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Gewählte Master / Diploma Thesis:

Christoph Wachholder (2016): High-Performance Thin-Layer Chromatography of Wood Derived Sugars: Method Development and Validation.
Master / Diploma Thesis - Abteilung für Chemie nachwachsender Rohstoffe, BOKU-Universität für Bodenkultur, pp 76. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Great efforts are put in the replacement of petrochemicals with bio-based ones gained in biorefineries. For an optimal process control, lignocellulosic biorefinery concepts require fast and accurate analytical methods for mixtures of carbohydrates derived from industrial processes. In this work high-performance thin-layer chromatography (HPTLC) was tested as an alternative system to analyse carbohydrates in more complex mixtures of hydrolysed lignocelluloses. The method presented in this work provides a useful tool of analysing carbohydrates in a rapid, precise and accurate way. It was carefully developed allowing a complete separation of glucose, galactose, mannose, arabinose, xylose and cellobiose. The method was validated regarding selectivity, accuracy, precision, robustness, limit of detection and limit of quantification. In a third step, industrial samples were tested showing a reliable qualitative and quantitative analysis. The procedure of acid hydrolysis was modified to achieve a complete degradation of lignocellulose, as current standard methods applicable to HPTLC do not fully convert oligosaccharides into its monomeric compounds. This work showed that high-performance thin-layer chromatography does not only complement other chromatographic methods, but is a reliable and fully functional analytical stand-alone method of detecting carbohydrates both in a qualitative and quantitative way.

Beurteilende(r): Potthast Antje
1.Mitwirkender: Oberlerchner Josua Timotheus

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