University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Gewählte Master / Diploma Thesis:
Katharina Neubacher
(2020):
Identifikation des Thrips tabaci Komplexes in Österreich und Ungarn.
Master / Diploma Thesis - Institut für Pflanzenschutz,
BOKU-Universität für Bodenkultur,
pp 70.
UB BOKU
obvsg
- Abstract:
- Thrips tabaci Lindeman is known as a pest of vegetables and horticultural crops in the field as well as in the greenhouse and as a vector of tomato spotted wilt virus (TSVW) and iris yellow spot virus (IYSV). The T. tabaci species complex includes three genetic lineages (lineage L1, lineage L2, lineage T) with differences in host plant preference and reproduction mode. For the present master thesis T. tabaci individuals, which were collected on onion crops in Austria and Hungary, were analysed morphologically and molecular biologically in order to determine the distribution of the three genetic lineages of T. tabaci. The morphological identification of slide mounted T. tabaci abdomen was performed with 40-fold magnification under the transmitted-light microscope. A proportion of 97% of the samples could successfully be determined morphologically as T. tabaci. Subsequently, the molecular biological analysis of head/thorax of the samples already determined as T. tabaci was performed. Based on an refined identification protocol 209 of 228 thrips from Austria and Hungary were successfully identified by molecular biology and assigned to the three genetic lineages of T. tabaci. The molecular identification of two larvae also proved to be successful. The total proportion of T. tabaci lineage L1 was 28%. Lineage L2 prevailed with a proportion of 63% of the samples. Two Hungarian individuals could be assigned to lineage T. Differences in the temporal distribution of the three genetic lineages could be shown in Hungary, in Austria this was not possible due to the one time sampling per site. Differences in the spatial distribution of the genetic lineages of T. tabaci could be shown in Austria and Hungary. The detection of sympatric populations of T. tabaci on onion was successful on all Austrian sites. The Hungarian samples also showed the synchronous occurrence of different reproductive modes of T. tabaci on the same site. The modified identification protocol, which is shown in the present master thesis, will be of importance as a basis for further studies.
- Beurteilende(r): Koschier Elisabeth Helene