University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Gewählte Master / Diploma Thesis:
Celia Fernández Balado
(2021):
Conducting microcosm experiments to select suitable pollutant-degrading microbial consortia for perchloroethene remediation.
Master / Diploma Thesis - Institut für Bodenforschung (IBF),
BOKU-Universität für Bodenkultur,
pp 47.
UB BOKU
obvsg
FullText
- Abstract:
- Perchloroethene (PCE) is one of the most frequent contaminants found in groundwater. It is widely used as a solvent in the industry and its chemical properties make it a persistent and difficult to degrade pollutant. Reductive dichlorination is a biological remediation process in which organohalide respiring bacteria (OHRB) transform PCE to other toxic metabolites until the harmless ethene. This study investigated the dichlorination performance of two commercially available mixed microbial communities (KB1 and Bioclear b.v.) for further use in physical-biological remediation filters. Microcosm (MC) experiments and compound-specific isotope analysis (CSIA) were used to monitor PCE degradation, and a phospholipid fatty acid (PLFA) extraction was carried out to investigate the microbial community’s structure. Results showed that the type of electron source for the bacteria and the presence of Dehalococcoides sp. are key factors for the complete degradation of PCE. In addition, no significant PCE isotope fractionation could be observed, meaning that applying CSIA might not always be possible to quantify degradation. The KB1 culture, when incubated with lactate, is suitable for complete PCE dichlorination and represents a potential option for its use in combination with biochar for physical-biological remediation.
- Beurteilende*r: Watzinger Andrea
- 1.Mitwirkender: Leitner Simon
- 2.Mitwirkender: Keiblinger Katharina