Electroporation als Methode, um funktionelle Membranproteine in Säugerzellen zu transferieren - ohne den Weg einer genetischen Manipulation zu beschreiten.

We propose developing a method of delivering a membrane protein to the cell membrane using electroporation instead of genetic manipulation. This method will combine and exploit three powerful technologies currently used in the field of synthetic biology: (i) in vitro membrane-assisted protein synthesis; (ii) the use of polymers in creating artificial membranes as scaffolds for protein folding and integration; (iii) the use of electroporation to fuse artificial membranes with cell membranes. Development of this technique will involve establishing protocols for electroporation, then tracing and understanding the fate of membrane proteins delivered to cell membranes using this method.