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Gewählte Doctoral Thesis:

Jill Molsner (2017): Gameto-maternale Interaktion im bovinen Ovidukt: In-vitro-Untersuchungen zum Transport des Kumulus-Oozyt-Komplexes durch die Eileiterampulle.
Doctoral Thesis - Institut für Nutztierwissenschaften (NUWI), BOKU-Universität für Bodenkultur, pp 110. UB BOKU obvsg FullText

Abstract:

The aim of this study was to analyse the transport of the cumulus-oocyte-complex (COC) through the ampulla section of the bovine oviduct and its dependence on the sexual cycle stage of the cow, the presence of follicular fluid and the stage of maturation of the oocyte. Additionally, the contact between the COC and the oviductal epithelium was assessed. Bovine samples were gained in an Austrian slaughterhouse. Light microscopic inspection of the bond between the immature COC (n=20), in-vitro-matured COC (n=20) and the oviductal epithelium was performed using histochemical stainings. The ultrastructure of the bond (n=14) was characterised using a transmission electronic microscope; furthermore glycogen was detected (n=3). The effect of follicular fluid and COC on the transport in the oviduct was assessed in terms of particle transport speed (n=86) and the ciliary beating frequency (n=20) using a digital videomicroscope. After entering the ampulla, the COC forms a bond with the epithelium. The basal particle transport speed averaged 140 µm/s. Cyclic alterations were not monitored (p=0,636). The application of follicular fluid (p=0,015) and in combination with matured (p<0,001) and immature COC (p<0,001) reduced particle transport speed significantly. The basal ciliary beating frequency averaged 15,7 Hz. After the application of follicular fluid, ciliary beating increased statistically weak (p=0,031). Evaluation of the ultrastructural and histological data showed the following results: Both immature and matured COC form an attachment involving the cumulus cells and the kinocilia of the epithelium. In-vitro and in-vivo matured COC form a firm bond with the epithelium by the extracellular matrix. Immature cumulus cells contain a large amount of glycogen, which decreases during the maturation of the oocyte. Incubation of the oviduct with immature und matured COC did not alter the secretion of the epithelium. However, there are differences due to cyclic changes.

Betreuer: Winckler Christoph