Florian Rüker
Ao.Univ.Prof.i.R. Dr. Florian Rüker
Institut Institute of Molecular Biotechnology
Location Muthgasse 11, 1190 Wien
Email florian.rueker@boku.ac.at
Tel: +43 1 47654-79854
ORCID: 0000-0001-7639-2686
7004170860: AuthorId
Career
- 2020 - 2022 Head of Institute of Molecular Biotechnology
- 2016 - 2019 stv. Vorsitzender der Fachstudienkommission Lebensmittel- und Biotechnologie, BOKU
- 2016 - 2020 Deputy Head of the Department of Biotechnology
- 2014 - 2016 Head of the Department of Biotechnology
- 2009 - 2013 Deputy Head of the Department of Biotechnology
- 2009 - 2015 Head, curriculum committee Food- and Biotechnology, BOKU
- 2006 Incorporation of f-star Forschungs- und Entwicklungs GmbH together with colleagues
- 1994 Habilitation, University of Agricultural Sciences, Vienna
- 1986 Promotion, University of Agricultural Sciences, Vienna
- 1982 Sponsion, University of Agricultural Sciences, Vienna
Awards
- 2011 science2business award - 1. Preis
- 2010 Houska Preis - 2. Platz
- 2008 science2business award - 2. Preis
Projects
0 Projects found.
Publications
Knowledge Transfer to Society
Media
Lectures
(2023) Combining Controlled Fab-arm exchange and mAb² for the production of asymmetric trispecific antibodies
Autoren: Natale, V; Stadlmayr, G; Benedetti, F; Stadlbauer, K; Rüker, F; Wozniak-Knopp, G
PEGS Europe
(2023) Controlled Fab-arm exchange on mAb² pairs for the production of multispecific antibodies
Autoren: Natale, V; Stadlmayr, G; Benedetti, F; Stadlbauer, K; Rüker, F; Wozniak-Knopp, G
PEGS Boston
(2022) Trispecific Antibodies produced from mAb² pairs by controlled Fab Arm exchange
Autoren: Natale, V; Stadlmayr, G; Benedetti, F; Stadlbauer, K; Rüker, F; Wozniak-Knopp, G
Antibody Engineering & Therapeutics Europe
(2022) Circumventing immunization - The use of in vitro evolution and selection to make antibodies
Autoren: Rüker, F;
ÖGMBT-Young Life Scientists Austria/FEBS Junior Sections - Talk Series 2022
(2022) CD81 large extracellular loop as a novel alternative scaffold: laminin binding CD81 enhances specific uptake of extracellular vesicles
Autoren: Wozniak-Knopp, G; Vogt, S; Stadlmayr, G; Stadlbauer, K; Bobbili, MR; Kjems, J; Rüker, F; Grillari, J
9th BBBB International Conference on Pharmaceutical Sciences 2022
(2022) Circumventing immunization - The use of in vitro evolution and selection to make monoclonal antibodies
Autoren: Rüker, F;
Behördenseminar Bundesministerium für Bildung, Wissenschaft und Forschung Sektion V - Wissenschaftliche Forschung; Internationale Angelegenheiten Referat 3b - Gentechnik und Tierversuchswesen
(2021) Circumventing immunization by in vitro directed evolution and selection of human antibodies from large surface display libraries
Autoren: Rüker, F;
Gesellschaft zur Förderung von alternativen Biomodellen (The RepRefRed Society)
(2021) Bispecific antibodies – new formats for cancer immunotherapy
Autoren: Rüker, F;
BioSeparation Forum
(2021) An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: laminin binding CD81 enhances cellular uptake of extracellular vesicles
Autoren: Vogt, S; Bobilli, MR, Stadlmayr, G; Stadlbauer, K; Rüker, F; Wozniak-Knopp G
Austrian Society for Extracellular Vesicles
(2019) Improving the stability of the large extracellular loop of human tetraspanin CD81
Autoren: Stefan Vogt, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Rüker, Johannes Grillari, Gordana Wozniak-Knopp
International Society of Extracellular Vesicles - Annual Meeting 2019
(2019) Laminin-targeted extracellular vesicles reveal enhanced specific uptake in vitro
Autoren: Stefan Vogt, Madhusudhan Reddy Bobbili, Florian Rüker, Johannes Grillari, Gordana Wozniak-Knopp
1st Annual Symposium of the Danish Society for Extracellular Vesicles (DSEV)
(2017) Construction of a naive yeast-display Fab antibody library
Autoren: Stadlmayr, G; Eibensteiner, K; Rüker, F; Wozniak-Knopp, G
Merck Biopharma Antibody Engineering ?Mini-Symposium
(2016) Antibody microarray for fast detection of methicillin-resistant Staphylococcus aureus
Autoren: Krobath, E; Nöhammer, .; Rüker, F; Wozniak-Knopp, G; Hust, M; Wiesinger-Mayr, H
Austrian Biomarker Symposium 2016
(2016) Domain-Exchanged Antibody with potential for improved effector functions
Autoren: Wozniak-Knopp, G; Perthold, JW; Stadlmayr, G; Stadlbauer, K; Rüker, F.
8th ÖGMBT Annual Meeting 2016
(2016) Domain-Exchanged Antibody with potential for improved effector functions
Autoren: Wozniak-Knopp, G; Perthold, JW; Stadlmayr, G; Stadlbauer, K; Rüker, F.
The 5th Thailand International Nanotechnology Conference
(2016) Domain-exchanged antibody with potentiated effector functions
Autoren: Knopp, GW; Stadlmayr, G; Perthold, JW; Stadlbauer, K; Rueker, F
8th ÖGMBT Annual Meeting 2016
(2016) Domain-Exchanged Antibody with Potentiated Effector Functions
Autoren: Wozniak-Knopp, G; Perthold, JW; Stadlmayr, G; Stadlbauer, K; Rüker, F
17th European Biotechnology Congress
(2015) Engineering bispecific antibodies using the SEED platform: Solving the light chain pairing problem
Autoren: Dietrich, S; Alec W. Gross, AW; Wozniak-Knopp, G; Becker, S; Hock, B; Rüker, F;
7th ÖGMBT Annual Meeting 2015
(2015) Novel bispecific SEED antibodies with specific light chain pairing
Autoren: Dietrich, S; Gross, AW; Wozniak-Knopp, G; Becker, S; Hock B; Rüker, F;
The 1st Biologics & Biosimilars Congress
(2015) Engineering bispecific antibodies using the SEED platform: Solving the light chain pairing problem
Autoren: Dietrich, S; Gross, AW; Wozniak-Knopp, G; Becker, S; Hock, B; Rüker, F;
7th ÖGMBT Annual Meeting 2015
(2015) Creating a new Fab interface in bispecific antibodies using rational design and small mammalian cell-based libraries
Autoren: Bönisch, M; Sevo, M; Becker, S; Hock, B; Rüker, F;
8th Conference on Recombinant Protein Production RPP8 2015
(2015) Construction of a very large semi-synthetic human antibody library
Autoren: Gerhard Stadlmayr, Gordana Wozniak-Knopp, Milica Ševo, Florian Rüker
Merck Serono Antibody Engineering ?Mini-Symposium
(2015) Engineering novel antibody biologics
Autoren: Rüker, F;
Woche der Chemie 2015
(2015) Engineering Novel Antibody Biologics by Modular Antibody Technology
Autoren: Rüker, F; Wozniak-Knopp, W;
Wilhelm Exner Lectures
(2015) Rational design of a new Fab interface for the generation of heterodimeric IgGs
Autoren: Bönisch, M; Sevo, M; Becker, S; Hock, B; Rüker, F;
The Biologics Congress
(2015) scFv-Fc fusion-protein Microarrays for fast detection of methicillin-resistant Staphylococcus aureus
Autoren: Krobath, E; Wozniak-Knopp, G; Hust, M; Rüker, F; Nöhammer, C; Wiesinger-Mayr, H
7th Annual PEGS Europe Protein and Antibody engineering summit 2015
(2015) Modulares Antikörper Engineering
Autoren: Rüker, F; Wozniak-Knopp, G;
Young Science-Initiative
(2015) Bispecific antibodies targeting VEGF and ErbB receptors
Autoren: Wozniak-Knopp, G; Stadlmayr, G; Stadlbauer, K; Steger, F; Rüker, F
Affinity Proteomics Workshop
(2014) Molecular antibody technology: novel formats and applications of antibodies with engineered antigen-binding constant domains
Autoren: Rüker, F; Wozniak-Knopp, G;
Human Antibodies and Hybridomas Conference 2014
(2014) Modular Antibody Technology
Autoren: Wozniak-Knopp, G; Rüker F
Bioprocess International 2014
(2014) Advancing novel antibody biologics
Autoren: Rüker, F;
11th International Life Science Meeting 2014
(2014) Stability Assessment of Antigen binding Fc fragments (Fcabs) of Different Library Designs
Autoren: Ravnikar, M; Wozniak-Knopp, G; Rüker, F
6th ÖGMBT Annual Meeting 2014 - Life Sciences meet Entrepreneurship
(2014) Construction of pH-sensitive Her2 binding antibody fragment by directed evolution using yeast display
Autoren: Lobner, E., Traxlmayr, M. W., Rueker, F., Obinger, C.
The 16th European Congress on Biotechnology
(2013) Strategies for the stabilization of Fc fragments
Autoren: Ruker, F; Knopp, GW
European Biotechnology Congress 2013
(2013) Elemental labeled antibody fragments for LC-ICP-MS based immunoassays
Autoren: Mairinger, T., Kretschy, D., Wozniak-Knopp, G. Rueker, F., Koellensberger, G. and Hann, S.
Metallomics 2013, 4th International symposium on metallomics
(2013) Engineering the Stability of Antibody Constant Domains
Autoren: Wozniak-Knopp, G; Rüker, F;
Affinity 2013
(2013) Fcabs – antigen binding Fc regions
Autoren: Rüker, F; Wozniak-Knopp, G;
6th Alpbach Workshop on Affinity Proteomics
(2013) Engineering and Ensuring Stability of Antibodies and Antibody Fragments
Autoren: Rüker, F; Wozniak-Knopp, G;
Optimize 2013 - Antibody Engineering & Discovery
(2013) Engineering and Ensuring Stability of Antibodies and Antibody Fragments
Autoren: Rüker, F;
Optimize 2013 - Antibody Engineering & Discovery
(2013) Fcabs - Antigen Binding Fc Regions
Autoren: Rüker, F;
6th Alpbach Workshop on Affinity Proteomics
(2013) World-class biotech research base in Austria
Autoren: Rüker, F;
BIO Europe 2013 - High-Level Programme for International Delegations
(2013) Strategies for the Stabilisation of Fc fragments
Autoren: Rüker, F; Wozniak-Knopp, G;
European Biotechnology Congress 2013
(2012) Modular antibodies: Introducing antigen-binding sites in the Fc region of IgG
Autoren: Ruker, F
Eurobiotech Agriculture Symposium 2012
(2012) Strategies for the stabilization of Fc fragments
Autoren: Gordana Wozniak-Knopp, Johannes Stadlmann, Florian Rüker
FA0804 COST Action Molecular farming 2012 -Plants as a production platform for high value proteins
(2012) Stabilization of the Fc fragment of human IgG1 by engineered interdomain disulfide bonds
Autoren: Wozniak-Knopp, G; Stadlmann, J; Rüker, F;
9th International Conference on Protein Stability 2012 - From molecular level to market applications
(2012) Stability engineering of IgG1-Fc and construction of a “protein stability landscape” by high throughput sequencing
Autoren: Traxlmayr, M.W.Faissner, M., Hasenhindl, C., Hackl, M., Stadlmayr, G., Rüker, F. and Obinger, C.
9th International Conference on Protein Stability 2012 - From molecular level to market applications
(2012) Systematic analysis of the C-terminal loops of IgG1-Fc for their usability in Modular Antibody Engineering
Autoren: Hasenhindl, C., Wozniak-Knopp1, G., Traxlmayr, M.W., Hackl, M., Stadlmayr, G., Rüker F. and Obinger C.
9th International Conference on Protein Stability 2012 - From molecular level to market applications
(2012) Pre-clinical developments for bispecific mAb2s with optimal target selection
Autoren: Rüker, F;
Protein and Antibody Engineering Summit PEGS Europe 2012
(2012) Measures to Enhance Half-Life and Stability (Preconference Short Course)
Autoren: Rüker, F; Traxlmayr, M;
Protein and Antibody Engineering Summit PEGS Europe 2012
(2012) Stability engineering of human IgG1-Fc to resist boiling
Autoren: Stadlmayr, G., Schaffner, I., Traxlmayr, M., Wozniak-Knopp, G., Hasenhindl, C., Rüker, F. and Obinger, C.
9th International Conference on Protein Stability 2012 - From molecular level to market applications
(2012) F-Star Cambridge - Spinoff company from BOKU
Autoren: Rüker, F;
ICA RECTORS & DEANS FORUM 2012 - Engagement of life science universities in the knowledge society and innovation networks
(2011) Stability engineering of the Fc fragment of human IgG1 by targeted mutagenesis
Autoren: Stadlmayr, G., Wozniak-Knopp, G., Hasenhindl, C., Rüker, F., Obinger, C.
6th Conference on Recombinant Protein Production RPP6 2011
(2011) Stability-engineering of human IgG1-Fc by using yeast display
Autoren: Traxlmayr, M. W., Faissner, M., Stadlmayr, G., Rüker, F., Obinger, C.
PEGS - Protein and Antibody Engineering Summit 2011
(2011) Stability engineering of human IgG1-Fc and application in modular antibody engineering
Autoren: Hasenhindl,C., Traxlmayr, M. W., Faissner, M., Stadlmayr, G., Rüker, F., Obinger, C.
3rd PEGS Europe Protein & Antibody Engineering Summit 2011
(2010) Stabilization of the Fc fragment of human IgG1 by engineered intra-domain disulfide bonds
Autoren: Gordana Wozniak-Knopp, Gerhard Stadlmayr, Florian Rüker
24th Anual Symposium of The Protein Society 2010
(2010) Engineering antigen binding sites into the Fc region of IgG: biochemical, biophysical and immunological characterization
Autoren: Florian Rüker, Gordana Wozniak-Knopp
24th Anual Symposium of The Protein Society 2010
(2010) Stability engineering of the Fc fragment of human IgG1 by targeted mutagenesis
Autoren: Stadlmayr, G., Wozniak-Knopp, G., Hasenhindl, C., Rüker, F., Obinger, C.
5th Central European Conference on Chemistry meets biology
(2010) Engineering antigen binding sites into the Fc region of IgG
Autoren: Florian Rüker, Gordana Wozniak-Knopp
Advancing Protein Therapeutics
(2010) Stabilization of the Fc fragment of human IgG1 by engineered intra-domain disulfide bonds
Autoren: Gordana Wozniak-Knopp, Gerhard Stadlmayr, and Florian Rüker
2nd ÖGMBT Annual Meeting 2010
(2010) Introducing antigen-binding sites into structural loops of immunoglobulin constant domains
Autoren: Gordana Wozniak-Knopp, Stefan Bartl, Anton Bauer, Marwa Mostageer, Max Woisetschläger, Bernhard Antes, Karl Ettl, Manuela Kainer, Gertrude Weberhofer, Susanne Wiederkum, Gottfried Himmler, Geert Mudde, and Florian Rüker
2nd ÖGMBT Annual Meeting 2010
(2009) Fcab and mAb2: biologically active and antigen-specific immunoglobulin Fc molecules derived from yeast display libraries with antigen binding sites in the IgG1-CH3 domain
Autoren: Florian Rüker
Protein Engineerung Summit 2009
(2009) Immunoglobulin Fc as a Scaffold for Antigen Binding (Fcabs):Long Half Life and Effector Functions
Autoren: Gordana Wozniak-Knopp
Protein Engineering Summit Europe 2009
(2009) Fcab and mAb2 Biologically active and antigen-specific immunoglobulin Fc molecules derived from yeast display libraries with antigen binding sites in the IgG1-CH3 domain
Autoren: Florian Rüker
3rd International Symposium on Antibody Engineering and Antibody-based Therapeutics (AEAT 2009)
(2009) Engineered human IgG1-Fc binding to integrin
Autoren: Traxlmayr, M., Wozniak-Knopp, G., Rüker, F., Obinger, C.
20th IBC's Annual International Conference on Antibody Engineering 2009
(2008) Introducing Modular Antibody Technology
Autoren: Florian Rüker
Antibody Engineering Asia 2008
(2007) Introducing Modular Antibody Technology
Autoren: Florian Rüker
IBC’s 18th Annual International Antibody Engineering Conference 2007
(2005) Protein Engineering am IAM
Autoren: Florian Rüker
60 Year Anniversary, Institute of Applied Microbiology IAM 2005
(2005) Cloning, Expression, Purification and Characterization of human Alpha-Fetoprotein
Autoren: Michael Dekner, Gordana Wozniak-Knopp and Florian Rüker
60 Year Anniversary, Institute of Applied Microbiology IAM 2005
(2005) Selection and characterization of phage-display scFvs directed against Ep-CAM
Autoren: Gordana Wozniak-Knopp and Florian Rüker
60 Year Anniversary, Institute of Applied Microbiology IAM 2005
(2003) Engineering catalase-peroxidases: The structural basis of the activity of catalase-peroxidases.
Autoren: Petutschnig, G., Jakopitsch, C., Auer, M., Rüker, F., Furtmüller, P. G., and Obinger, C.
Eigth International Summer School on Biophysics
(2003) Do we really understand the catalatic reactivity?
Autoren: Jakopitsch, C; Petutschnig, G; Zehner, F; Ruker, F; Furtmuller, PG; Obinger, C
Plant Stress, Reactive Oxygen and Antioxidants
(2003) Converting Myeloperoxidase to Lactoperoxidase
Autoren: Zederbauer, M., Ciaccio, C., Moguilevsky, N., Jantschko, W., Furtmüller, P. G., and Obinger, C.
Eigth International Summer School on Biophysics
(2002) Cloning and hererologous expression of Sclerotium rolfsii CDH gene in Pichia pastoris with its own signal peptide.
Autoren: Zamocky, R. Ludwig, F. Rüker, and D. Haltrich
6th European Conference on Fungal Genetics 2002
Probing active site residues of catalase-peroxidases.
Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek , G.A., Obinger, C.
Tackling the light chain association problem in heterodimeric IgG
Autoren: Bönisch, M; Dietrich, S; Sevo, M; Quendler, H; Becker, S; Günther, R; Hock, B; Rüker, F
Catalase versus peroxidase activity. Towards understanding the bifunctional activity of catalase-peroxidases.
Autoren: Regelsberger, G., Jakopitsch, C., Rüker, F., Loewen, P., Peschek, G. A., Obinger, C.
Characterization of the superoxide dismutases in the filamentous cyanobacterium Anabaena variabilis.
Autoren: Regelsberger, G., Laaha, U., Dagmar, D., Rüker, F., Peschek, G.A., Obinger, C.
Cloning, overexpression and characterization of a cyanobacterial monofunctional catalase.
Autoren: Schwaiger, H., Regelsberger, G., Rueker, F., Peschek, G.A., Zámocky, M., Obinger, C.
Manipulating the distal asparagine-histidine couple in catalase-peroxidase.
Autoren: Jakopitsch, C., Regelsberger, G., Rücker, F., Peschek, G.A., Obinger, C.
Iron and manganese containing cyanobacterial superoxide dismutases.
Autoren: Regelsberger, G., Laaha, U., Dietmann, D., Rüker, F., Peschek, G.A., Obinger, C.
Hydrogen peroxide removal in Anabaena variabilis: Cloning, overexpression and characterization of a thioredoxin peroxidase.
Autoren: Plasser, L., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.
Catalase versus peroxidase activity. Towards understanding the bifunctional activity of catalase-peroxidases.
Autoren: Regelsberger, G., Jakopitsch, C., Rüker, F., Peschek, G.A., Switala, J., Loewen, P., Obinger, C.
The cyanobacterial aa3-type cytochrome c oxidase: Biochemical characterization and expression of the Synechocystis sp. PCC6803 cta genes in Rhodobacter sphaeroides.
Autoren: Paumann, M., Hiser, C., Rüker, F., Obinger, C., Ferguson-Miller, S., Peschek, G.A.
Binding of peroxides and inhibitors to the active site of catalase-peroxidases.
Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.
The catalatic acitivity of catalase-peroxidases.
Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.
Stability assessment on a library scale – A rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.
Autoren: Hasenhindl, C., Traxlmayr, M.W., Wozniak-Knopp, G ., Jones, P.C., Stadlmayr, G., Rüker, F., Obinger, C.
Molecular engineering of catalase-peroxidases.
Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.
Binding of peroxides and inhibitors to the active site of catalase-peroxidases.
Autoren: Jakopitsch, C., Regelsberger, G., Rüker, F., Peschek, G.A., Obinger, C.
The molecular and functional peculiarities of class I peroxidases.
Autoren: Obinger, C., Regelsberger, G., Jakopitsch, C., Peschek, G.A., Rüker, F.
Directed evolution of antibody fragments for improved stability and resistance to aggregation by using yeast surface display
Autoren: Lobner, E., Traxlmayr, M. W., Rüker, F., Obinger, C.
Molecular and kinetic characterization of cyanobacterial iron- and manganese-containing superoxide dismutases.
Autoren: Regelsberger, G., Laaha, U., Dietmann, D., Rüker, F., Peschek, G.A., Obinger, C.
Hydroperoxidases in oxygenic phototrophic prokaryotes- comparison to plant chloroplasts
Autoren: Regelsberger, G., Jakopitsch, C., Rücker, F., Peschek, G.A., Obinger, C.
Cloning, overexpression and characterization of a cyanobacterial thioredoxin peroxidase.
Autoren: Plasser, L., Regelsberger, G., Rueker, F., Peschek, G. A., Obinger, C.