University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Gewählte Doctoral Thesis:
Sohail Yousaf
(2011):
The ecology of alkane-degrading bacteria in phytoremediation of diesel fuel.
Doctoral Thesis - Institut für Bodenforschung (IBF),
BOKU-Universität für Bodenkultur,
pp 123.
UB BOKU
obvsg
FullText
- Abstract:
- We analyzed the hydrocarbon degradation potential and colonization of four alkane degrading strains (ITSI10, ITRI15, ITRH76, and BTRH79) in the rhizosphere and endosphere of Italian ryegrass and birdsfoot trefoil. Hydrocarbon degradation (up to 57 %) was observed in all inoculated treatments of vegetated and unvegetated samples. Italian ryegrass in combination with compost and BTRH79 showed highest degradation, while birdsfoot trefoil performed best with compost and strain ITSI10. The combination between vegetation, inoculation with well performing degrading bacteria and compost amendment was an efficient approach to reduce hydrocarbon contamination. The second part of the study aimed to assess the role of Enterobacterendophytes in hydrocarbon degradation,plant colonization, abundance and expression of CYP153 genes in different plant compartments, three plant species (Italian ryegrass, birdsfoot trefoil and alfalfa) were grown in sterilesoil spiked with 1% diesel and inoculated with three endophyticEnterobacterludwigii strains. We studies plant colonization of these strains as well as the abundance and expression of CYP153 genes in the rhizosphere, root and shoot interior. Results showed that all strains were capable of hydrocarbon degradation and efficiently colonizedthe rhizosphere and plant interior. Two strains, ISI10-3 and BRI10-9, showed highest degradation up to 68% and performed best in combination with Italian ryegrass and alfalfa. All strains principally expressed the CYP153 gene in all plant compartments, indicating an active role in degradation of diesel in various plant compartments.
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