University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Gewählte Master / Diploma Thesis:
Simon Reinhold Dürr
(2014):
Development of high-throughput methods for the detection of hydrogen cyanide-producing bacteria for the application in biocontrol.
Master / Diploma Thesis - Institut für Bodenforschung (IBF),
BOKU-Universität für Bodenkultur,
pp 148.
UB BOKU
obvsg
FullText
- Abstract:
- Hydrogen cyanide-producing bacteria have been tested for their potential use as biocontrol agents for several decades now. Nevertheless, there is still a lack of fast and reliable high-throughput tests for qualitative detection of cyanogenic bacteria. Standard methods described in the literature are either too cumbersome or imprecise and error-prone. In the master’s thesis at hand, we present several tests for rapid large-scale screening of hydrogen cyanide-producing bacteria. Distinct functional and molecular methods have been developed to investigate bacterial isolates as well as metagenomic libraries. We expect these assays to accelerate and facilitate research of bacterial cyanogenesis. Seven cyanogenic Pseudomonas isolates detected with our new tests were found to significantly improve the germination of the model plant lettuce. These plant growth-promoting bacteria could be of interest for biocontrol. Additionally, an unknown volatile compound was discovered with a function-based screening for hydrogen cyanide-producing clones of a rhizosphere cosmid metagenomic library. So far, activity-driven tests and Next-Generation Sequencing allowed to identify some of the functions and genes possibly involved in the production of this volatile.
- Beurteilende(r):