Organization of glycosylation enzymes in the Golgi apparatus
Abstract
The endomembrane system is an internal membrane system that controls secretion and uptake of biomolecules in all eukaryotic cells. At the heart of the endomembrane system is the Golgi apparatus, which receives cargo from the endoplasmic reticulum and sorts and delivers the cargo to various organelles within a cell. Within the secretory pathway the plant Golgi apparatus is the major biosynthetic organelle harbouring a large number of enzymatic activities for modification of proteins, lipids and for cell wall polysaccharide biosynthesis. Morphologically, the plant Golgi apparatus is a polarized structure consisting of flattened cisternae organized into stacks, which compartmentalize the components for the biosynthesis and trafficking/sorting of proteins, carbohydrates and lipids. Correct function of the Golgi apparatus is therefore essential for plant viability and consequently for the whole ecosystem on earth. Despite this importance little is known about cellular mechanisms that control the organization of the Golgi and its biosynthetic machinery. An unsolved fundamental question of cell biology is how the functional integrity of the Golgi is maintained while facing a constant flow of secretory products. Cargo is transported through the Golgi and modified within different cisternae, while Golgi-resident enzymes have to be retained or retrieved and organized along the different cisternae in order to maintain their biosynthetic function. In this project I will address this exciting question and try to unravel the molecular mechanisms involved in the sub-Golgi distribution of glycosylation enzymes in plants.
glycobiology plant cell biology plant physiology Golgi apparatus glycosyltransferases
Publikationen
Project staff
Richard Strasser
Assoc. Prof. Dr. Richard Strasser
richard.strasser@boku.ac.at
Tel: +43 1 47654-94345
BOKU Project Leader
01.01.2012 - 01.11.2016
BOKU partners
External partners
Oxford Brookes University
Prof. Chris Hawes
partner