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Analytical µ-Platform for Automated Cell Factory Screening

Causon Tim, Projektleiter/in
University Relations Grant
Art der Forschung
Mattanovich Diethard, Sub-Projektleiter/in
Hann Stephan, Sub-Projektleiter/in
Beteiligte BOKU-Organisationseinheiten
Institut für Analytische Chemie (DCH/AC)
Institut für Mikrobiologie und Mikrobielle Biotechnologie
Gefördert durch
Agilent Technologies, Inc., 5301 Stevens Creek Blvd, 95051 Santa Clara, Vereinigte Staaten (USA)
Strain and process improvement is one of the most labor-intensive and time-consuming phases of biotechnology process development. High-throughput screening is usually in miniaturized static cultures which compromises scalability, so that further intermediate screening steps are needed. Maturing of existing technology to deal with additional cell culture types (i.e. mammalian cells) and incorporate further analytical developments to support metabolite and product screening requirements are identified as the key steps to creating a technology of commercial value. In this project, we propose to mature an existing µ-screening platform with two major pillars addressing these steps. Firstly, extending the applicability of the module to fermentation technology based on Chinese Hamster Ovary (CHO) cultures. Secondly, to expand the analytical value of the platform using a combination of valve technology and mass spectrometry to develop a fully integrated platform suitable for application in biotechnology facilities.
Analytische Chemie; Fermentation (404004); Industrielle Biotechnologie;
Biotechnology; µFluidics; Massenspektrometrie; Metabolomics; Zellfabriken;
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