University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Gewählte Master / Diploma Thesis:
Maren Mellendorf
(2008):
Characterisation of the microbial community structure and 13C PLFA incorporation after vegetable oil application.
Master / Diploma Thesis,
BOKU-Universität für Bodenkultur,
pp 103.
UB BOKU
obvsg
- Abstract:
- Vegetable oil is used in soil remediation processes as a polycyclic aromatic hydrocarbon extraction (PAH) solvent and as carbon addition to stimulate microbiological PAH degradation. The influence of remaining vegetable oil on soil microorganisms was studied in three 13C PLFA experiments. The phospholipid fatty acid (PLFA) technique was used for the identification of soil microorganisms and community composition, whereas delta 13C isotopic measurements were carried out to determine the rate and temporal dynamics of C incorporation into microbial biomass. The application of two times vegetable oil increased microbial biomass and changed the community composition from a more complex (bacterial, fungi and actinomyceten) to a fungal dominant structure. 13C-labelling with maize oil indicated carbon incorporation rates up to 59 % after one maize oil application and up to 39% after two maize oil applications. A lower carbon substrate incorporation capacity was observed in the contaminated compared to the uncontaminated soil. 13C-Phenanthrene incorporation was observed for five PLFAs in the vegetable oil treated and for two PLFAs in the control oil soil. Rape oil application increased the total PLFA biomass similar to the maize oil applications in the first study. In conclusion, the biodegradation of remaining vegetable oil through soil microorganisms might be possible due to the increase in biomass, spezialisation of the soil microbial community structure and incorporation of carbon. The short experimental duration, however, did not indicate mineralization of the vegetable oil carbon.
- Beurteilende(r): Gerzabek Martin