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Gewählte Master / Diploma Thesis:

Barbara Eva Maria Bachmann (2017): Cartilage-on-a-chip Recreating functional cartilage microtissues using microfluidic devices.
Master / Diploma Thesis - Department für Nanobiotechnologie (DNBT), BOKU-Universität für Bodenkultur, pp 95. UB BOKU obvsg

Data Source: ZID Abstracts
In vitro cell culture systems for drug testing have recently come into focus for their ability of improving reliability and reducing animal testing. One of the most promising approaches are organs-on-chips, cell-based assays incorporating functional microtissues inside microfluidic devices. Despite efforts for establishing such an organ-on-a-chip for recreation of cartilage microtissues, the technology is still in its infancy. For successful engineering of a functional cartilage-on-chip, microfluidics needs to be combined with three dimensional cultivation of chondrocytes to accurately and reliably mimic the bodily environment. Articular cartilage is characterized by four distinct key characteristics – relative acellularity, avascularity, low metabolic activity and structural organization. A functional cartilage-on-chip mimicking these key characteristics helps to gain a deeper understanding of the cellular responses propagating the chronic inflammatory processes in osteoarthritis. In this work a microfluidic device was designed, fabricated and tested including device optimization, integration of complex biology using primary equine chondrocytes embedded within a fibrin hydrogel as biological model and investigation of viability, morphology, metabolic activity and gene expression. Using the optimized microfluidic design, over 95% of the cultivated chondrocytes maintained a physiological morphology and high viability throughout the culture period. The metabolic activity was significantly lower compared to monolayer chondrocytes, gene expression analysis showed elevated levels of cartilage related genes and fluorescence imaging indicated a spontaneous structural alignment of the chondrocytes, confirming the similarities between the cartilage microtissue and articular cartilage. These results prove the suitability of the engineered cartilage-on-a-chip and present the first step in the development of a reproducible human model for osteoarthritis.

1.Mitwirkender: Kasper Cornelia

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