University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

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Ernst Wolfgang

Scientific publications from BOKU scientists, especially those which are published in SCI / SSCI journals, or in other refereed journals, proceedings, monographs as well as articles in collected volumes are listed under PUBLICATIONS.

Publications with special focus on transfer of knowledge ("social impact") to practitioners as well as the interested public can be found under the menu item:

>> Transfer of Knowledge


** = Publications listed in SCI/SSCI - Journals (published in Web of Science)
* = peer-reviewed publication (not listed in SCI/SSCI)
Only: Full paper/article, Review, Proceedings Paper

= Open Access - Publications

 

2020

Journal Articles

** Ruzsics, Z; Hoffmann, K; Riedl, A; Krawczyk, A; Widera, M; Sertznig, H; Schipper, L; Kapper-Falcone, V; Debreczeny, M; Ernst, W; Grabherr, R; Hengel, H; Harant, H A Novel, Broad-Acting Peptide Inhibitor of Double-Stranded DNA Virus Gene Expression and Replication.

FRONT MICROBIOL. 2020; 11, 601555 WoS PubMed FullText FullText_BOKU

2019

** Koczka, K; Ernst, W; Palmberger, D; Klausberger, M; Nika, L; Grabherr, R Development of a Dual-Vector System Utilizing MicroRNA Mimics of the Autographa californica miR-1 for an Inducible Knockdown in Insect Cells.

INT J MOL SCI. 2019; 20(3), 533 WoS PubMed FullText FullText_BOKU

2018

** Koczka, K; Peters, P; Ernst, W; Himmelbauer, H; Nika, L; Grabherr, R Comparative transcriptome analysis of a Trichoplusia ni cell line reveals distinct host responses to intracellular and secreted protein products expressed by recombinant baculoviruses.

J BIOTECHNOL. 2018; 270: 61-69. WoS PubMed FullText FullText_BOKU

** Reinhart, D; Damjanovic, L; Castan, A; Ernst, W; Kunert, R Differential gene expression of a feed-spiked super-producing CHO cell line.

J BIOTECHNOL. 2018; 285: 23-37. WoS PubMed FullText FullText_BOKU

2014

** Maccani, A; Hackl, M; Leitner, C; Steinfellner, W; Graf, AB; Tatto, NE; Karbiener, M; Scheideler, M; Grillari, J; Mattanovich, D; Kunert, R; Borth, N; Grabherr, R; Ernst, W Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation.

APPL MICROBIOL BIOT. 2014; 98(17): 7535-7548. WoS PubMed PUBMED Central FullText FullText_BOKU

** Maccani, A; Landes, N; Stadlmayr, G; Maresch, D; Leitner, C; Maurer, M; Gasser, B; Ernst, W; Kunert, R; Mattanovich, D Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins.

BIOTECHNOL J. 2014; 9(4): 526-537. WoS PubMed PUBMED Central FullText FullText_BOKU

** Wilde, M; Klausberger, M; Palmberger, D; Ernst, W; Grabherr, R Tnao38, high five and Sf9-evaluation of host-virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression.

BIOTECHNOL LETT. 2014; 36(4): 743-749. WoS PubMed PUBMED Central FullText FullText_BOKU

2013

** Brinkrolf, K; Rupp, O; Laux, H; Kollin, F; Ernst, W; Linke, B; Kofler, R; Romand, S; Hesse, F; Budach, WE; Galosy, S; Muller, D; Noll, T; Wienberg, J; Jostock, T; Leonard, M; Grillari, J; Tauch, A; Goesmann, A; Helk, B; Mott, JE; Puhler, A; Borth, N Chinese hamster genome sequenced from sorted chromosomes.

NAT BIOTECHNOL. 2013; 31(8): 694-695. WoS PubMed FullText FullText_BOKU

** Maccani, A; Ernst, W; Grabherr, R Whole Genome Sequencing Improves Estimation of Nuclear DNA Content of Chinese Hamster Ovary Cells.

CYTOM PART A. 2013; 83(10): 893-895. WoS FullText FullText_BOKU

Conference & Workshop Proceedings, Paper, Abstract

Landes, N; Maccani, A; Leitner, C; Maurer, M; Gasser, B; Ernst, W; Kunert, R; Mattanovich, D. (2013): Cross-species comparison of recombinant protein secretion in CHO cells and Pichia pastoris. [Poster]
[7th Conference on Recombinant Protein Production RPP7 2013, Laupheim, Germany, 06.03.2013 - 08.03.2013]

In: European Federation of Biotechnology, RPP7-Abstract Book

Maccani, A., Tatto, N., Graf, A., Leitner, C., Mattanovich, D., Kunert, R., Ernst, W. (2013): Transcriptome study of high and low producing recombinant Chinese hamster ovary cell lines. [Poster]
[7th Conference on Recombinant Protein Production RPP7 2013, Laupheim, Germany, 06.03.2013 - 08.03.2013]

In: European Federation of Biotechnology, RPP7-Abstract Book

2012

Journal Articles

** Hernández Bort, JA; Hackl, M; Höflmayer, H; Jadhav, V; Harreither, E; Kumar, N; Ernst, W; Grillari, J; Borth, N; Dynamic mRNA and miRNA profiling of CHO-K1 suspension cell cultures..

Biotechnol J. 2012; 7(4):500-515 WoS PubMed FullText FullText_BOKU

Conference & Workshop Proceedings, Paper, Abstract

** Klausberger, M; Wilde, M; Palmberger, D; Ernst, W; Grabherr, R A novel virus-like particle-based anticancer vaccine using the mimotope strategy.
[European Congress of Immunology 2012, Glasgow, Scotland, UK, 05.09.2012 - 08.09.2012]

IMMUNOLOGY. 2012; 137: 754-754. WoS

2011

Journal Articles

** Grabherr, MG; Pontiller, J; Mauceli, E; Ernst, W; Baumann, M; Biagi, T; Swofford, R; Russell, P; Zody, MC; Di Palma, F; Lindblad-Toh, K; Grabherr, RM Exploiting Nucleotide Composition to Engineer Promoters.

PLOS ONE. 2011; 6(5): WoS FullText FullText_BOKU

** Thaisuchat, H; Baumann, M; Pontiller, J; Hesse, F; Ernst, W Identification of a novel temperature sensitive promoter in cho cells.

BMC BIOTECHNOL. 2011; 11: WoS FullText FullText_BOKU

* Baumann, M; Höppner, MP; Meier, M; Pontiller, J; Ernst, W; Grabherr, R; Mauceli, E; Grabherr, MG; (2011): Artificially designed promoters: understanding the role of spatial features and canonical binding sites in transcription..

Bioeng Bugs. 2011; 3(2):120-123 PubMed PUBMED Central FullText FullText_BOKU

2010

Monographs

Mäkelä, A. R., Ernst, W., Grabherr, R., Oker-Blom, C. (2010): Determination of recombinant baculoviral display viral titer.

pdb.top72.PMID: 20194463, Cold Spring Harb. Protoc.

Mäkelä, A. R., Ernst, W., Grabherr, R., Oker-Blom, C. (2010): Baculovirus-based display and gene delivery systems.

pdb.top72.PMID: 20194467, Cold Spring Harb. Protoc.

Mäkelä, A. R., Ernst, W., Grabherr, R., Oker-Blom, C. (2010): Monitoring baculovirus-mediated efficiency of gene delivery.

pdb.top72.PMID: 20194466, Cold Spring Harb. Protocol

Mäkelä, A. R., Ernst, W., Grabherr, R., Oker-Blom, C. (2010): Immunoelectron microscopy analysis of recombinant baculovirus display viruses.

pdb.top72.PMID: 20194465, Cold Sring harb. Protoc.

Mäkelä, A. R., Ernst, W., Grabherr, R., Oker-Blom, C. (2010): Creation of baculovirus display libraries.

, Cold Spring Harb. Protoc.

Mäkelä, A. R., Ernst, W., Grabherr, R., Oker-Blom, C. (2010): Immunofluorescence analysis of baculovirus displayed viral proteins in insect cells.

pdb.top72.PMID: 20194464, Cold Spring Harb. Protoc.

Journal Articles

** Baumann, M; Pontiller, J; Ernst, W; Structure and basal transcription complex of RNA polymerase II core promoters in the mammalian genome: an overview..

Mol Biotechnol. 2010; 45(3):241-247 WoS PubMed FullText FullText_BOKU

** Grabherr, R; Ernst, W; Baculovirus for eukaryotic protein display..

Curr Gene Ther. 2010; 10(3):195-200 WoS PubMed

** Pontiller, J; Maccani, A; Baumann, M; Klancnik, I; Ernst, W; Identification of CHO endogenous gene regulatory elements..

Mol Biotechnol. 2010; 45(3):235-240 WoS PubMed FullText FullText_BOKU

2009

** Schröfelbauer, B; Raffetseder, J; Hauner, M; Wolkerstorfer, A; Ernst, W; Szolar, OH Glycyrrhizin, the main active compound in liquorice, attenuates pro-inflammatory responses by interfering with membrane-dependent receptor signalling..

Biochem J. 2009; 421(3):473-482 WoS PubMed FullText FullText_BOKU

2008

* Trummer, E; Ernst, W; Hesse, F; Schriebl, K; Lattenmayer, C; Kunert, R; Vorauer-Uhl, K; Katinger, H; Müller, D (2008): Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysis..

Biotechnol J. 2008; 3(7):924-937 PubMed FullText FullText_BOKU

Conference & Workshop Proceedings, Paper, Abstract

** Pontiller, J; Gross, S; Thaisuchat, H; Hesse, F; Ernst, W Identification of CHO endogenous promoter elements based on a genomic library approach.

MOL BIOTECHNOL. MOLECULAR BIOTECHNOLOGY; 39: 135-139. (ISBN: Sao Paulo, Brazil) WoS PubMed FullText FullText_BOKU

2006

Journal Articles

** Ernst, W; Schinko, T; Spenger, A; Oker-Blom, C; Grabherr, R Improving baculovirus transduction of mammalian cells by surface display of a RGD-motif..

J Biotechnol. 2006; 126(2):237-240 WoS PubMed FullText FullText_BOKU

** Nakowitsch, S; Kittel, C; Ernst, W; Egorov, A; Grabherr, R; Optimization of baculovirus transduction on FreeStyle293 cells for the generation of influenza B/Lee/40..

Mol Biotechnol. 2006; 34(2):157-164 WoS PubMed FullText FullText_BOKU

* Ernst, W; Trummer, E; Mead, J; Bessant, C; Strelec, H; Katinger, H; Hesse, F; (2006): Evaluation of a genomics platform for cross-species transcriptome analysis of recombinant CHO cells..

Biotechnol J. 2006; 1(6):639-650 PubMed FullText FullText_BOKU

Conference & Workshop Proceedings, Paper, Abstract

Borth N., Brugger G., Hinterkörner G., Ernst W., Müller D., Hesse F., Kunert R., Katinger H. (2006): Cell Line Development by Fluorescence Activated Cell Sorting.
[Engineering Foundation Cell Culture Engineering X, Whistler, BC, Canada, 01.04.2006]

In: Engineering Foundation: Engineering Foundation Cell Culture Engineering X, April 2006, Whistler, Canada

Chapter in Collected Volumes

Mäkela, A., Ernst, W., Grabherr, R., Oker-Blom, C. (2006): Baculovirus-based display and gene delivery systems.

In: Rena Steuer, Gene TRansfer: Delivery and Expression of DNA and RNA, 1-10; Cold Spring Harbor Laboratory Press, New York, USA

Textbooks

Mäkelä, A., Ernst, W., Grabherr, R., Oker-Blom, C. (2006): Baculovirus-based display and gene delivery systems..

; Cold Spring Harbor Laboratory Press

2005

Journal Articles

** Böhm, E; Grillari, J; Voglauer, R; Gross, S; Ernst, W; Ferko, B; Kunert, R; Katinger, H; Borth, N Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting..

J Immunol Methods. 2005; 307(1-2):13-23 WoS PubMed FullText FullText_BOKU

** Grillari, J; Ajuh, P; Stadler, G; Löscher, M; Voglauer, R; Ernst, W; Chusainow, J; Eisenhaber, F; Pokar, M; Fortschegger, K; Grey, M; Lamond, AI; Katinger, H; SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly..

Nucleic Acids Res. 2005; 33(21):6868-6883 WoS PubMed PUBMED Central FullText FullText_BOKU

2004

** Ojala, K; Koski, J; Ernst, W; Grabherr, R; Jones, I; Oker-Blom, C Improved display of synthetic IgG-binding domains on the baculovirus surface..

Technol Cancer Res Treat. 2004; 3(1):77-84 WoS PubMed FullText FullText_BOKU

** Spenger, A; Ernst, W; Condreay, JP; Kost, TA; Grabherr, R Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells..

Protein Expr Purif. 2004; 38(1):17-23 WoS PubMed FullText FullText_BOKU

Conference & Workshop Proceedings, Paper, Abstract

Heinl, S., Gross, S., Ernst, W., grabherr, R. (2004): Evaluation and optimisation of different facilities of recombinant protein production in the baculovirus expression system.
[Joint Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT and ANGT 2004, Innsbruck, Austria, 20.09.2004 - 22.09.2004]

In: ÖGBT/ÖGGGT: Joint Meeting of ÖGBM; ÖGGGT; ÖGBT; ANGT, 20.-22. Sept. 2004, Innsbruck

Schinko, T., Toellner, L., Ernst, W., Grabherr, R. (2004): Surface modification of baculovirus for improved transduction efficiency of mammalian cells..
[Joint Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT and ANGT 2004, Innsbruck, Austria, 20.09.2004 - 22.09.2004]

In: ÖGBT/ÖGGGT: Joint Meeting ÖGBM/ÖGGGT/ÖGBT/ANGT, 20.-22. Sept. 2004, Innsbruck

2003

Journal Articles

** Poomputsa, K; Kittel, C; Egorov, A; Ernst, W; Grabherr, R Generation of recombinant influenza virus using baculovirus delivery vector..

J Virol Methods. 2003; 110(1):111-114 WoS PubMed

Conference & Workshop Proceedings, Paper, Abstract

** Grabherr, R; Poomputsa, K; Kittel, C; Toellner, L; Ernst, W; Egorov, A Generation of recombinant influenza virus using baculovirus as a delivery vehicle..

ABSTR PAP AMER CHEM SOC. 2003; 225: 227-BIOT WoS

Spenger, A., Ernst, W., Kost, T., Grabherr, R. (2003): Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells..
[Protein expression in animal cells, Mont-Tremblant, QC, Canada, 09.09.2003 - 11.09.2003]

In: PEACe: Protein expression in animal cells, 9.-11. Sept. 2003, Mont Tremblant, Canada

2002

Journal Articles

** Ecker, GF; Csaszar, E; Kopp, S; Plagens, B; Holzer, W; Ernst, W; Chiba, P Identification of ligand-binding regions of P-glycoprotein by activated-pharmacophore photoaffinity labeling and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry..

Mol Pharmacol. 2002; 61(3):637-648 WoS PubMed

** Spenger, A; Grabherr, R; Töllner, L; Katinger, H; Ernst, W Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64..

Eur J Biochem. 2002; 269(18):4458-4467 WoS PubMed

2001

** Grabherr, R; Ernst, W; The baculovirus expression system as a tool for generating diversity by viral surface display..

Comb Chem High Throughput Screen. 2001; 4(2):185-192 WoS PubMed

** Grabherr, R; Ernst, W; Oker-Blom, C; Jones, I; Developments in the use of baculoviruses for the surface display of complex eukaryotic proteins..

Trends Biotechnol. 2001; 19(6):231-236 WoS PubMed

Conference & Workshop Proceedings, Paper, Abstract

Spenger, A., Töllner, L., Ernst, W., Grabherr, R., (2001): Altering the surface properties of baculovirus AcNPV by insertional mutagenesis of the envelope protein gp64.
[Gene delivery and cellular protein expression conference, Semmering, Austria, 09.09.2001 - 13.09.2001]

In: PEACe: Gene delivery and cellular protein expression conference, 9.-13. Sept. 2001, Semmering, Austria

2000

Journal Articles

** Ernst, WJ; Spenger, A; Toellner, L; Katinger, H; Grabherr, RM Expanding baculovirus surface display. Modification of the native coat protein gp64 of Autographa californica NPV..

Eur J Biochem. 2000; 267(13):4033-4039 WoS PubMed FullText FullText_BOKU

Conference & Workshop Proceedings, Paper, Abstract

Grabherr, R., Ernst, W. (2000): Baculovirus Surface Display“: Establishment and Applications.
[Dechema Biotechnologie 2000, Berlin, Germany, 01.09.2000]

In: Dechema Biotechnologie 2000: Dechema Biotechnologie 2000, Sept. 2000, Berlin, Germany

1999

Grabherr, R., Ernst, W. (1999): Baculovirus Surface Display: Development, Optimization and Future Aspects“.
[Phage Club Meeting, La Grande-Motte, France, 01.05.1999]

In: Phage Club Meeting: Phage Club Meeting, Mai 1999, La Grande-Motte, France

1998

Journal Articles

** Ernst, W; Grabherr, R; Wegner, D; Borth, N; Grassauer, A; Katinger, H Baculovirus surface display: construction and screening of a eukaryotic epitope library..

Nucleic Acids Res. 1998; 26(7):1718-1723 WoS PubMed PUBMED Central FullText FullText_BOKU

1997

** Grabherr, R; Ernst, W; Doblhoff-Dier, O; Sara, M; Katinger, H Expression of foreign proteins on the surface of Autographa californica nuclear polyhedrosis virus..

Biotechniques. 1997; 22(4):730-735 WoS PubMed

1994

** Ernst, WJ; Grabherr, RM; Katinger, HW Direct cloning into the Autographa californica nuclear polyhedrosis virus for generation of recombinant baculoviruses..

Nucleic Acids Res. 1994; 22(14):2855-2856 WoS PubMed PUBMED Central

Doctoral Thesis

Ernst, W. (1994): Baculoviren als Expressionsvektoren für heterologe Proteine..

Dissertation, Universität für Bodenkultur Wien

1993

Conference & Workshop Proceedings, Paper, Abstract

Ernst, W., Grabherr, R., Katinger, H. (1993): Direct cloning into the Autographa californica nuclear polyhedrosis virus (AcMNPV) genome for generation of recombinant baculoviruses..

Common Annual meeting of ÖGGGT and ÖBG, Vienna

1992

Chapter in Collected Volumes

Ernst, W., Kohl, J., Rüker, F., Katinger, H. (1992): Expression of a single-chain antibody (anti HIV-1 gp 41) in insect cells..

In: Vlak, J., Schlaeger, E., Bernard, A. (eds.): Baculovirus and recombinant protein production processes. Basel: Editiones Roche

1990

Journal Articles

* Reiter, M; Weigang, F; Ernst, W; Katinger, HW (1990): High density microcarrier culture with a new device which allows oxygenation and perfusion of microcarrier cultures..

Cytotechnology. 1990; 3(1):39-42 PubMed

1989

Master / Diploma Thesis

Ernst, W. (1989): Spezifische Umsatzraten von Aminosäuren und Glucose in kultivierten Hybridomas..

Diplomarbeit, Universität für Bodenkultur Wien

1988

Conference & Workshop Proceedings, Paper, Abstract

Katinger, H. W. D., Reiter, M., Weigang, F., Ernst, W., Doblhoff-Dier, O., Borth, N., Steindl, F. (1988): A scalable modular vibrating cage system for enhanced gas/liquid diffusion and cell immobilisation in continous perfused culture of mammalian cells..

Abstracts Engineering Foundation Conference on Cell Culture Engineering, Palm Coast, Florida, USA

Reiter, M., Schwengler, P., Ernst, W., Weigang, F., Steindl, F., Doblhoff-Dier, O., Borth, N., Katinger, H. W. D. (1988): Physiological patterns of cultured hybridomas: Metabolic flux rates of amino acids and substrates of the glycolytic chain..

Abstracts Engineering Foundation Conference on Cell Culture Engineering, Palm Coast, Florida, USA

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