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Gewählte Master / Diploma Thesis:

Marlene Niedermayer (2022): Screening of Environmentally Diverse Soils to Discover New Beneficial Microbes for Use in Sustainable Agriculture.
Master / Diploma Thesis - Institut für Bodenforschung (IBF), BOKU-Universität für Bodenkultur, pp 176. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Abstract:
As the human population is growing the need for food is also increasing, but in the face of climate change this should be done in the most sustainable way. This puts pressure on agriculture to achieve high yield with at least impact on the climate as possible. In recent years the possibility to use microbes to reach this goal gained more attention. During this project twelve natural Danish soils have been tested as a source for new beneficial microbes. Four soils showed the possibility of containing beneficial microbes after a first phenotypic screening. A nutrient rescue experiment showed that plants are nutrient deprived when they were grown in those candidate soils, especially when the microbes, that are naturally occurring in those soils, are absent. With one soil a transplantation experiment was done, to locate the microbes (either in bulk soil, rhizosphere or endosphere) that are positively impacting Arabidopsis thaliana. A so-called soil dilution experiment was done to reduce the microbes in the soil. Therefore, sterile soil was inoculated with a soil extract of the same site and the plant growth and phenotype was monitored and compared. Previously one soil was found to contain beneficial, endophytic bacteria, which have already been isolated. These were used in a mono-association assay with Arabidopsis thaliana where the root growth was compared to a control. One bacterium was found which significantly enhanced the plant weight and root length of the plant. For future experiments also four Arabidopsis thaliana mutants have been genotyped during this project. Those can be used to study the molecular mechanism of the plant-microbe interaction.

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