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Gewählte Master / Diploma Thesis:

Tim Haban (2020): Histochemical location of key enzyme activities involved in stigmatic receptivity of Matricaria chamomilla L..
Master / Diploma Thesis - Institut für Pflanzenbau, BOKU-Universität für Bodenkultur, pp 88. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Abstract:
It is assumed that German chamomile (Matricaria chamomilla L., Asteraceae) exhibits sporophytic self-incompatibility. However, it has not yet been completely clarified if the incompatibility mechanism is indeed located on the stigma surface. In some plants, the incompatibility reactions are associated with inadequate penetration of the pollen through the cuticle of the stigma. For this reason, the activity of certain enzymes, such as cutinases, is believed to play an important role in the incompatibility reactions. The present work has been taken up to understand the relationship between stigma maturity and pollen receptivity in German chamomile. The primary objective was to determine whether the compatibility mechanism has an effect on the activity of the enzymes involved in stigmatic receptivity. Therefore, after repeated manual self-pollination, the interactions between pollen and stigma were made visible by staining enzymatic activities and then recorded under the microscope. Accordingly, stigmatic activity of esterases and peroxidases were identified in different floral development stages and compared between self-compatible and self-incompatible genotypes. In the course of floral development, there was generally a constant increase in stigmatic peroxidase activity, whereas a rather irregular development was observed with regard to the stigmatic esterase activity. Overall, the results show that there were no significant differences between the self-incompatible and the self-compatible genotypes. However, significant differences were found between individual genotypes, but these were rather due to a high genotypic variability and therefore did not occur exclusively between self-compatible and self- incompatible genotypes. Further research is needed to reveal more details about the incompatibility mechanism in German chamomile.

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