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Gewählte Master / Diploma Thesis:

Elisabetta De Vito-Francesco (2018): Toxicological effects of Cd-containing Quantum Dots on selected mammalian cell lines.
Master / Diploma Thesis - Institut für Siedlungswasserbau, Industriewasserwirtschaft und Gewässerschutz (SIG), BOKU-Universität für Bodenkultur, pp 146. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Quantum dots (QDs) are an engineered nanomaterial-type within the size range of 1 – 10 nm, exhibiting semiconducting and fluorescent properties, and higher reactivity than the bulk counterparts. Despite the wide applications (e.g. bioimaging, drug delivery, components in electronic devices), little is known about QDs toxicological effects and standardised toxicity methods. This research focused on the assessment of potential adverse effects of four types of NAC-coated Cd-based QDs (CdTe, CdTe/ZnS, CdTe/30%Fe:ZnS, 30%Fe:CdTe/ZnS) using in vitro bioassays, while attempting to develop a fast-screening and reproducible method to assess cytotoxicity by flow cytometry analysis. CHO cells (1×106 cells/mL) were incubated with NAC-CdTe (100 µg/mL) for 24h at 37°C. However, overlapping spectra of the viability determination stain (7AAD, Hoechst and PI) and of the QD’s characteristic emission may have occurred. Therefore, cell viability assessment were proceeded via the AlamarBlue® assay, showing the dose-response behaviour. Macrophages U937 (5×105 cells/mL) were incubated with QDs (concentrations between 0.05 – 100 µg/mL), CdCl2 and FeCl2 solutions (0.3 – 30 µg/mL, 0.12 – 12 µg/mL, respectively), at 37°C for 0, 2, 4, 20, 24 hours. Due to lower half inhibition concentration value (IC50) for dissolved Cd ions (21.5 µg/mL) compared to these of QDs (66.6 µg/mL in average), and to viability reduction posed by Fe II counterparts (at 12 µg/mL), the release of these ions from the QD cores and the uptake into the cells was assumed as possible toxicity trigger factor. The images from the fluorescent microscopy analysis showed internalized QDs by the macrophages at a very low concentration (1 µg/mL) at which no significant viability decrease was observed. In conclusion, further tests are needed to better understand uptake mechanisms of QDs into cells, toxicity trigger factors and possible differences in QD types and their influence on cells.

Beurteilende(r): Fürhacker Maria
1.Mitwirkender: Part Florian

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