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S-layer templated nanoparticles and carbon nanotubes arrays (AFOSR)

Projektleitung
Sleytr Uwe B., Projektleiter/in
Laufzeit:
15.04.2003-30.11.2006
Art der Forschung
Angewandte Forschung

Weitere Informationen: http://www.boku.ac.at/zuf

Mitarbeiter*innen
Beteiligte BOKU-Organisationseinheiten
Institut für Biophysik
Gefördert durch
Air Force Office of Scientific Research, Wilson Blvd. 4015, VA 22203-1954 Arlington, Vereinigte Staaten (USA)
Abstract
Das Ziel dieses Forschungsprojektes liegt in der Entwicklung einer Technologiebasis beruhend auf kristallinen bakteriellen S-Schichtenproteinen (S-Schichten) zur Herstellung geordneter Strukturen von metallischen Nanopartikeln und Bürsten von Kohlenstoff-Nanoröhren. Wir wollen hier gezielt die Fähigkeit von S-Schichtproteinen zur Ausbildung geordneter Monoschichten auf festen Trägern mit den neuartigen elektronischen und optischen Eigenschaften dieser funktionellen Nanomaterialien kombinieren. Nachdem die Nutzung der extremen Eigenschaften von Nanoröhren bislang an der Herstellung geordneter Strukturen gescheitert ist, wollen wir das Wachstum von Kohlenstoff-Bürsten durch regelmäßig gebundene metallische nanometergroße Katalysatorteilchen induzieren. Die Verwendung einer regelmäßigen Proteinschicht zur Herstellung geordneter Strukturen aus Nanopartikeln oder Kohlenstoff-Nanoröhren ist wissenschaftlich völlig neu. Nach letztem Stand der Technik haben konventionelle Methoden im Vergleich zur S-Schichttechnologie nicht die Vielfalt an Möglichkeiten in der Herstellung dieser Strukturen.
Schlagworte
Molekulare Nanotechnologie (Nanoengineering);
Nanopartikel; S-Schichten;
Publikationen

Egelseer, E.M., Ilk, N., Pum, D., Messner, P., Schäffer, C., Schuster, B., Sleytr, U.B. (2010): S-Layers, Microbial, Biotechnological Applications.

In: Flickinger, M.C. (Ed.), Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, pp. 4424-4448; Wiley-VCH Verlag, Weinheim, Germany

Messner, P., Schäffer, C., Egelseer, E.M., Sleytr, U.B. (2010): Occurrence, structure, chemistry, genetics, morphogenesis, and functions of S-layers.

In: H. König, H. Claus, and A. Varma (Eds.), Prokaryotic cell wall compounds - structure and biochemistry, pp. 53-109; Springer-Verlag, Berlin, Germany

Sleytr, U.B., Egelseer, E.M., Ilk, N., Messner, P., Schäffer, C., Pum, D., Schuster, B. (2010): Nanobiotechnological Applications of S-Layers.

In: H. König, H. Claus, A. Varma (Eds.) , Prokaryotic Cell Wall Components – Structure and Biochemistry, pp. 459-481; Springer Verlag, Heidelberg, Germany

** Badelt-Lichtblau, H., Kainz, B., Völlenkle, C., Egelseer, E.M., Sleytr, U.B., Pum, D., Ilk, N. Genetic engineering of thr S-layer protein SbpA of Lysinibacillus sphaericus CCM 2177 for the generation of functionalized nanoarrays.

BIOCONJUGATE CHEM, 20, 895-903; ISSN 1043-1802 WoS PubMed FullText FullText_BOKU

** Ilk, N; Egelseer, EM; Ferner-Ortner, J; Kupcu, S; Pum, D; Schuster, B; Sleytr, UB Surfaces functionalized with self-assembling S-layer fusion proteins for nanobiotechnological applications.

COLLOID SURFACE A. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; 321: 163-167. WoS FullText FullText_BOKU

Egelseer, E.M., Sára, M., Pum, D., Schuster, B., Sleytr, U.B. (2008): Genetically engineered S-layer proteins and S-layer-specific heteropolysaccharides as components of a versatile molecular construction kit for applications in nanobiotechnology.

In: Shoseyov, O., Levy, I. (Eds.) , Nanobiotechnology, pp. 55-86; Humana Press , Totowa, NJ

** Ferner-Ortner, J; Mader, C; Ilk, N; Sleytr, UB; Egelseer, EM High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology..

J Bacteriol. 2007; 189(19):7154-7158 WoS PubMed PUBMED Central FullText FullText_BOKU

** Sleytr, UB; Egelseer, EM; Ilk, N; Pum, D; Schuster, B; S-Layers as a basic building block in a molecular construction kit..

FEBS J. 2007; 274(2):323-334 WoS PubMed FullText FullText_BOKU

** Huber, C; Liu, J; Egelseer, EM; Moll, D; Knoll, W; Sleytr, UB; Sára, M; Heterotetramers formed by an S-layer-streptavidin fusion protein and core-streptavidin as a nanoarrayed template for biochip development..

Small. 2006; 2(1):142-150 WoS PubMed FullText FullText_BOKU

** Martín-Molina, A; Moreno-Flores, S; Perez, E; Pum, D; Sleytr, UB; Toca-Herrera, JL Structure, surface interactions, and compressibility of bacterial S-layers through scanning force microscopy and the surface force apparatus..

Biophys J. 2006; 90(5):1821-1829 WoS PubMed PUBMED Central FullText FullText_BOKU

** Schuster, B; Pum, D; Sára, M; Sleytr, UB; S-layer proteins as key components of a versatile molecular construction kit for biomedical nanotechnology..

Mini Rev Med Chem. 2006; 6(8):909-920 WoS PubMed FullText FullText_BOKU

** Toca-Herrera, JL; Küpcü, S; Diederichs, V; Moncayo, G; Pum, D; Sleytr, UB Fluorescence emission properties of S-Layer enhanced green fluorescent fusion protein as a function of temperature, pH conditions, and guanidine hydrochloride concentration..

Biomacromolecules. 2006; 7(12):3298-3301 WoS PubMed FullText FullText_BOKU

Sára, M., Pum, D., Huber, C., Ilk, N., Pleschberger, M., Sleytr, U.B. (2006): Nanoscale patterning of S-layer proteins as a natural self-assembly system.

In: Kumar, C., Biological and Pharmaceutical Nanomaterials, 219-252; Wiley-VCH, Weinheim, Germany

** Dieluweit S., Pum, D., Sleytr, U.B., Kautek, W. Monodisperse gold nanoparticles formed on bacterial crystalline surface layers (S-layers) by electroless deposition.

Mat Sci Eng C-Bio S, 25, 727-732 WoS FullText FullText_BOKU

Altmutter, C., Pum, D., Naik, R.R., Sleytr, U.B., Sara, M. (2005): Bottom-up approach based on S-layers as templates in the formation of ordered arrays of metallic nanoparticles.
[TNT2005 – Trends in Nanotechnology, Oviedo, Spain, August]

In: Phantoms Foundation: TNT2005 – Trends in Nanotechnology, August, Oviedo, Spain FullText

** Sleytr, UB; Pum, D; Sara, M; Schuster, B S-layer based nanostructures..
[229th National Meeting of the American Chemical Society ACS 2005, San Diego, CA, USA, 13.03.2005 - 17.03.2005]

ABSTR PAP AMER CHEM SOC. 2005; 229: 057-COLL WoS

Sleytr, U.B., Pum, D., Schuster, B., Sára, M. (2005): Nanobiotechnological Applications of S-Layers.
[Bionanotechnology 3, Keynote Lecture, Falmer, UK, September]

In: Royal Society of Chemistry: Bionanotechnology 3, Keynote Lecture, September, University of Sussex, Sussex, England

Sleytr, U.B., Pum, D., Schuster, B., Sára, M. (2005): Nanobiotechnologial applications of S-layers.
[NanoBionics III – from Molecules to Applications, Marburg, Germany, April]

In: Philipps-Universität Marburg: NanoBionics III – from Molecules to Applications, April, Schloss Rauischholzhausen, Marburg, Germany

Sleytr, U.B., Pum, D., Schuster, B., Sára, M. (2005): Nanobiotechnology with S-layers as basic building blocks.
[229th National Meeting of the American Chemical Society ACS 2005, San Diego, CA, USA, 13.03.2005 - 17.03.2005]

In: American Chemical Society: 229th American Chemical Society National Meeting,Polymeric Materials: Science and Engineering, Keynote Lecture, March, San Diego, CA

Sleytr, U.B., Sára, M., Pum, D., Schuster, B. (2005): Crystalline bacterial cell surface layers (S layers): A versatile self-assembly system.

In: Ciferri, A., Supramolecular Polymers, Second Edition, 583-616; CRC Press, Taylor & Francis Group, Boca Raton, FL; ISBN 0-8247-2331-7

Sleytr, U.B., Pum, D., Sára, M., Schuster, B. (2004): S-layer based nanostructures.
[Material Research Society MRS Fall Meeting 2004, Symposium Z, Boston, MA, USA, 01.11.2004]

In: Material Research Society: MRS Fall Meeting 2004, Symposium Z, November, Boston, MA; Proc. Material Research Society , p. 592

Sleytr, U.B., Schuster, B., Sára, M., Pum, D. (2004): S-layers as basic building block for a molecular construction kit.
[AH1 Biological, Nanostructures, Materials, and Applications Symposium, 206th Meeting of the Electrochemical Society, Honolulu, HI, USA, October]

In: Electrochemical Society: AH1 Biological, Nanostructures, Materials, and Applications Symposium, 206th Meeting of the Electrochemical Society, October, Honolulu, Hawaii

Sleytr, U.B., Egelseer, E.-M., Pum, D., Schuster, B. (2004): S-Layers.

In: Niemeyer, C.M., Mirkin, C.A. (Eds.), NanoBiotechnologie: Concepts, Methods and Perspectives, 77-92; Wiley-VCH Verlag, Weinheim, Germany; ISBN 3-527-30658-7

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