University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Logo BOKU Resarch Portal

Gewählte Master / Diploma Thesis:

Özcan Hir (2021): Verbesserter Biomasseaufschluss für die Energieträger der Zukunft – Biotreibstoffe und Biogas.
Master / Diploma Thesis - Institut für Landtechnik, BOKU-Universität für Bodenkultur, pp 69. UB BOKU obvsg FullText

Data Source: ZID Abstracts
One of the methods that increasingly applied is utilizing agricultural raw materials for sustainable and decentralized energy. Therefore, making use of straw as a raw material plays a pivotal role. Nevertheless, the structure of the lignocellulose biomass is resistant to enzymatic hydrolysis, which requires pre-treatment before the enzymatic hydrolysis. Taking energy efficiency and environmental factors into consideration, biological pre-treatment offers advantages over other methods. Although improvements are necessary, biological pre-treatments can be used more economically. This master thesis intends to examine an alternative method for the pre-treatment, and conversion of the plant biomass into a biologically usable raw material. For the pre-treatment of maize straw optimized Trichoderma reesei strains and white-rot fungi were inoculated together. To provide improvement and to achieve an optimized enzyme effect, the Trichoderma reesei strain was sexually crossed in succession. After that, four white-rote fungi were selected. Finally, T. reesei strains and white-rote fungi were inoculated for pre-treatment with maize straw in different periods from a few weeks to three months. The white-rote fungi are responsible for degrading of lignin, and Trichoderma reesei strains for that of cellulose. Although the maize straw has shown improvement in pre-treatment, the desired synergy between Trichoderma and white-rote fungi was not achieved. However, cocultivation for eight weeks of white-rote BH31 and Trichoderma reesei strain 1A2D15 showed a synergistic effect on the yield of glucose. This cocultivation reached the result of 187 mg per 1 g of maize straw after 6 hours of enzymatic hydrolysis. Similarly, with the same conditions, the achievement of glucose of unpretreated sample is 68 mg per 1 g of maize straw.

Beurteilende*r: Gronauer Andreas
1.Mitwirkender: Bauer Alexander
2.Mitwirkender: Basyouni-Khamis Samira

© BOKU Wien Imprint