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Gewählte Doctoral Thesis:

Christina Bliem (2017): Development of miniaturized sensors for the detection of clinically relevant analytes based on ion selective electrodes and reduced graphene oxide field effect transistors.
Doctoral Thesis - Department für Nanobiotechnologie (DNBT), BOKU-Universität für Bodenkultur, pp 87. UB BOKU obvsg

Data Source: ZID Abstracts
Electrochemical sensors present a powerful tool for real-time measurement of a variety of analytes for a wide range of applications such as food safety, environmental monitoring, drug screening and diagnosis. Point-of-care devices which can be used at patient’s bedside or for online monitoring of critical parameters are therefor of great importance in clinical and pharmaceutical daily routine. In this work portable, low cost electrochemical sensors have been developed for a fast and reliable detection of the clinically relevant analytes serotonin and urea. A novel all solid state ion selective electrode (ISE) for the potentiometric detection of the neurotransmitter serotonin was developed by incorporating an ion pair complex between the analyte and a carborane anion acting as ionophore, into a plasticized PVC membrane. The miniaturized electrode was optimized leading to a near Nernstian response and a limit of detection in the µ-molar range. Enzymatic-polyelectrolyte multilayer assemblies on reduced graphene oxide (rGO) based field effect transistors (FETs) were used for the detection of urea. The intrinsic properties of the developed rGO FETs could be exploited to detect the enzymatic conversion of urea in a range of 1-1000 µM with a LOD down to 1 µM. Furthermore, the inhibition of urease by heavy metal ions was exploited to detect Cu2+ at a LOD of 10 nM.

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