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Gewählte Master / Diploma Thesis:

Andreas Schedl (2013): ANALYSIS OF VOLATILE WOUND EXUDATES.
Master / Diploma Thesis - Abteilung für Chemie nachwachsender Rohstoffe, BOKU-Universität für Bodenkultur, pp 62. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
Wound odor is a problem in modern society and is currently not appropriately addressed. Wound odor can be caused by necrosis and nonsporing anaerobic bacteria that colonize skin wounds. Also tissue degradation and death of tissue layers release compounds such as short chain fatty acids, sulphuric compounds and diamines causing malodor. Aim of this work was the development of a comparative test method for novel types of wound dressings. In order to analyze the volatile compounds a gas chromatograph linked to a mass sensitive detector (GC-MS) was used for separation and identification. Selective Ion Monitoring (SIM) based GC–MS methods after extraction of volatile organic compounds with head-space solid phase microextraction (HS-SPME) were developed for odor standards like butyric acid, isobutyric acid, valeric acid, isovaleric acid, dimethyl trisulfide, putrescine and cadaverine. SPME was carried out with different fiber types for a faster and solvent-free extraction of the headspace of the samples and a lesser need for preparation time. Full-scan mode was used to obtain the optimal gas chromatographic conditions, retention times and target ion masses for quantification and qualification of each compound. The methods developed enable a direct comparison of wound dressings for their respective field of use.

Beurteilende(r): Potthast Antje

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