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Gewählte Master / Diploma Thesis:

Nataša Sarić (2018): Understanding the Microbiota of Potato Tubers.
Master / Diploma Thesis - Institut für Bodenforschung (IBF), BOKU-Universität für Bodenkultur, pp 59. UB BOKU obvsg

Data Source: ZID Abstracts
The diverse community of microorganisms colonizing plants, known as the plant microbiota, gained interest for its potential to benefit sustainable agriculture. It consists of epiphytes and endophytes, living on and inside plant tissues, respectively. Because of their intimate relationship to their host endophytic bacteria might be of special interest. They may increase nutrient acquisition, promote health or even decrease post-harvest losses. Such losses, mainly due to sprouting, play a central role in the warehousing of potatoes. Thereby long-term storage and by this the necessity to control sprouting become indispensable. One possibility is to take advantage of naturally occurring plant-microbe interactions, in order to substitute chemical-synthetic growth regulators. According to that, this work aimed to deliver a first insight into the cultivable bacterial community of potato tubers. Moreover, possible coherences between the tubers sprouting behavior and their microbiota were examined. In addition, the microbiota within tuber parts of the same varieties, as well as differences between different varieties were studied. This was achieved by the isolation and identification of cultivable bacteria from two segments of potato tubers of five different varieties. Altogether 400 strains were isolated and identified, based on their 16S rRNA gene sequences, up to genus level. All strains were classified into the four phyla Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. They belonged to 42 different genera, out of which only four (Arthrobacter, Bacillus, Rhodococcus and Staphylococcus) occurred in all five varieties, representing the core microbiota of cultivable bacteria. Based on the data from this study, no direct correlation between the cultivable microbiota of potato tubers and the sprouting behavior of the varieties could be determined. Finally, a lower generic richness was observed within the inner tuber parts, compared to the outer ones.


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