Re-building the Crabtree phenotype in yeast
Abstract
Being a fundamental feature of yeast metabolism, the Crabtree phenotype describes whether a yeast can perform aerobic fermentation at a higher glycolytic flux. It provides Crabtree positive yeasts evolutionary benefits to outcompete other microorganism as these yeasts are able to consume glucose faster and produce ethanol in sugar rich environments. It requires a complex deregulation of glycolysis and fermentation pathways as well as TCA cycle. A number of events were found to be contributed to the emergence of the Crabtree phenotype: whole genome duplication (WGD), rewiring of the transcriptional network, horizontal transfer of the URA1 gene, loss of respiratory complex I (CI), and duplication of hexose transporter genes; but the initial molecular mechanisms that promoted its evolution remains unresolved. Hypothesis: A single mutation of CRA1 of K. phaffii was the first step towards evolving a strong Crabtree positive phenotype by providing evolutionary benefits in the microbial populations without requiring more complex rewiring of the carbon metabolism.
yeast Komagataella phaffii aerobic fermentation evolution
Publikationen
What makes Komagataella phaffii non-conventional?
Autoren: Ata, O; Ergun, BG; Fickers, P; Heistinger, L; Mattanovich, D; Rebnegger, C; Gasser, B Jahr: 2021
Journal articles
Project staff
Diethard Mattanovich
Univ.Prof. Dipl.-Ing. Dr.nat.techn. Diethard Mattanovich
diethard.mattanovich@boku.ac.at
Tel: +43 1 47654-79006
Project Leader
01.10.2020 - 31.10.2023