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Gewählte Master / Diploma Thesis:

Johannes Tichy (2020): Detection of microbial VOCs from filamentous hyphomycetes.
Master / Diploma Thesis - Institut für Mikrobiologie und Mikrobielle Biotechnologie, BOKU-Universität für Bodenkultur, pp 144. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
The topic of this Diploma-Thesis deals with the detection of microbial volatile organic compounds (MVOCs) from filamentous hyphomycetes. The aim was to differentiate single fungal species through a sum-parameter, which originates from fungi emission-patterns. Czapek-Dox agar is used as minimal-media. The used measurement installation is exclusively designed for these experiments with the ability of managing two different airways: 1. fresh-air mode & 2. air-recirculation mode. The carrier gas is pure, hydrocarbon-free and pre-humidified air. Two different commercially available metal-oxide sensors (MOS) and one combined temperature- and humidity-sensor is used. The MOS-sensors are prosecuted in a temperature-cycled operation (TCO). The sensor-signals and the extracted measurement curve features are processed through a linear-discriminant-analysis (LDA). The previously obtained results were subjected to a classification using the k-nearest-neighbour (knn) algorithm with an additional leave-one-out cross-validation (LOOCV). The resulting numerical operative separation performance of the specific emission patterns depending on fungal strain and aeration mode varies between 84-97%.

Beurteilende(r): Sterflinger-Gleixner Katja

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