Functional analysis of the Arabidopsis At1g64110 AAA+ ATPase1
Abstract
Transcriptome analysis of syncytia induced by the cyst nematode Heterodera schachtii in Arabidopsis roots found that an AAA+ ATPase gene (At1g64110) was strongly expressed in syncytia. This gene is a member of a small gene family of three genes in Arabidopsis. Previous results have shown that this gene is important for syncytium development and abiotic stress responses. In this project we will further study the expression of this gene with antibodies. The subcellular location of the ATPases will be analysed using tags and antibodies. Since nothing is known today about the function of this ATPase, we will use T-DNA mutants and overexpression lines to address it´s possible function. AAA+ ATPases are widespread in plants and proteins with sequence similarity are also found in yeast. We will therefore use a complementation analysis of yeast mutants to find the function for this AAA+ ATPase. This should also shed light on the role of this protein in syncytia.
syncytium Heterodera schachtii Arabidopsis ATPase
Publikationen
Project staff
Holger Bohlmann
Assoc. Prof. Dr. Holger Bohlmann
holger.bohlmann@boku.ac.at
Project Leader
01.09.2014 - 30.06.2019