Molecular biological identity control of micro-organisms used in silage
Abstract
Recently, lactic acid bacteria are widly used as starter cultures in the production of silage to ensure a high and constant quality of the final product. Micro-organisms of different origin are to be characterized by phenotypic methods as well as using modern genotypic procedures to prove the identity of the strains for further application. The comparison of whole-cell protein patterns obtained by highly standardised sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) should allow fast screening of large numbers of strains at the species level. To control the identity of strains, two DNA-based genotypic methods should be used. The rapid, accurate and budget-priced RAPD-PCR, which combines the advantages of DNA-fingerprinting and PCR, and pulsed-field gel electrophoresis. PFGE combined with controlled restriction by rare-cutting endonucleases has been used for strain differentiation and chromosome size estimation in lactic acid bacteria. As this technique showed a very high discriminatory power, PFGE restriction patterns are a powerful tool for classification and identification of strains.
silage micro-organisms protein fingerprinting DNA-fingerprinting RAPD-PCR PFGE
Publikationen
Project staff
Helmut Mayer
Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Helmut Mayer
helmut.mayer@boku.ac.at
Tel: +43 1 47654-75412
Project Leader
01.11.1999 - 31.10.2000
Rudolf Braun
Ao.Univ.Prof.i.R. Dipl.-Ing. Dr.rer.nat. Rudolf Braun
rudolf.braun@boku.ac.at
Project Staff
01.11.1999 - 31.10.2000