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Gewählte Master / Diploma Thesis:

Thomas Kampitsch (2009): Molekularbiologische Identifizierung der Gattung Salix mittels HRM.
Master / Diploma Thesis, BOKU-Universität für Bodenkultur, pp 137. UB BOKU obvsg

Data Source: ZID Abstracts
Besides many other uses of willow (Salix spp.) by man, it is also an interesting plant for phytomedical use. But only a limited number of Salix species are allowed to be used in this field. In the genus Salix morphological and phytochemical identification is sometimes difficult, especially in processed raw materials. Therefore an identification based on DNA may improve the insufficient situation in identifying Salix species. High Resolution Melting Analysis (HRM) is an elegant technique in molecular biology that can be applied to differentiate Salix species. To develop an identification system, three chloroplast-primerpairs, corresponding to sequences in the tRNA-Ser-gene, the trnS-psb intergenic spacer tRNA-lys-gene for maturase K clon A, the psbZ-gene for photosystem II subunit Z and one primerpair from the Salix floricaula – gene, were used to perform the HRM analysis with the Corbett Rotorgene 6000 – device. A separation between pharmaceutically allowed and pharmaceutically not allowed species was possible, whereas clarification rate lies at approximately 50%. In either case the rate of potential mistakes drops considerably. By using additional primer pairs, this rate can be significantly improved.


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