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Gewählte Doctoral Thesis:

Simone Hageneder (2021): Polymer biointerfaces for plasmonic nanostructures enabling fast, multiplexed, and sensitive detection of biomarkers.
Doctoral Thesis - Department für Nanobiotechnologie (DNBT), BOKU-Universität für Bodenkultur, pp 182. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Abstract:
There is an increasing need in society for rapid, cost effective, and reliable detection of biomolecules for the purpose of diagnosing diseases, including early diagnosis of non-communicable and infectious diseases. Over the last years, these needs have been addressed by research in numerous biosensor technologies, where several key challenges must be addressed in order to enable accurate analysis of the low abundant species present in complex biological fluids. These challenges concern sensitivity and specificity in modalities that allow direct detection of the species without the need for extensive sample preparation steps and time-consuming (mostly enzymatic) amplification strategies. The thesis combines optical biosensing methods that take advantage of metallic structures with tailored plasmonic characteristics. The work is particularly focused on their utilization for signal amplification, multiplexing with the ability to achieve femtomolar detection limits for proteins and implementation of the biosensor into compact readout devices. First, plasmonic nanostructured surfaces were specially designed to be manufactured by scaled-up lithography methods and to be employed for weak optical signal amplification in surface plasmon resonance and surface plasmon-enhanced fluorescence spectroscopy readout of affinity binding events. Second, advanced biointerface architectures were developed in order to serve as an affinity binding matrix at the sensor surface. Several interdependent aspects of the sensor surface post-modification, of minimizing non-specific interactions and of additional (non-optical) signal enhancement were investigated. Finally, responsive polyelectrolyte brushes and poly[N-isopropylacrylamide (pNIPAAm)]-based hydrogels were used for sensitive detection of biomarkers in clinical saliva and plasma samples.

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