E. coli isolates from pig feces, sampled at three different time points, were tested. A genotypic investigation of the chicken intestinal isolates was performed to examine their antibiotic resistances. Furthermore, the influence of the tested feeding supplement on the growth behaviour of selected isolates was assessed by microbial growth analyses. A disc diffusion-test was conducted with the E. coli isolates from pig feces to analyse their resistances against representatives of the most commonly used antibiotic classes. The in vitro growth analyses of the chosen chicken isolates did not show any inhibition of the resistant E. coli by the use of the tested feeding supplement. The phenotypically determined antibiotic resistances were generally confirmed by the molecular biological results. A frequent appearance of antibiotic resistances was observed for the phenotypically tested E. coli isolates gained from pig feces.

Beurteilende(r): Domig Konrad
1.Mitwirkender: Mayrhofer Sigrid

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